Millennium Science Pty Ltd
ABN 83 006 389 466

Unit 2, 390 Canterbury Road
Surrey Hills VIC 3127
Australia

Telephone:  +61 3 9830 7922
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1800 678 242
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0800 507 896

Email: sales@mscience.com.au
Web:   www.mscience.com.au

Millennium Science is appointed Australian and New Zealand Distributors for Panomics by Affymetrix - click here to view the press release

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The Odyssey® Infrared Imaging System

Overview & Advantages

•  Wide Linear Range

Using fluorescently labeled antibodies rather than an enzymatic reaction, the Odyssey Imager offers a broad linear dynamic range to accurately detect strong and weak signals on the same Western blot.

• Two-Color Detection and Quantification

Simultaneous detection of two targets on the same membrane increases the accuracy of quantification and comparison.
Western blot analysis

        [ABOVE] ERK1/2 and phospho-ERK were detected
        simultaneously in lysates of unstimulated and
        EGF-stimulated A431 cells. Two fold serial dilutions
        of lysate were loaded. Psuedo-color images can be
        overlaid (yellow indicates overlap of red and green).
        The mobility shift caused by phosphorylation can
        be seen in the EGF-stimulated lysate.

        [ABOVE] Two-fold serial dilutions of Alexa® 680-labeled
        antibody (6 ng to 0.19pg) were spotted on nitrocellulose and
        imaged in the 700 nm channel.

• High Sensitivity

Near-infrared fluorescence provides equal or better sensitivity than chemiluminescence – giving you low background, high signal-to-noise, multiplexed detection, and the sensitivity of any fluorescent system.

      The Infrared Advantage

Most fluorescent imaging systems use visible fluorescence.

          o  Membranes and plastics can produce high background in the visible region, due to autofluorescence and
              light scatter.
          o This limits the sensitivity of visible fluorescence and makes it nearly impossible to detect low-abundance
              proteins.
          o The Odyssey uses near-infrared fluorescence, dramatically reducing autofluorescence and light scatter.

LI-COR Biosciences is the clear leader in fluorescent Western blot detection and near-infrared imaging, offering:

          o  Unique family of IRDye® Infrared Dyes with reactive functionalities for easy coupling to antibodies and
              other biomolecules.
          o  Wide selection of IRDye secondary antibodies, blocking buffers, and blotting membranes for fluorescent
              Western blot detection.
          o  Compatibility of the Odyssey Imager with many other fluorescent reagents.

• Direct Detection

With Odyssey system, there is no film, darkroom, plastic wrap, or substrates. IRDye signals on membranes are stable indefinitely if stored properly.

• Flexible System with Many Applications

The Odyssey system has been used for many applications, including Western blot analysis, EMSA, protein arrays, In-Cell Western™ Assays, On-Cell Westerns, in vivo imaging, Coomassie gel documentation, DNA gel documentation and tissue section analysis.

Odyssey data have been widely published in the literature, in journals such as Science, Nature, PNAS, Journal of Cell Biology, Journal of Biological Chemistry, Cancer Research, PLos ONE, and many others.

For more info visit http://www.licor.com/bio