Affymetrix Microarray Technology

With more than 1,860 installed systems worldwide and nearly 20,000 peer-reviewed publications, the GeneChip® System is the platform of choice for analyzing complex genetic information. Researchers use the data generated by our products to explore the relationship between genes and diseases, find and develop new drugs, and develop diagnostic tests.

Affymetrix microarray solutions include all necessary components for a microarray experiment, from arrays and reagents to instruments and software.

The new GeneTitan™ System, for example, is the world's first hands-free, automated microarray processing system that reduces labor costs and improves data consistency. Researchers can now take a whole-genome approach to expression profiling and genotyping while smoothly scaling up to process large numbers of samples.

Affymetrix microarray solutions include all necessary components for a successful microarray experiment, from arrays and reagents to instruments and software. Our complete range of solutions addresses diverse research needs, from RNA to DNA analysis, a few to thousands of samples, and targeted to genome-wide analysis.

DNA Analysis Solutions

COPY NUMBER ANALYSIS

Affymetrix provides a suite of products designed for researchers exploring the role of copy number in human disease.

The new Affymetrix Genome-Wide Human SNP Array 6.0 features 1.8 million genetic markers, including more than 906,600 single nucleotide polymorphisms (SNPs) and more than 946,000 probes for the detection of copy number variation. The SNP Array 6.0 is the only platform with analysis tools to truly bridge copy number and association, including a new, high-resolution reference map and a copy number polymorphism (CNP) calling algorithm developed by the Broad Institute. The SNP Array 6.0 demonstrates industry-leading performance and represents more genetic variation on a single array than any other product, providing maximum panel power and the highest physical coverage of the genome.

CYTOGENETICS

Traditional cytogenetics techniques such as karyotyping and fluorescent in situ hybridization (FISH) have been used to study chromosomal abnormalities for decades. These techniques are limited to only providing low-resolution copy number information based on qualitative visualization. Designed to empower next-generation cytogenetic studies, the CytoScan® HD Cytogenetics Solution provides a genome-wide approach that enables high-resolution DNA copy number analysis to detect gains, losses, loss of heterozygosity (LOH), regions identical-by-descent, and uniparental isodisomy (UPD) on a single array.

  • The most comprehensive and relevant coverage of constitutional and cancer genes on a single array
    coverage of all 36,000 RefSeq genes including 12,000 OMIM, all ISCA constitutional regions, and Sanger cancer genes
  • High-density genotype-able SNPs
    for low-level mosaicism visualization, loss of heterozygosity (LOH) detection, copy number change confirmation, and genotypes
  • Performance that exceeds community guidelines
    specificity, sensitivity, and resolution across the genome
  • Complete solution that saves time and money
    streamlined protocols, optimized assay performance, bundled reagents, easy-to-use software, DVD and laboratory-based training
  • Proven ChAS Software with genotypes
    intuitive, graphical, and streamline analysis software
  • Highest performance with the broadest range of sample types
    blood, bone marrow, buccal saliva, fresh and frozen tissue, fresh and cultured cells, and more

The complete cytogenetics research solution

Used in combination with the GeneChip® Instrument System, the next-generation Affymetrix CytoScan® HD Cytogenetics Solution provides everything you need to do cytogenetics research.

GENOME-WIDE GENOTYPING

Affymetrix is a leading provider of microarray solutions for targeted and genome-wide applications. Our powerful portfolio includes arrays, reagents, instruments, and informatics tools that enable you to detect common and rare single nucleotide polymorphisms (SNPs), copy number variants, and other genetic variations that can contribute to complex diseases.

AXIOM™ GENOTYPING SOLUTION

The Axiom Genotyping Solution is Affymetrix' newest product for genome-wide association studies (GWAS), replication studies, and candidate gene association studies. It includes predesigned and customized array plates with validated genomic content from the Axiom Genomic Database. This end-to-end solution also includes complete reagent kits, data analysis tools, and a fully automated workflow utilizing the GeneTitan® Multi-Channel (MC) Instrument.

Benefits of the Axiom™ Genotyping Solution

  • Flexible content - optimize your studies with a diverse set of relevant and novel variants
  • Fast results - process more than 760 samples per week with less than 18 hours of hands-on time
  • More power - run more samples within your budget
  • Greater confidence - generate reliable results with end-to-end automated sample processing and fully optimized reagent kits

>>Read More

Instruments and Software

GENE TITAN

The GeneTitan® family of instruments and proven GeneChip® Array Plates from Affymetrix are the first to provide a hands-free, automated solution for monitoring gene expression and genome-wide SNP genotyping.

The GeneTitan Instrument and the GeneTitan® Multi-Channel (MC) Instrument automate array processing from target hybridization to data generation by combining a hybridization oven, fluidics processing, and state-of-the art imaging device into a single bench-top instrument.

The imaging device in the GeneTitan MC Instrument uses an external, high-intensity xenon lamp and dual excitation and emission filters to capture images from array plates for use in genome-wide expression and genotyping studies. The imaging device in the single-channel GeneTitan Instrument uses an internal LED and a single emission filter to acquire data from the industry-leading expression array plates for genome-wide expression profiling.

The GeneTitan® family of instruments provides the highest throughput and laboratory productivity per technician by minimizing user intervention and allowing unattended, overnight processing of large numbers of samples in parallel. This unique degree of hands-free automation means your data will be consistently reproducible, your lab will be more productive, and you can spend less time acquiring and managing data and have more time for science.

Key benefits

  • Easy - Designed for operators with minimal microarray experience
  • Flexible - Supports gene expression and genotyping studies on 16-, 24- and 96-format array plates
  • Accurate - Delivers high-quality, consistent data every time
  • Efficient - Condenses hands-on processing time to as little as 30 minutes, images an array in less than five minutes, and operates unattended overnight
  • Robust - Has fewer moving parts so it's easily maintained and rarely requires IT support
  • Scalable - Meets both medium- and high-throughput needs
  • Adaptable - Creates flexible workflows and sample registration via Affymetrix® GeneChip® Command Console® (AGCC) Software

GENE ATLAS

Virtually every life scientist would like a whole-genome view of complex biological questions. Many, however, are inhibited by budget or the complexity of microarray systems, and are forced to use less powerful technologies with a limited view of the genome.

Now you can take control of your samples, complete your experiment more quickly than ever, and apply the power of Affymetrix microarrays to your research, in your lab, at a price you can afford.

The GeneAtlas System utilizes an entirely new array format-the array strip, with four arrays per strip. The array strip design and the GeneAtlas System combine to provide distinct advantages:

  • Simplified hybridization - no need to create a hybridization chamber assembly
  • Robustness that protects precious samples, unlike fragile slide-based designs
  • Limited hands-on time
  • Simple array processing with common microwell-based labware

With the easy-to-use GeneAtlas System, you can move seamlessly through array processing to your destination: understanding your data. Affymetrix has partnered with Partek and Ariadne to develop workflow-driven statistical and pathway analysis software packages with the biologist in mind, so you get biologically relevant results, not just data.

The GeneAtlas System includes:

  • Hybridization station
  • Fluidics station
  • Imaging station
  • Computer workstation with monitor
  • GeneAtlas instrument control software
  • External barcode reader
  • GeneAtlas installation kit

GENECHIP SCANNER

The GeneChip® Instrument System is a fully integrated platform for conducting your research using GeneChip® brand probe arrays. Affymetrix offers three versions of this system.

The GeneChip® Scanner 3000 7G System includes the GeneChip® Scanner 3000 7G, Fluidics Station 450, Hybridization Oven 645 and a powerful computer workstation with quad-core Xeon processors loaded with Affymetrix® GeneChip® Command Console® Software (AGCC). This system can be purchased with or without a GeneChip® AutoLoader.

The GeneChip® Scanner 3000 7G Whole-Genome Association System is designed for use with the Affymetrix SNP Assay 6.0. This system includes the GeneChip Scanner 3000 7G Plus with AutoLoader, two Fluidics Station 450s, Hybridization Oven 645 and a powerful computer workstation with quad-core Xeon processors loaded with Affymetrix GeneChip Command Console Software (AGCC). The system comes with a 2D handheld barcode reader for the instrument control workstation.

The GeneChip® Scanner 3000 Targeted Genotyping System is designed for use with Affymetrix GeneChip® Custom Genotyping assays and GeneChip® probe arrays. The system includes the four-color GeneChip Scanner 3000 7G Plus with AutoLoader, two Fluidics Station 450s, Hybridization Oven 645, a powerful computer workstation with quad-core Xeon processors loaded with Affymetrix GeneChip Command Console Software (AGCC), pre-amp and post-amp workstations with GeneChip® Targeted Genotyping Analysis Software and a 2D handheld barcode reader with each workstation.

Molecular Diagnostics

GENECHIP® SYSTEM3000Dx

The GeneChip® System 3000Dx (GCS 3000Dx) is the first microarray instrumentation system for molecular diagnostic laboratories. The system consists of the Fluidics Station 450Dx, which automates hybridization, washing and staining of the array; the GCS 3000Dx Scanner with AutoLoader Dx, which allows you to load 48 microarrays to be scanned without operator intervention; and a Workstation with GCOS Dx software.

The GCS 3000Dx is 510(k)-cleared and CE-marked for in vitro diagnostic use in conjunction with the Roche Diagnostics AmpliChip CYP450 Test. The AmpliChip CYP450 Test is the first microarray-based diagnostic test and the first product to be released through the Powered by Affymetrix™ partnership between Affymetrix and Roche Diagnostics.

GENECHIP® SYSTEM 3000Dx v.2

The Affymetrix GeneChip® System 3000Dx v.2 (GCS 3000Dx v.2) is Affymetrix' second-generation solution for seamless DNA and RNA analysis.

The GCS 3000Dx v.2- which is 510(k) cleared and CE marked for in vitro diagnostic use- consists of the GeneChip® Scanner 3000Dx v.2, the GeneChip® Fluidics Station 450Dx v.2 and Workstation with Affymetrix Molecular Diagnostics Software (AMDS).

Please contact us for more information about these Molecular Diagnostics systems.

RNA Analysis Solutions

3' IVT EXPRESSION ANALYSIS

Build on the knowledge of thousands of publications with 3' IVT solutions-the industry-standard tool for gene expression research.

PrimeViewTM (U219) Human Gene Expression Cartridge Arrays

Affymetrix now offers the U219 3' expression array, PrimeView Human Gene Expression Array, in cartridge format. PrimeView was created utilizing the most recent genome annotation build (UniGene database 219 and RefSeq version 36) and is focused on high confidence gene content. PrimeView includes >553,000 probes to detect in total > 43,000 well established coding transcripts*. PrimeView is not backwards compatible with HG-U133 arrays nor the whole transcript, Gene 1.0 ST array.

Key benefits of the PrimeViewTM Array:

  • Offers excellent gene expression specificity and reproducibility
  • Produces reliable results with multiple independent measurements per transcript-11 probes per set for wellannotated sequences, 9 probes per set for the remainder.
  • Provides complete coverage of the annotated genome
    *Highest transcript coverage in the industry with the most recent annotation-covers all well-annotated genes and transcripts from RefSeq
We are offering Introductory promotion from now until 30th December 2011 - Buy a min of 2 PrimeView products and get 25% off the total PrimeView order (PROMO CODE: AMQ311145)

PrimeView products include:

AFX-901837 GeneChip® PrimeView™ Human Gene Expression Array contains 10 arrays

AFX-901838 GeneChip® PrimeView™ Human Gene Expression Array contains 30 arrays

AFX-901839 GeneChip® PrimeView™ Human Gene Expression Array and 3' IVT Express Kit Bundle* sufficient for 10 reactions

AFX-901840 GeneChip® PrimeView™ Human Gene Expression Array and 3' IVT Express Kit Bundle* sufficient for 30 reactions

*Arrays can be purchased under this promotion without the 3'IVT Express kit.

GENE REGULATION ANALYSIS

GeneChip® Tiling Arrays are the most comprehensive array tools available for discovering novel RNA transcripts or mapping sites of protein/DNA interaction in chromatin immunoprecipitation experiments.

miRNA ANALYSIS

GENECHIP ® miRNA V2.0 ARRAY

The GeneChip® miRNA Array offers the most comprehensive coverage of miRNAs on a single array. Combined with a free software tool and labeling kits from Genisphere, the complete miRNA solution is a powerful tool for exploring the role of miRNAs.

Benefits of the GeneChip® miRNA Array

  • Comprehensive content - Provides complete coverage of miRBase V15, including snoRNA and scaRNA probes
  • Unique content - Designed to independently interrogate pre-miRNA and mature miRNA in the same experiment
  • High sensitivity - Detects 85 percent of miRNA transcripts at 1.0 amol
  • High reproducibility - 0.95 reproducibility (inter- and intra-lot)
  • Easiest workflow - 45-minute assay without pre-amplification or purification steps
  • Low sample input - Requires as little as 100 ng total RNA
  • Affordable - Meets the budget needs of every life science researcher

WHOLE-TRANSCRIPT EXPRESSION ANALYSIS

Get the most complete view of the genome with Affymetrix' line of whole-transcript expression products.