Biocare Medical

Immunohistochemistry • Antibodies • Automation

IntelliPATH FLX®

Using a suite of advanced technologies for increased productivity and turnaround time, the intelliPATH FLX is the intelligent choice for anatomic pathology and research laboratories.

The intelliPATH FLX is a fully open system with continuous random access and prioritized STAT capability, delivering maximum flexibility to meet the needs of your laboratory. A suite of high performance technologies includes simultaneous multiplex IHC capability, "no touch" liquid level sensing, and simultaneous X and Y movement to help provide the most rapid turnaround time and sheer volume of IHC results available.

Whether your workflow involves running large slide batches, discrete small batches, or a combination of batch and STAT slides, the intelliPATH FLX delivers the right solution with an innovative slide staining system, optimized reagents, and award-winning technical support for a variety of applications.

  • Flexible, fully open system
  • Convenience, productivity & ease of use
  • Simultaneous Multiplex IHC
  • Continuous random access
  • High speed & throughput
  • Connect & manage

Declocking Chamber

The Decloaking Chamber™ Plus offers a combination of complete walk-away and quality control capabilities, ensuring optimal antigen retrieval with superior and consistent results.

This system features a cooling fan for more rapid antigen retrieval, producing results in as little as 35 minutes. An optional stainless steel rack holder allows easy removal of plastic slide containers. Furthermore, a USB port enables the laboratory to utilize a flash drive to track and save run data for quality control purposes. The pressure gauge has a new feature in which the needle remains at the highest pressure reading during the run, validating that antigen retrieval has occurred properly. When the procedure is complete, an audible beep alerts the user.

The Decloaking Chamber™ Plus, with up to 96-slide capacity, is well suited for high-throughput antigen retrieval.

Standardized Results

  • Crisp and even staining
  • Very gentle on tissues
  • Reproducible results
  • Reduction of false negatives

A Complete Quality Control System

  • HIER buffer, pH indicators change color with temperature
  • Two-step deparaffinization and retrieval buffers
  • Steam monitor strips for temperature verification
  • Audible alert upon run completion

Detection

Biocare Medical Offer a large range of detection systems for the immunohistochemistry scientist. See which ones suit your needs:

4 plus

A sensitive two-step HRP and AP detection method

Biocare's 4plus detection systems are designed for reliable, cost-effective, two-step detection, providing a high-level of sensitivity. The vast majority of primary antibodies can be diluted two-fold compared to other commercially available avidin-biotin detection systems. Biocare's prediluted antibodies are all standardized to work with the 4plus detection system.

4plus detection system can be used with mouse and rabbit primary antibodies. After labeling the antigen with primary antibody, a species-specific or universal, affinity-purified, biotinylated secondary antibody is added to bind. Horseradish peroxidase (HRP) or alkaline phosphatase (AP) labeled-streptavidin is then added. A chromogen/substrate is applied that reacts with a HRP or AP to produce an intense color signal.

Chromogens

Biocare Medical offers the largest variety of IHC-specific permanent chromogens, for use with the two most commonly used enzyme labels: Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Chromogens are chemical compounds used within IHC staining procedures that produce a colored end-product that can be visualized under bright-field microscopy.

Fluorescence

Biocare's Signal Amplification Reagent increases fluorescent signals 3 to 5-fold when used in combination with DyLight immunofluorescent detection kits. This increased sensitivity is perfect for identification of low abundance proteins as well as routine work. Amplify your staining intensity for nuclear, cell membrane, and cytoplasmic antigens with Biocare's Fluorescence Enhancement Probe technology. For use on human tissue only.

  • 3 to 5-fold Signal amplification with Biocare DyLight Detection Kits
  • Superior to FITC and TRITC
  • Multiplex detection protocols

MACH 1™

The MACH 1 Universal Polymer Detection system offers a cost-effective alternative method of polymer detection for IHC laboratories (not available in the U.S.). Specifically titered for Biocare prediluted antibodies, MACH 1 provides ­superior staining and a reduced protocol time as compared to the conventional ABC ­system.

This new two-step, biotin-free technology uses a specific probe to detect mouse antibodies. The probe is followed by an HRP-polymer that binds to both the probe and to rabbit antibodies. Time consuming avidin-biotin and normal serum blocking steps are eliminated. The innovative HRP-polymer technology provides superior staining for mouse and rabbit antibodies when compared to ABC, biotin-streptavidin (BSA) and other polymer detection kits.

MACH 2™

MACH 2 Detection is a one-step / one-solution, biotin-free detection system which combines superior work flow and sensitivity for mouse and rabbit primary antibodies.

MACH 2 detection consists of a single reagent applied after the primary antibody. MACH 2 is ideal for use with prediluted antibodies or concentrates with equal success. MACH 2 may be used for 7-step multiplex stains with certain antibodies.

MACH 2 increases staining specificity compared to conventional dextran backbone polymer. MACH 2 is available in anti-mouse, anti-rabbit, and 'universal' (anti-mouse & anti-rabbit) formulations, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP).

  • Increase specificity 3-4 fold in nuclear staining
  • Increase specificity 2 fold in cytoplasmic/cell surface staining
  • Superior to dextran backbone polymers

MACH 3™

MACH 3 is a two-step, biotin-free detection system which provides excellent specificity, sensitivity and nuclear staining for mouse or rabbit primary antibodies.

MACH 3 Features:

  • A sensitivity-enhancing secondary reagent
  • Increased density of enzymes bound to tertiary target
  • 5- to 10- fold increased staining sensitivity when compared to conventional dextran polymer detection kits
  • Superior staining for nuclear and cytoplasmic/cell surface antigens
  • Elimination of background
  • High primary antibody dilution significantly improves specificity and reduces cost
  • Adapted for all Biocare's prediluted antibodies
  • Compatible with automated immunostainers

MACH 4™

MACH 4 is a highly sensitive universal detection system. The MACH 4 system can detect either mouse or rabbit antibodies, and is a two-step universal detection method.

MACH 4 micro-polymer system incorporates two key technologies:

Enhanced probe to increase sensitivity 10- to 20- foldIncreased density of enzymes bound to tertiaries

MACH 4 Features:

  • 10 - 20 times more sensitive than conventional dextran polymer systems
  • 20 - 40 times more sensitive for nuclear staining
  • Suitable to detect mouse specific anti-biotin or antidigoxigenin antibody in in situ hybridization (ISH)
  • Micro-polymer allows superior specificity/sensitivity, nuclear access and primary antibody dilution
  • Compatible with automated immunostainers

Multiplex

Biocare Medical is the proven leader in providing multiplex detection platforms that enable simultaneous IHC staining with multiple antibodies on a single slide. This superior detection system increases reimbursement per slide, simplifies procedures and reduces turnaround time.

Multiplex Micro-polymer Detection Kits

The innovative AP (Alkaline Phosphatase) and HRP (Horseradish Peroxidase) micro-polymer technology is specially designed for a rapid multiplex stain procedure, i.e. multiple antigens on the same slide. The micro-polymer confers significant increase in staining sensitivity when compared to conventional polymer detection systems. The micro-polymer multiplex detection kits are optimized for human tissue.

Key features of multiplex micro-polymer detection:

  • Simplifies protocols by reducing the number of steps (4 steps)

  • Eliminates endogenous biotin blocking

  • Process >50 percent fewer slides

  • Results in less than two hours

  • Compatible with automated IHC instruments

  • Significant increase in staining sensitivity

PromARK™

Biocare's PromARK micro-polymer detection systems are designed specifically for use on animal tissues. The micro-polymer technology provides superior sensitivity and minimum cross-reactivity to endogenous mouse and rat IgG. The Promark product line includes specially formulated heat-retrieval solutions, blocking agents, and one- and two-step detection reagents.

  • High staining sensitivity and specificity
  • Minimum cross-reactivity to endogenous IgG with proprietary blocking reagents
  • Elimination of endogenous biotin
  • Reduced number of IHC steps
  • Use with formalin-fixed, paraffin-embedded tissues, floating sections, frozen sections and cell culture preparations
  • Highly effective ancillary reagents
  • Suitable for both manual and automated techniques

Animal tissues contain endogenous immunoglobulins that produce significantly high levels of background staining when standard anti-mouse/anti-rabbit detection systems are employed. Biocare provides micro-polymer detection systems for use with primary antibodies from various sources (mouse, rat, rabbit, goat) on a range of tissues (mouse, rat, canine, feline, bovine, equine, porcine, ovine) which result in reduced background staining and higher sensitivity.

Starr Trek™

Available for use with HRP or AP, these highly cost-effective kits are compatible with Biocare prediluted antibodies. Using a streptavidin AP or HRP reduces non-specific background staining. Starr Trek is much more stable than the ABC complex.

Kits are color-coded for ease of use. Starr Trek kits come with Universal Link and avidin-enzyme reagents. We recommend Betazoid DAB for optimal performance with the HRP Kit.

About Biocare Medical

Biocare Medical, LLC is an innovator in developing and supplying world class automated immunohistochemistry instrumentation, and the full range of reagents for IHC lab testing. Biocare is the market leader in simultaneous Multiplex IHC tests which provide increased confidence at critical diagnostic decision points, impacting patient therapy while accelerating turnaround time. Biocare's advanced micro-polymer™ detection enables unsurpassed specificity and sensitivity. PROMARK™ and "mouse on mouse" IHC detection kits are optimized for rat and mouse tissues and feature proprietary blocking and retrieval reagents, resulting in virtually background free IHC staining.

The company's customers include clinical histology laboratories, pharmaceutical companies, CROs, and biotechnology companies as well as academic, government, military, and other non-profit laboratories. Biocare Medical offers an expanding portfolio of integrated products to address the rapidly growing cancer and infectious disease diagnostic and research markets with novel tissue immunohistochemistry and in situ hybridization methods. Biocare medical is headquartered and has manufacturing facilities in Concord, Calif., and has a global distribution network.

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