Amnis

Integrating flow cytometry and

microscopy to advance discovery

ImageStreamX Mark II

High Resolution Microscopy in Flow

The ImageStreamX Mark II combines the speed, statistical power, and fluorescence sensitivity of flow cytometry with the functional insights of highresolution microscopy to give you the most insightful cell analysis possible.

The Mark II includes a host of improvements to accelerate discovery:

Fast: Acquire More Cells
The ImageStream®X Mark II Imaging Flow Cytometer can analyze up to 5,000 cells/sec with real-time intensity compensation - ideal for rare cell analysis.

Easy to Use: Increased Power, Less Complicated
The intuitive user interface provides real-time plotting and graphical gating, as well as images of every cell. The easy-to-use compensation wizard quickly guides you through setup of multi-color compensation matrices.

Flexible: Fits Your Sample, Your Targets, Your Fluorophores
The ImageStream®XMark II Imaging Flow Cytometer accepts up to 7 lasers and works with sample volumes of 20-200 ul for added experimental flexibility - perfect for multi-user laboratories.

Efficient: Less Sample Volume, Less Sample Waste
Fluidics that provide up to 95% sample utilization when every cell is required. Recover unused sample for further analysis when orthogonal methods are required.

Any Upgrade, Any Time
The modular design of the ImageStream®X Mark II Imaging Flow Cytometer allows the system to grow with your research needs and budget through a broad range of field-installable options. Most options, including additional lasers, can be installed at the factory or upgraded in the field by Merck Millipore-certified field service personnel.

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MODULAR OPTIONS


7 Lasers

The standard 488 nm laser of the ImageStream®X Mark II Imaging Flow Cytometer may be augmented with up to six additional lasers, including 375, 405, 561, 592, and 642 nm. A high-power 488 nm laser upgrade is also available for enhanced detection of fluorescent dyes excited at that wavelength.


12 Image Channels

Up to 12 channels of detection are available with the addition of an optional second camera system and associated optics. The additional channels vastly expand the scope of potential phenotypic and functional analyses.


MultiMag

The MultiMag option provides 20X and 60X objective lenses in addition to the standard 40X objective for greater experimental flexibility and improved resolution. The 60X objective increases magnification for small objects such as yeast and bacteria and offers greater detail with mammalian and plant cells. The 20X objective provides an increased field of view for the imaging of large cells, such as epithelial cells.


AutoSampler

The new AutoSampler option enhances productivity with unattended sample loading from multiwell plates. The AutoSampler allows you to easily perform dose response and time course studies.


Extended Depth of Field

EDF™ extended depth of field technology uses a combination of specialized optics and unique image processing algorithms to project all structures within the cell into one crisp plane of focus. Applications that require the enumeration of spots within the cell or nucleus, like fluorescence in situ hybridization (FISH), require an increased depth of field. EDF makes it possible to find and count spots anywhere within the cell without any sacrifice in sensitivity or speed.


Full Color Brightfield

The Full Color Brightfield option provides a full spectrum brightfield light source that allows the ImageStreamx system to replicate the RGB brightfield imagery of a microscope. This option allows brightfield to be assigned to any channel not used for flourescence, increasing experimental flexibility.


APPLICATIONS


Cell Signaling and Molecular Translocation

Measure translocation of transcription factors or membrane proteins.

Internalisation

Measure the internalization of proteins or particles from the cell surface to the interior.

Morphology Analysis

Measure cellular and nuclear morphology to quantify shape change.

Co-localization

Measure molecular trafficking to organelles or the co-localization of cell surface or nuclear proteins.

Cell-Cell Interaction

Measure the accumulation of protein at the immune synapse or the inter-cellular boundary during virus-induced cell fusion.

Cell Cycle and Mitosis

Enhance cell cycle analysis with a mitotic index derived from nuclear morphology.

DNA Damage and Repair

Rapidly and objectively measure the characteristic nuclear staining of DNA repair proteins.

Cell Death and Autophagy

Quantify DNA fragmentation, the hallmark of apoptosis, and quantify LC3 clustering, the hallmark of autophagy.

Spot Counting

Quantify the number of discrete spots in images.


DISCIPLINES


The ImageStream imaging flow cytometer combines the visual power of microscopy with the speed and sensitivity of flow cytometry by digitally imaging cells directly in flow. Reveal biological phenomena using the brightness and location of fluorescence on, in, and between cells - even within rare sub-populations. Here you will find a sampling of some of the applications organized by discipline.

Biochemistry

The ImageStream provides the biochemist with the ability to measure nuclear translocation, co-localization of probes, or trafficking between organelles even in rare sub-populations.

Immunology

The ImageStream offers the immunologist the image resolution and throughput necessary to measure phagocytosis, immune synapse formation, or shape change in response to cytokines.

Oceanography

The ImageStream's tremendous speed and resolution are unmatched by any other imaging platform for oceanography.

Oncology

The ImageStream's ability to image cells directly in suspension make it a natural fit for leukemia and lymphoma research and, ultimately, clinical use. Regardless of what kind of cells you study, the ImageStream can characterize the N/C ratio, produce an accurate mitotic index, measure signal transduction, follow the uptake of drugs into cells, and measure apoptosis, all at the same time.

Haematology

The ImageStream allows the characterization of cells based both on phenotype and on nuclear and cellular morphology, texture, and other morphologic characteristics. This capability is extremely valuable for the study of hematopoiesis and leukemia/lymphoma.

Stem Cell Biology

The ImageStream has been used extensively for stem cell studies, thanks to its ability to characterize cells using both morphologic and phenotypic parameters.

Drug Discovery

With the ImageStream one can automatically sample large numbers of cells for high content analysis to screen multiple drug candidates.


The FlowSight

Flow Cytometry with Vision

Capable: Sensitive and flexible for every need

The FlowSight offers high performance in a small package. Its innovative design increases signal and minimizes noise to provide unmatched fluorescence sensitivity. Twelve standard detection channels simultaneously produce brightfield, darkfield and up to ten channels of fluorescence imagery of every cell. With these unique capabilities, the FlowSight enables a broad range of applications.

Amnis FlowSight - 12 channel capability

Intuitive: Easy-to-use, with imagery for every cell

The FlowSight operates like a conventional flow cytometer but also provides imagery of every cell. Powerful and intuitive analysis software seamlessly links quantitative data to imagery:

  • Click on a dot in any plot to see the corresponding cell imagery.
  • Click on a bin in any histogram to view all the cells in that bin.
  • Draw gates on dot plots and view the resulting populations to validate results.

Amnis FlowSight - Easy to use

Affordable: Designed and priced for every lab

The FlowSight is powerful enough for the core lab but sized and priced for any lab. The system can be factory configured or field upgraded with up to four excitation lasers (405, 488, 561, 642 nm), a 96-well plate AutoSampler, and a powerful quantitative image processing option. Whether in a base configuration or fully-optioned, the FlowSight sets a new standard of value.

Amnis FlowSight - up to 4 lasers


About Amnis

Amnis designs, manufactures and markets leading cell imaging instrumentation for the research and clinical markets. The Company's flagship product is the ImageStream system, which combines the speed and sensitivity of flow cytometry with the power of high resolution digital microscopy. As the only technology capable of imaging cells at high speed directly in bodily fluids, ImageStream enables unique, non-invasive diagnostic applications. These applications include residual disease detection via high throughput FISH, circulating endothelial cell and tumor cell analysis, and advanced automated hematology based on multi-color phenotyping combined with histochemical staining.

Amnis was founded in 1999 and has generated ~50% annualized revenue growth since inception. The Company holds 32 issued US patents and has 55 pending patents covering applications, subsystems, and the ImageStream architecture for imaging both in flow and on substrates. Amnis is privately held and benefits from a strong investor group that includes MedVenture Associates, Crown Venture Fund, and OrbiMed Capital.

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