Cellular Immunity and Oncology Flow Cytometry Reagents

eBioscience, now an Affymetrix company, is a world leader in immunology and oncology flow cytometry reagents for both Life Science research and diagnostics.

The eBioscience product line contains a comprehensive and innovative portfolio of fluorochrome conjugated antibodies and reagents for multicolor flow cytometry. In addition to multicolor flow cytometry reagents, eBioscience offers highly-validated ELISA kits, recombinant proteins and general-purpose lab reagents for complete biological system analysis.

eFluor® Fluorophores and Dyes

eFluor is the eBioscience brand of fluorophores and dyes developed specifically for labeling and detection of biomolecules. With the eFluor brand you will find both nanocrystal and organic dye fluorophores for cell analysis, as well as functional dyes that enable cell proliferation and cell viability assays. Each eFluor fluorophore is engineered for superior optical performance in a variety of applications.

eVolve™ QDots®
High quantum yield QDots® possess a heavy metal core particle surrounded by a surface coating enabling use in a wide variety of applications.

eFluor® Organic Dyes
Traditional fluorescent dyes for use in a wide variety of applications.

eFluor® Functional Dyes
Dyes optimized to enable cell proliferation assays and tracking cell viability.

Clinical Diagnostic Reagents - Optimized for today's clinical flow cytometry environment

Leading Edge, Established Clones
Rapidly expanding portfolio of clinically relevant products.

Optimized Format
Antibodies are packaged with 20% more product than the leading competitor at a price that's about 20% less. Universally formulated at 5µL/test to make multicolor work efficient.

Conjugation Flexibility
Fluorochrome options among most specificities; compatible with all flow cytometers. An extensive portfolio of leading edge cell analysis products and technologies position our customers at the forefront of multicolor flow cytometry.

Outstanding Signal : Noise
High quality reagents manufactured to the highest standards. Outstanding brightness and greater than 90% purity. Reagents that consistently provide optimal signal with low background and reproducible lot-to-lot performance save you both time and money.

Uncompromising Quality
Manufactured to industry-leading tolerances for optimal signal with low background.

Lot-to-Lot Consistency
Consistent performance between production lots is a requirement of the eBioscience manufacturing specifications. In addition to optimal performance, reagents from eBioscience demonstrate dependable lot-to-lot consistency. This commitment to quality ensures reliable data from long-term studies and eliminates the need to recalibrate your staining for a new lot of antibody. Our stringent manufacturing specifications require consistent performance between production lots to ensure reliable and accurate results in your studies.

Established Reputation
Scientists have long recognized that eBioscience reagents represent an outstanding value, resulting in reagents being cited in over 20,000 publications in the past 10 years. Now, through an exclusive partnership with The Binding Site, a rapidly expanding portfolio of clinical reagents is available, designed for the needs of today's clinical flow cytometry lab.

ELISA - Platforms tailored to meet your needs

ELISAs (Enzyme-linked immunosorbent assays) are routinely used for the qualitative and quantitative assessment of cytokines, chemokines, growth factors, phosphorylated targets, immunoglobulins and other immunological markers. Detection and quantitation of protein analytes from various biological samples-serum, plasma, cell culture supernatants and cell lysates-indicate a multitude of biological and pathological events. ELISAs have proven to be valuable tools in drug development as they are commonly used for biomarker profiling of disease vs. normal states, as well as toxicity profiling.

eBioscience ELISAs use the sandwich immunoassay technique of two highly specific antibodies that recognize two different epitopes on the same protein. This provides an extra layer of specificity over competitive ELISA formats that employ only the use of a single antibody.

Pre-coated ELISA - Platinum ELISA
Quantitate with confidence. Highly validated, pre-coated ELISA kits provide lower inter- and intra-assay variability with ready-to-use reagents, ensuring consistent data throughout your research.

Coat-It-Yourself ELISA - Ready-SET-Go!® ELISA
Keep costs low. Each affordable, coat-it-yourself ELISA set includes the reagents required to prepare and run the ELISA.

1-wash ELISA - Instant ELISA®
Reduce steps and hands-on time to increase productivity. The 1-wash ELISA introduces fewer handling steps, leaving more time for your research.

High Sensitivity ELISA
Detect low-abundant cytokines. Amplified sandwich ELISAs allow for detection limits below 1 pg/mL without loss of resolution or increase in background.

Analyze individual, activated, cytokine-producing cells. This reagent set is pre-titrated for optimal spot development.

InstantOne (phospho) ELISA
Save time and reduce steps. Our 1-hour, 1-wash cell signaling assay detects total and phosphorylated targets with sensitivity exceeding industry standards.

ProcartaPlex™ Multiplex Immunoassays

Multiplex immunoassays for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins, has proven to be an invaluable tool for the comprehensive study of biological systems.

ProcartaPlex Multiplex Immunoassays use Luminex® xMAP (multi-analyte profiling) technology that enables:

  • Quantitate up to 100 analytes (50 analytes for MAGPIX®) simultaneously
  • Use significantly less sample (only 25-50 uL)
  • Same amount of time that it takes to perform an ELISA

ProcartaPlex Multiplex Immunoassays are available in multiple formats across six species (Human, Mouse, Rat, Non-Human Primate, Porcine and Canine) to meet the needs of your research.

PrimeFlow™ RNA Assay - Enter a new dimension of single-cell analysis

PrimeFlow™ RNA Assay reveals the dynamics of RNA and protein expression within individual cells, facilitating unprecedented analysis of their correlation as the cell changes over time or in response to stimulus. This novel assay employs a proprietary fluorescent in situ hybridization (FISH) technique enabling simultaneous detection of up to three RNA transcripts in a single cell using a standard flow cytometer. RNA detection may be teamed together with intracellular and cell surface antibody staining to elevate the understanding of single cell dynamics to a new dimension.

Development of the assay is based upon proven and well-published Affymetrix® ViewRNA™ FISH assays designed for microscopic analysis of RNA in cells and tissues. The assay combines paired oligonucleotide probe design with branched DNA (bDNA) signal amplification to robustly detect gene expression at the single-cell level.

Coupling RNA expression with protein detection on a flow cytometer generates multiparametric data in heterogeneous cell populations and offers in-depth and high content details at the single cell level. In contrast, microarrays and sequencing can provide comprehensive gene expression data in bulk sample preparations; however, such bulk sample preps can mask the individual effects of different cellular subsets. Using the PrimeFlow™ RNA Assay, specific cell populations may be analyzed for unique transcript expression levels or cell subsets evaluated over time to determine transcriptional regulation and protein expression simultaneously. Such unique and valuable insights are highly applicable to answering previously unanswerable questions and have broad implications for advancing research across multiple fields of biology.

  • Observe gene expression heterogeneity at the single cell level
  • Compare kinetics of both RNA and protein expression in the same cell
  • Detect non-coding RNA in cellular subsets
  • Evaluate viral RNA in infected cells
  • Analyze mRNA expression levels when antibody selection is limited

QuantiGene® Assays

QuantiGene is a signal amplification technology utilized for the quantitation of gene expression transcripts. We have 4 different technologies that incorporate the branched DNA technology which is used to amplify the signal and provides a more robust and accurate measurement compared to qPCR. The QuantiGene Plex and QuantiGene Singlelex assays are designed to quantitatively measure RNA directly from cell or tissue homogenates without RNA purification. PrimeFlow™ and ViewRNA are in situ based assays that can measure or visualize RNA directly in the cells.

Flow Cytometry

Flow cytometry is a technique used to analyze multiple phenotypic and functional parameters simultaneously within a single cell or a population of cells. Technical advances in instrumentation, and an expanding array of fluorochrome choices, allow the design of multicolor staining panels that maximize the amount of information acquired from limited sample material. Realizing the power of multicolor flow cytometry requires high performance fluorochromes paired with appropriate specificities to ensure consistent and reliable data.

Functional Activity Products

An intrinsic part of evaluating any biological system, is the ability to mimic and/or inhibit relevent pathways, both at the cellular level and in tissue and whole organism models. The use of commercially available antibodies and recombinant proteins is crucial to life science researchers in every field and has advanced our ability to explore these systems with consistent, reproducible results. With these tools, biologic systems can be systematically examined in all aspects: from the initiation of cell signaling via receptor-ligand interactions to the induction of intracellular pathways that promote cell activation and differentiation, as well as the expression of unique phenotypes and protein expression patterns. eBioscience offers an expansive list of highest quality Recombinant Proteins and Functional Grade Antibodies designed for optimal results when used in activation, neutralization, or blocking studies.

Immunohistochemistry and Immunocytochemistry

Microscopy is the most widely used imaging method to explore biological systems, in which immunocytochemistry (ICC), the localization of proteins within cells, and immunohistochemistry (IHC), which reveals the abundance, distribution, and localization of biomarkers within a tissue, can be viewed directly. These techniques provide insight into cellular structure and mechanisms and are applicable for basic research, in addition to being indispensable in clinical settings. Target antigens may be evaluated using specific antibodies directly conjugated with an enzyme or fluorophores, or indirectly using similarly labeled secondary antibodies and reagents.

Immunoprecipitation and Western Blot

Immunoprecipitation (IP)
Immunoprecipitation (IP) is an enrichment procedure of specific proteins, protein modifications, or protein motifs from a heterogeneous protein sample such as cell lysate. The enrichment occurs through the use of an antibody that commonly recognizes a protein in its native or semi-native format. IP is often followed by a western blot for analysis, but can be followed by other applications as well. IP allows for the detection of proteins of low abundance, protein:protein interactions and protein post-translational modifications (PTMs).

Western Blot (WB)
Western Blot (WB), also referred to as immunoblot, is a common, effective method for determining relative protein or protein-modification levels in a sample. Denatured proteins are separated via SDS-PAGE and transferred to a membrane where the proteins are detected via the use of antibodies. The relative intensity, and location of the protein on the blot, allow for its identification and quantification.

Recombinant Proteins

Quality proteins for functional and bioassays, as well as for cell culture maintenance, proliferation and/or cell differentiation. Over 300 recombinant proteins available in multiple formats, including carrier-free options that surpass the industry-standard endotoxin specifications for in vivo/in vitro applications. Specialty proteins available for Stem Cell and Tumor Biology.

Contact Millennium Science today to learn more about
eBioscience products and to place your order.

About eBiosceince - and Affymetrix company

eBioscience provides innovative high quality reagents to researchers worldwide that empower the process of scientific discovery in the areas of cellular immunity and oncology.

Our extensive portfolio of leading edge cell analysis products and technologies focused on flow cytometry and immunodetection position our customers to be at the forefront of science. eBioscience is aligned with, and responsive to, our customers' ever-changing needs making eBioscience a preferred discovery partner.

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