ArcherDX

Archer™ NGS assays are purpose-built to identify mutations and gene fusions from clinical sample types. By combining novel enrichment chemistry, automated informatics, and a user-friendly workflow, Archer products remove the bottlenecks associated with using next-gen sequencing in a translational setting.

ArcherDX Technology

Archer™ FusionPlex™ Assays

Panel Types

Archer Workflow

Archer™ VariantPlex™ Assays

Contact

Archer Analysis

How does it work?

ArcherDX Technology

ArcherDX is a modular assay format comprising three components making this a powerful gene fusion detection system :

Robust platform enriches targeted RNA and DNA to detect known and unknown regions by NGS

Single-use, lyophilized reagents elimate master mix preparation, reducing hands-on time and potential for user error

Multiple levels of reporting and comprehensive results with single-base resolution

How ArcherDX stacks up against the rest:

Anchored Multiplex PCR (AMP)

AMP is a robust platform to create target-enriched NGS libraries that excels over traditional PCR-based preparation methods. Unidirectional gene-specific primers (GSPs) enrich for both known and unknown mutations. Adaptars that contain both molecular barcodes and sample indexes permit quantitative multiplex data analysis, read deduplication and accurate mutation calling.

Detect gene fusions with the
Archer FusionPlex™ System

Detect indels, SNPs and CNVs
with the Archer VariantPlex™ System

Archer Workflow

Modular Assay Format

RNA Workflow

DNA Workflow

Highlights:

  • Idenitify known and novel fusions and splice variants in a single assay
  • Compatible with 20ng input RNA from FFPE
  • PreSeq ™ QC Assay indentifies low-quality samples prior to librabry prep

Highlights:

  • Identify SNVs, indels and CNVs in a single assay
  • Compatible with 10ng input DNA from FFPE
  • Molecular barcodes and unique start sites support mutation calling and quantitative data analysis
  • Integrated fragmentation eliminates need for mechanical shearing

Archer Analysis

Automated, not complicated

  • Simple, visual tiered reporting - Top-level summary reports fusions, isoforms and variants as well as quality control status. Icons represent QC metrics.
  • Fusion verification - Fusions are cross-referenced with the Archer Quiver™ fusion database to indicate known or novel fusions
  • Variant summary - Fusion candidates, variants, predicted biological effects and the number of supporting reads are reported
  • Read and annotation visualisation - Fusions and reads can be viewed directly in Analysis Pipeline
  • Comprehensive Analysis Data - Coverage information for each target and quality metrics help determine the quality of the library sequenced and the integrity of the assay


Archer™ FusionPlex™ Assays

The Archer™ FusionPlex™ Assay system generates target-enriched cDNA libraries for next-generation sequencing (NGS). The system leverages Anchored Multiplex PCR (AMP™) to selectively amplify cDNAs of specific genes of interest in a sample along with any fusion partners, known or unknown.

The libraries are ready for single sample or multiplex sequencing on Illumina® or Ion Torrent™ next-generation sequencing platforms. The sequencing data is then analyzed using the Archer Analysis Pipeline to detect true positive calls for gene fusions (both known and unknown), isoforms, insertions/deletions (indels) and copy number variations.

And the FusionPlex system can do this with as little as 20ng input nucleic acid from FFPE samples.

The system is modular and requires one of each of the following:

  • FusionPlex Assay - a panel of target-specific primers
  • Molecular Barcode (MBC) Adapters - platform-specific half-functional universal adapters
  • Universal RNA Kit - platform-specific enzymes and reagents

KEY FEATURES OF ARCHER FUSIONPLEX SYSTEM

  • No prior knowledge of fusion partners and breakpoints required
  • Detect known and novel gene fusions from as little as 20ng input nucleic acid from FFPE samples.
  • Objective sequence-based data compared to subjective FISH analysis
  • Novel enrichment chemistry yields high on-target percentage
  • Random start sites improve sequence data quality

Archer™ VariantPlex™ Assays

The Archer™ VariantPlex™ system utilises Anchored Multiplex PCR chemistry to create genomic NGS libraries from low sample input. The system also uses single-step fragmentation that eliminates the need to shear genomic DNA. Lyophilized, pre-aliquoted reagents replace master mixes for greater consistency and the simple workflow reduces sample handling and the risk of pipetting errors.

KEY FEATURES OF ARCHER VARIANTPLEX SYSTEM

  • Full exon coverage
  • Integrated fragmentation
  • Random start sites for complex library preparation
  • Molecular barcodes for deduplication and confident mutation calling

FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

How does it work?


Below are the Archer FusionPlex and VariantPlex panels for RNA and DNA analysis

including the range of MBC adapators and Universal RNA kits for the RNA/DNA panels:

Panels

MBC Adapters

Universal RNA Reagent Kits

  • Illumina® MBC Adapters
  • Illumina® MBC Adapters, Set B
  • Illumina® MBC Adapters, Set C
  • Ion Torrent™ MBC Adapters
  • Illumina® Universal RNA Reagent Kit
  • Ion Torrent™ Universal RNA Reagent Kit

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