Next-Generation Sequencing

From Samples To Publication, Millennium Science Enables Your Next-Generation Sequencing Workflow

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We offer a range of products for use at select stages of the NGS workflow which are compatible across all Next-Generation Sequencing platforms.

SAMPLE PREPARATION - Cell Lysis and RNA Extraction

The NuGEN PreludeTM Direct Lysis Module provides a fast and easy method for whole transcriptome analysis using direct cell lysates without RNA isolation. Cell washing and lysis takes less than five minutes and the resulting lysates can be added directly to downstream NGS reactions.

Prelude™ Direct Lysis Module

• Traditional RNA extraction and isolation procedures are eliminated
• Simple assay protocol allows for RNA amplification directly from the Prelude
• Direct Lysis Buffer saving time and money
• Can be used with as few as 50 cells or as many as 10,000 cells.
• Assay flexibility to cater for your throughput needs
• Samples can be assayed directly from cell lysate without the need for further purification or can be frozen for later use
• High performance data: microarray and NGS expression profiling demonstrates highly concordant results of greater than 95% between cell lysate and isolated total RNA

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cDNA SYNTHESIS AND AMPLIFICATION

The OvationTM RNA-Seq System provides a fast and simple method for preparing amplified cDNA from total RNA for RNA-Seq applications while the Ovation 3'-DGE System provides accurate expression analysis (tag profiling) suitable for quantification of any poly(A)+ transcripts, without the need for prior sequence knowledge.

Ovation® RNA-Seq System

• Allows for transcriptome analysis of small amounts of RNA
• Analysis of both poly and nonpolyadenylated transcripts
• Double-stranded cDNA product is optimized for library construction
• Only 500pg of starting material is required which produces sufficientcDNA for several NGS runs
• Assay takes only 6-hours total time
• No rRNA reduction or poly(A) selection is required

Ovation® 3'-DGE System

• Compatible with all NGS platforms
• Flexible input - use as little as 10ng
• of total RNA or product of the Prelude Direct Lysis system from as few as 50 cells
• 9 hours total assay time
• Prior sequence knowledge is not required for expression profiling
• Increase power & reduce costs with the optional indexing capability of the NuGEN EncoreTM NGS Library System

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TARGET CAPTURE AND ENRICHMENT

Fluidigm's Access ArrayTM System delivers the throughput and ease-of-use required to get the most out of your next generation sequencer. The Access Array is an open platform comprised of a microfluidic chip that allows amplification of 48 specific amplicons from 48 unique samples, in effect preparing 48 libraries in just a few hours.

Access Array System

• Sample Capture and Target Enrichment - Only sequence areas of interest
• Sample Barcoding for Multiplexed Sequencing - Sequence multiple samples per run
• Sequencing Library Preparation Using Amplicon Tagging - Eliminate traditional library preparation
• High Throughput - Enriched targets from 48 samples per chip
• Easy-to-Use - Only six manual steps required to produce 2,304 amplicons
• Time efficient - Prepares 48 libraries in a few hours at minimal cost

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AUTOMATED SIZE FRACTIONATION

The LabChip® XT from Caliper Life Sciences delivers reproducible, tightly sized samples in a sequencing buffer using microfluidics. The XT improves laboratory efficiency by performing automated nucleic acid fractionation accurately and reproducibly and provides sizing that is difficult to obtain using manual methods.

LabChip® XT

• Fast, reproducible, high resolution size selection
• Less than 30 minute processing
• Up to 4 samples processed simultaneously per chip
• Quantitative, digital data
• Sample tracking via barcode
• Completely independent channels minimizes potential for cross-contamination
• No post-purification, with collection in PCR-compatible buffer

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LIBRARY CONSTRUCTION

For library construction on the Illumina NGS System, the EncoreTM NGS Library Systems from NuGEN has been developed for seamless integration with both the Ovation® RNA-Seq System and Ovation® 3'-DGE System to enable a complete end-to-end solution for transcriptome library construction using total RNA samples in as little as 9 hours.

EncoreTM NGS Library System I & EncoreTM NGS Library Multiplex System I

• Library construction in as little as three hours, with only two purification steps and no gel purification required
• All required components are included to make libraries for RNASeq, genomic DNA sequencing, ChIP-Seq or Digital Gene Expression applications on Illumina NGS platforms
• Optional indexing capability for multiplex sequencing to increase sample throughput and reduce sequencing costs
• Seamless integration with NuGEN's Ovation RNA-Seq and 3'-DGE Systems
• Compatible with the Illumina NGS sequencing platforms

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DATA ANALYSIS AND BIOLOGICAL INTERPRETATION

NextGENe® from SoftGenetics is unique biologist-friendly software that analyses the vast amount of data generated by Next-Generation Sequencing. Its simplicity, accuracy, speed and powerful algorithms backed by technical support from Millennium Science and SoftGenetics all but eliminates the need for additional bioinformatics resources.

NextGENe from SoftGenetics

• Easy Gene Annotation and Simple navigation
• Rapid review of variants, nucleotides and amino acids
• Flexible mutation scoring system makes analysis of large projects faster and easier
• Up to ten projects utilized with the same reference can be compared at one time
• Quick links to NCBI & dbSNP
• Easy exporting of results
• Over 15 applications including the Condensation Tool, SNP & INDEL Detection, ChiPSeq Analysis, miRNA Discovery, Quantification, Transcriptome Analysis and much more
• Compatible with all new NGS platforms including the Applied BioSystems SOLiD™ System, Roche Genome Sequencer FLX™ and Illumina Genome® Analyzer

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Take your NGS data analysis results to the next level with Ingenuity Pathway Analysis (IPA ) software. IPA helps researchers model, analyze, and understand the complex biological and chemical systems at the core of life science research. IPA has been broadly adopted by the life science research community and cited in hundreds of peer-reviewed journal articles.

Biological Interpretation with IPA

• Find information about genes, drugs, biomarkers, chemicals, cellular and disease processes, and signaling and metabolic pathways
• Build dynamic pathway models to quickly and easily model and analyze experimental data
• Unlock the insights buried in experimental data by identifying relationships, mechanisms, functions, and pathways of relevance
• Share customized pathways and analysis results with internal and external collaborators through interactive emails, lists, analysis summaries, and pathways
• Publish research with the support of the most frequently cited pathway solution in the scientific peer-reviewed literature

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