Sage Science

Millennium Science now offer Sage Science Pippin™ Automated DNA size selection and whole-sample fractionation of DNA or protein providing an invaluable tool for geneticists when preparing libraries for next-gen sequencing (NGS).

The Technology

Targeted DNA Size Selection: Pippin

Pippin's disposable gel cassettes are precast with agarose. DNA sizing is determined by the detection of markers in a dedicated lane or internally in the sample lane and using those to select and collect the desired fragments based on user-input values.

Each sample lane is physically separate to eliminate the possibility of sample cross-contamination and features a branched configuration with three electrodes. DNA is separated along a gel column until the programmed fragment range reaches the branch point. The instrument then switches the active electrode to divert DNA into a membrane-bound buffer chamber. When the size range has been collected, the active electrode is switched back to the separation channel. The desired sample can be removed with a standard pipette.

Watch how it works:



The range of products available for Pippin:


PippinHT

High Throughput Sizing for Next-Gen Sequencing

90bp up to 1.5kb

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BluePippin

Targeted Size Selection for Next-Gen Sequencing

(90bp - 50kb)

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PippinPrep

Targeted Size Selection for Next-Gen Sequencing

(90bp - 1.5kb)

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PippinPulse

Pulsed-field Power for Gel Boxes

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Key Downstream Technologies

  • whole genome sequencing
  • miRNA library isolation
  • Mate-pair sequencing
  • Single molecule sequencing
  • Protein mass spec
  • Paired-end sequencing
  • emPCR
  • RNA-seq
  • ChIP-seq

Download the Pippin family brochure and the Pippin Pulse flyer


Whole Sample Fractionation of DNA or Protein: SageELF

The fully automated SageELF (for Electrophoretic Lateral Fractionator) can be used to select DNA or proteins. It features a cassette system designed to fractionate a whole protein or DNA sample simultaneously into 12 contiguous size fractions.

For proteomics users, SageELF delivers 12 size fractions in SDS buffer ready for analysis or identification on a mass spectrometer.

For scientists working with DNA, the generation of 12 contiguous fractions maximizes the utility of precious samples and can be used to build multiple libraries of different insert sizes for both short-read sequencers and long-range technologies that can assess structural information.

Watch how it works:

The range of products available for Whole Sample Fractionation:


SageELF

Whole Sample Fractionation

of DNA or Protein

Collect up to

12 fractions

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Key Downstream Technologies:

  • Proteomics and mass spec
  • Mate-pair sequencing
  • Splice variant detection

Download the SageELF brochure


To learn more about Pippin and SageELF products fill in your details below.

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