LI-COR Biosciences
Near Infra-red Imaging Technology
Protein • In-vivo • DNA
Millennium Science is the exclusive distributor for LI-COR Bioscience's Biotechnology product line including the range of the Odyssey family of near-infrared and chemi protein detection systems, the Pearl in vivo imaging systems and the 4300 DNA Analyzers.
With over 20 years of experience, LI-COR Biosciences is a leader in imaging technologies that expand analysis for genomic and proteomic applications. The technology behind LI-COR's innovative solutions is based on detection at near-infrared wavelengths. Researchers gain a competitive advantage with these technologies due to the low background and high sensitivity that can be achieved using the near-infrared spectral region. LI-COR's hardware designs include everything from laser-based excitation systems to proprietary CCD camera designs that have advanced noise reduction capabilities for peak sensitivity.
Infrared fluorophore design and production are also part of LI-COR's core competencies. Their patented IRDye® Infrared Dyes can be used to label nucleic acids, antibodies, proteins, peptides, viruses, cytokines, chemokines, and more. To remain ahead of advancing trends, new fluorophores and labeling methods are constantly in development.
Customers often remark that images produced from LI-COR products are more clear and informative. These results are an expression of the underlying technologies, which includes software that utilizes the latest techniques to visualize, analyze, and make data more understandable. Integrated solutions comprising hardware, software and labeling technologies have been responsible for many "firsts", including the first truly quantitative Western blots on the Odyssey Infrared Imaging System.
LI-COR's solutions are designed for high reliability and ease of use because they understand the impact that instrumentation problems and difficult lab procedures can have on your research.
The Standard for Western Blot Technology
As a researcher, your goal is to efficiently present the most accurate data possible. For more than 30 years, traditional chemiluminescent detection with fi lm has provided data that have been published by scientists worldwide.
Over the past decade, LI-COR® has revolutionized methods for protein detection that completely eliminate the need for film. Traditional chemiluminescent detection with film provides proven sensitivity, and LI-COR offers industry-leading technologies to maintain that sensitivity and improve your data quality to make it the clearest and most accurate it can be. LI-COR has used its expertise in optical design to provide methods for both chemiluminescent, as well as infrared fluorescence protein detection, without the use of film.
BENEFITS OF DIGITAL IMAGING
- Save valuable money on film
- Eliminate costs related to darkroom maintenance
- No need for multiple exposures
- A wide, linear dynamic range without saturation or "blown out" lanes
- Accurate detection of strong and weak bands in one exposure
- Sensitivity equal to or greater than that of film
- Eliminate the need for excessive washes and hazardous waste associated with film development
- Reduce the negative environmental impact related to film development
Whether you are looking to improve your Western blot data by simply moving to digital chemiluminescent detection or by transitioning to quantitative Western blot technology using infrared fl uorescent imaging, we will help you fi nd the best solution for you and your lab.
Odyssey CLx Infrared Imaging System - NEW
The Quantitative Western Blot Standard for Almost 10 Years Just Got Better!
The Odyssey CLx Infrared Imaging System is the next generation of the Odyssey Classic, the most trusted and established standard in Western blot technology.
The Odyssey CLx improves upon the Odyssey Classic through:
- Simplified User Interface
- Improved Dynamic Range
- Time-Saving Autoscan Feature
The Odyssey CLx is the most flexible and multifunctional platform of the Odyssey imaging systems. It has been designed to accommodate numerous applications, helping you to achieve results faster. The Odyssey CLx offers ease of use with one-button image acquisition as part of an autoscan feature that provides a wide dynamic range greater than six logs. This feature, along with a simplified user interface, allows you to reach excellent results simply and efficiently.
Two Independent Infrared Detection Channels
Two separate lasers and detectors simultaneously detect both fluorescent signals. The optical system employs diode lasers and solid-state detectors with long lifetimes and very low maintenance requirements. Infrared laser excitation outperforms systems that use white light, LED light sources, and filter wheels by delivering higher intensity excitation light to the fluorophore. A variety of fluorescent dyes and stains are compatible with the 700 nm and 800 nm excitation wavelengths of the two diode lasers in the Odyssey CLx. Spectral overlap is minimized by a 100 nm separation of the two detection channels, and optical filtering ensures that each detector measures fluorescence from only one of the infrared dyes.
CLx Applications
Western Blots: Two-Color Infrared and In-Gel
Cell-Based Assays: In-Cell Western™ and On-Cell Western
Protein Detection: Coomassie-Stained Gels, Membrane and Slide Arrays
Small Animal Imaging: In Vivo, Whole Organ, and Tissue Section
Nucleic Acid Detection: Mobility Shift Assays, DNA Gel Staining (Syto® 60), and Arrays
Microwell Assays: ELISA/FLISA, Protein Arrays, and RNAi Analysis
Odyssey Fc Imaging System
The Odyssey Fc Imager is the first CCD imager that offers excellent infrared (IR) fluorescent and chemiluminescent imaging performance in one system. The Odyssey Fc optical system combines solid-state laser diodes at 685 and 785 nm with a low-noise, CCD camera for detection of IR and chemiluminescent signals.
Chemiluminescence detection is simplified with the Odyssey Fc Imaging System. Film and darkroom steps are eliminated, along with the hazardous waste and excessive water usage of film development.
FieldBrite™ XT technology acquires images over the full dynamic range every time, with minimal user adjustments. With the proprietary technologies of the Odyssey Fc Imaging System, a single instrument can perform quantitative IR fluorescent detection PLUS qualitative chemiluminescent detection. Publication-quality images are easy to produce with either detection method. (Because chemiluminescence is a time-dependent enzymatic reaction, its quantitative range is limited. IR fluorescence is superior for quantification.)
The Odyssey Fc Imaging System includes LI-COR Image Studio Software for fast, easy image acquisition and analysis. At the push of a button, targets of interest can be imaged over more than six logs of linear dynamic range in every acquisition.
OVERVIEW AND ADVANTAGES
Wide Linear Dynamic Range
Accurately detect strong and weak signals over a broad linear dynamic range.
Two-Color Detection and Quantification
Detect two targets simultaneously on the same membrane increases the accuracy of quantification and comparison.
High Sensitivity
Near-infrared fluorescence provides sensitivity equal or better than chemiluminescence giving you low background, high signal-to-noise, multiplexed detection, and the sensitivity of any fluorescent system.
Image Studio Software
User-friendly software with easy navigation makes acquisition and analysis straight-forward.
Direct Detection for Better Data and a Cleaner Planet
No film or darkroom needed. IRDye signals on membranes are stable indefinitely if stored properly.
Flexible System with Many Applications
The Odyssey Fc system has been used for many applications, including Western blot analysis, EMSA, In-Gel Westerns, Coomassie gel documentation and DNA gel documentation.
Pearl Impulse
With the Pearl® Impulse, you can:
- Visualize imaging agents dynamically with real-time imaging
- Use native cells and cell lines for in vivo imaging, without reporter genes
- Achieve exceptional sensitivity and deep target imaging with laser excitation and near-infrared fluorescent detection
- Detect two near-infrared probes simultaneously
- Acquire optimized images quickly with one-button operation
- Use validated targeting agents to get started immediately without developing your own agents
OVERVIEW AND ADVANTAGES
Excellent Sensitivity
Laser-based excitation and near-infrared detection result in exceptional sensitivity, with deeper tissue penetration and earlier target detection.
Fast Imaging
Rapid, one-button acquisition of 700 nm, 800 nm, and white light images enables quick screening of animals and simultaneous detection of different targets.
Native Cell Compatibility
Using IRDye® targeting agent solutions for fast research startup - without reporter gene transfection or cell line development.
Enviromental Control
With HEPA-filtered imaging beds for immunocompromised animals, docking stations and anesthesia systems.
4300 DNA Analyzer
For the professional, student, or faculty researcher, the 4300 DNA Analyzer from LI-COR will accelerate your research and improve the quality of your data. High confidence in more precise data is the edge that researchers using LI-COR DNA analyzer have had for more than 20 years. The 4300 System is a third generation instrument based on LI-COR's highly sensitive infrared fluorescence detection technology.
OVERVIEW AND ADVANTAGES
Infrared Advantage
4300 instrumentation, software, reagents, and applications are all part of an integrated system designed to achieve the highest accuracy. Infrared detection is a key advantage since biological molecules, gels, and glass plates have little infrared fluorescence. With low background fluorescence, dye signals are clean and strong. Infrared detection gives 4300 DNA Analyzers excellent sensitivity.
Independent Detection
4300 DNA Analyzers feature LI-COR's unique, two channel detection that eliminates errors due to fluorescence overlap. The fluorescence of the two IR dyes are separated by 100 nm. Two separate sets of optically tuned lasers and detectors measure fluorescence from IR dyes independently.
High Sensitivity and Wide Dynamic Range
Underloaded or overloaded samples are accurately detected because LI-COR images use more than 65,000 grayscales per pixel (16-bit) to display the wide dynamic range of the 4300 DNA Analyzer. Unprocessed fluorescence data are captured in TIFF image format for highest fidelity.
Two Highly Reliable Diode Lasers
The 4300 DNA Analyzer is designed for multi-purpose operation. In addition to being an excellent research tool, the 4300 DNA Analyzer is designed to meet demanding undergraduate training and research environments. Features such as diode lasers (similar to a CD player) make the instrument rugged and dramatically reduce maintenance and operation expenses. The instrument can also operate on demand after periods of inactivity without any expensive start-up costs.
Two Separate Detectors
As samples pass in front of the scanning laser/microscope, two solid-state avalanche photodiodes detect fluorescence. Optical filtering assures that each detector measures fluorescence from only one of the infrared dyes.
Two Real Time Images
A separate image (similar to an autoradiogram) for each IR dye is collected in real time and can be displayed in an Internet browser or LI-COR application software (e-Seq, Saga, etc.).
Easily Adapts to any Lab or Network
The 4300 instrument contains a server that can operate either in a stand-alone configuration or connected to your network. An Internet browser or LI-COR Application Software can be used to start electrophoresis. The server software completely controls electrophoresis and data storage during a run. Two 16-bit TIFF images are stored on the 4300's server during a run. The 4300 System gives you maximum data access. Any computer can connect to the 4300 System via Internet browser or LI-COR Application Software (e-Seq, Saga, etc.). In a network environment, image data can be accessed from your office or over the Internet. Secure, password-protected server software assures that only members of your project have access to your data. The 4300 System is as expandable as the Internet. You can add all the capacity you'll ever need, without ever buying another computer. One computer can connect to any number of 4300 Systems to start runs or retrieve and analyze data.
About LI-COR Biosciences
Providing answers. From the extremes of the rain forests in South America to the harsh conditions of the Antarctic, and to cutting edge research laboratories around the globe, scientists rely on products from LI-COR Biosciences to provide answers to their questions.
LI-COR first introduced scientific instruments for plant science research and quickly grew to provide scientists tools for such diverse disciplines as atmospheric research and the study of how proteins interact at the cellular level.
LI-COR Biosciences is a global leader in the design, manufacture, and marketing of high quality, innovative instruments, software, reagents, and integrated systems for plant biology, biotechnology, drug discovery, and environmental research.
More than 30,000 customers in more than 100 countries use LI-COR instruments. In addition to its Lincoln, Nebraska headquarters, LI-COR has offices in Germany and the United Kingdom. The company also sells products through a global network of distributors.
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