Near Infra-red & Chemiluminescent Imaging Technology
Western blotting reagents • Western Blotting
Millennium Science is the exclusive distributor for LI-COR Bioscience's Biotechnology product line including the range of the Odyssey® family of near-infrared and chemiluminescent protein detection system, the Pearl® Impulse in vivo imaging system, and the C-DiGit® Chemiluminescent Western Blot Scanner.
We are also the exclusive Australian and New Zealand distributor of LI-COR IRDye secondary antibodies and related Western blotting reagents
About LI-COR Western blotting reagents
IRDye infrared fluorescent dyes and near-infrared (NIR) fluorescence imaging deliver enhanced sensitivity due to low background autofluorescence in the near-infrared region and, therefore, higher signal-to-noise ratios.
IRDye fluorescent dyes have absorption and emission wavelengths in the NIR spectrum, between 680 and 800 nm. Most of the IRDye reagents are compatible with the Odyssey® CLx, Odyssey Fc, Pearl Impulse Imager, and 4300 DNA Analyzer platforms.
IRDye 750 and IRDye 650 are not for use on LI-COR imaging systems but can be used with other imaging systems which have the appropriate detection capabilities.
IRDye near-infrared dyes are optimized for excitation and detection in the near-infrared spectrum, providing numerous benefits:
- Low background autofluorescence allows higher signal to noise ratios
- Wide range of dyes and functional groups available for multiple applications
- Available in custom derivations of fluorescent dyes and alternative pack sizes through Custom Labeling and Synthesis Services
- Excellent photostability
- Dye brightness
To learn more about LI-COR Western blotting reagents,
including IRDye secondary antibodies,
chemiluminescent substrates, blocking buffers and more,
With over 20 years of experience, LI-COR Biosciences is a leader in imaging technologies that expand analysis for genomic and proteomic applications. The technology behind LI-COR's innovative solutions is based on detection at near-infrared wavelengths. Researchers gain a competitive advantage with these technologies due to the low background and high sensitivity that can be achieved using the near-infrared spectral region. LI-COR's hardware designs include everything from laser-based excitation systems to proprietary CCD camera designs that have advanced noise reduction capabilities for peak sensitivity.
Infrared fluorophore design and production are also part of LI-COR's core competencies. Their patented IRDye® Infrared Dyes can be used to label nucleic acids, antibodies, proteins, peptides, viruses, cytokines, chemokines, and more. To remain ahead of advancing trends, new fluorophores and labeling methods are constantly in development.
Customers often remark that images produced from LI-COR products are more clear and informative. These results are an expression of the underlying technologies, which includes software that utilizes the latest techniques to visualize, analyze, and make data more understandable. Integrated solutions comprising hardware, software and labeling technologies have been responsible for many "firsts", including the first truly quantitative Western blots on the Odyssey Infrared Imaging System.
LI-COR's solutions are designed for high reliability and ease of use because they understand the impact that instrumentation problems and difficult lab procedures can have on your research.
The Standard for Western Blot Technology
As a researcher, your goal is to efficiently present the most accurate data possible. For more than 30 years, traditional chemiluminescent detection with fi lm has provided data that have been published by scientists worldwide.
Over the past decade, LI-COR® has revolutionized methods for protein detection that completely eliminate the need for film. Traditional chemiluminescent detection with film provides proven sensitivity, and LI-COR offers industry-leading technologies to maintain that sensitivity and improve your data quality to make it the clearest and most accurate it can be. LI-COR has used its expertise in optical design to provide methods for both chemiluminescent, as well as infrared fluorescence protein detection, without the use of film.
BENEFITS OF DIGITAL IMAGING
- Save valuable money on film
- Eliminate costs related to darkroom maintenance
- No need for multiple exposures
- A wide, linear dynamic range without saturation or "blown out" lanes
- Accurate detection of strong and weak bands in one exposure
- Sensitivity equal to or greater than that of film
- Eliminate the need for excessive washes and hazardous waste associated with film development
- Reduce the negative environmental impact related to film development
Whether you are looking to improve your Western blot data by simply moving to digital chemiluminescent detection or by transitioning to quantitative Western blot technology using infrared fl uorescent imaging, we will help you fi nd the best solution for you and your lab.
The Quantitative Western Blot Standard for Almost 12 Years Just Got Better!
The Odyssey CLx Infrared Imaging System is the next generation of the Odyssey Classic, the most trusted and established standard in Western blot technology.
The Odyssey CLx improves upon the Odyssey Classic through:
- Simplified User Interface
- Improved Dynamic Range
- Time-Saving Autoscan Feature
The Odyssey CLx is the most flexible and multifunctional platform of the Odyssey imaging systems. It has been designed to accommodate numerous applications, helping you to achieve results faster. The Odyssey CLx offers ease of use with one-button image acquisition as part of an autoscan feature that provides a wide dynamic range greater than six logs. This feature, along with a simplified user interface, allows you to reach excellent results simply and efficiently.
Two Independent Infrared Detection Channels
Two separate lasers and detectors simultaneously detect both fluorescent signals. The optical system employs diode lasers and solid-state detectors with long lifetimes and very low maintenance requirements. Infrared laser excitation outperforms systems that use white light, LED light sources, and filter wheels by delivering higher intensity excitation light to the fluorophore. A variety of fluorescent dyes and stains are compatible with the 700 nm and 800 nm excitation wavelengths of the two diode lasers in the Odyssey CLx. Spectral overlap is minimized by a 100 nm separation of the two detection channels, and optical filtering ensures that each detector measures fluorescence from only one of the infrared dyes.
Western Blots: Two-Color Infrared and In-Gel
Cell-Based Assays: In-Cell Western™ and On-Cell Western
Protein Detection: Coomassie-Stained Gels, Membrane and Slide Arrays
Small Animal Imaging: In Vivo, Whole Organ, and Tissue Section
Nucleic Acid Detection: Mobility Shift Assays, DNA Gel Staining (Syto® 60), and Arrays
Microwell Assays: ELISA/FLISA, Protein Arrays, and RNAi Analysis
The Odyssey Fc Imager is the first CCD imager that offers excellent infrared (IR) fluorescent and chemiluminescent imaging performance in one system. The Odyssey Fc optical system combines solid-state laser diodes at 685 and 785 nm with a low-noise, CCD camera for detection of IR and chemiluminescent signals.
Chemiluminescence detection is simplified with the Odyssey Fc Imaging System. Film and darkroom steps are eliminated, along with the hazardous waste and excessive water usage of film development.
FieldBrite™ XT technology acquires images over the full dynamic range every time, with minimal user adjustments. With the proprietary technologies of the Odyssey Fc Imaging System, a single instrument can perform quantitative IR fluorescent detection PLUS qualitative chemiluminescent detection. Publication-quality images are easy to produce with either detection method. (Because chemiluminescence is a time-dependent enzymatic reaction, its quantitative range is limited. IR fluorescence is superior for quantification.)
The Odyssey Fc Imaging System includes LI-COR Image Studio Software for fast, easy image acquisition and analysis. At the push of a button, targets of interest can be imaged over more than six logs of linear dynamic range in every acquisition.
OVERVIEW AND ADVANTAGES
Wide Linear Dynamic Range
Accurately detect strong and weak signals over a broad linear dynamic range.
Two-Color Detection and Quantification
Detect two targets simultaneously on the same membrane increases the accuracy of quantification and comparison.
Near-infrared fluorescence provides sensitivity equal or better than chemiluminescence giving you low background, high signal-to-noise, multiplexed detection, and the sensitivity of any fluorescent system.
User-friendly software with easy navigation makes acquisition and analysis straight-forward.
Direct Detection for Better Data and a Cleaner Planet
No film or darkroom needed. IRDye signals on membranes are stable indefinitely if stored properly.
Flexible System with Many Applications
The Odyssey Fc system has been used for many applications, including Western blot analysis, EMSA, In-Gel Westerns, Coomassie gel documentation and DNA gel documentation.
Pearl® Impulse Small Animal Imaging System for in vivo Imaging Applications
The Pearl® Impulse Small Animal Imaging System delivers in vivo molecular imaging with speed, sensitivity, and simplicity - at a sensible price.
In vivo imaging extends your in vitro work in to animals to unravel the complex biology of your system in a relevant context. Ready-to-use BrightSite™ imaging agents and helpful educational resources make it easy to get started immediately.
FieldBrite™ Xi technology is the heart of the Pearl Impulse Imaging System. Because of this unique technology, you get uniform illumination, exceptional sensitivity, low background noise and wide dynamic range. The proprietary FieldBrite Xi filtering scheme prevents leakage of excitation light, reducing noise and delivering superior imaging sensitivity.
Take the next step and discover why LI-COR near-infrared fluorescent technology is not just for Westerns anymore. Near-infrared fluorescence imaging is a versatile, affordable way to pursue your molecular questions - and has the potential for translation to the clinic.
Li-COR is honored to receive the 2013 Frost & Sullivan North American In Vivo Molecular Imaging Technology Leadership Awared.
OVERVIEW AND ADVANTAGES
Fluorescent Optical Imaging
Visualize biological processes in living animals with IRDye® optical agents
Innovative FieldBrite™ optical design detects smaller and deeper targets
Figure 5. Improved performance with FieldBrite Xi optics. A) NIR laser delivers excitation light in the narrow wavelength band desired, unlike LED or white light sources. B) Leakage of excitation light (yellow shading) increases image background. C) Proprietary FieldBrite Xi filtering technology dramatically reduces excitation light leakage, for decreased image background and earlier detection of small or deep targets.
Ultimate Ease of Use
One-button image capture, simple analysis software and ready-to-use BrightSite™optical agents
Monitor multiple biological targets simultaneously
With HEPA-filtered imaging beds for immunocompromised animals, docking stations and anesthesia systems.
Meet the latest innovation from LI-COR, the C-DiGit Chemiluminescent
Western Blot Scanner!
The C-DiGit Blot Scanner is a truly affordable digital replacement for film that combines the sensitivity of film with the convenience and flexibility of digital imaging.
Film-quality Western images are digitally captured with no changes to detection protocols or reagents.
Everything you love about film, without the hassles!
- No multiple exposures
- No saturation
- No developer
- No mess
- No separate analysis
- Lower expense
The C-DiGit Blot Scanner takes a new approach to chemiluminescent imaging.
It combines the advantages of film and CCD imagers to create a high-performance, highly affordable chemiluminescence imager.
Maximizing Light Collection with a Short Working Distance
The sensitivity of film is boosted by its short working distance, with very close proximity to the Western blot.
Like film, the C-DiGit sensor array uses a very short working distance (~1mm) to maximize light collection (Fig. 1). Light is captured very close to the surface of the blot, preventing loss of photons (Fig. 1 & 2).
Figure 1. Like film, C-DiGit imaging uses a very short working distance to maximize light collection.
The Western blot is placed face down, directly on the scan surface.
Figure 2. The C-DiGit Blot Scanner combines high-efficiency light collection with digital image output.
Film collects light efficiently, but the output is not digital. CCD systems provide digital images,
but with low-efficiency light collection and loss of photons.
Working distance greatly impacts the efficiency of light collection by the camera; as dictated by the inverse-square law, light intensity drops off rapidly as distance from the source increases, as dictated by the inverse square law.
The linear array of imaging sensors performs a fast-exposure line read to quickly capture data across the entire blot.
The short working distance and specialized optics of the C-DiGit Blot Scanner increase light collection by >100 fold, so comparable signals can be obtained with very short exposures.
Find out more about Image Studio Lite, freeware designed especially for the C-DiGit.
Image Studio Lite can also be used on the Odyssey CLx & Odyssey Fc
About LI-COR Biosciences
Providing answers. From the extremes of the rain forests in South America to the harsh conditions of the Antarctic, and to cutting edge research laboratories around the globe, scientists rely on products from LI-COR Biosciences to provide answers to their questions.
LI-COR first introduced scientific instruments for plant science research and quickly grew to provide scientists tools for such diverse disciplines as atmospheric research and the study of how proteins interact at the cellular level.
LI-COR Biosciences is a global leader in the design, manufacture, and marketing of high quality, innovative instruments, software, reagents, and integrated systems for plant biology, biotechnology, drug discovery, and environmental research.
More than 30,000 customers in more than 100 countries use LI-COR instruments. In addition to its Lincoln, Nebraska headquarters, LI-COR has offices in Germany and the United Kingdom. The company also sells products through a global network of distributors.
If you would like further information on any LI-COR Western Blot instrumentation or reagents
contact our Product Sales & Support Team by filling in the form below.