Fluidigm Mass Cytometry

HELIOS, A CyTOF SYSTEM

Discover your inner cell

Mass cytometry has catalysed the revolution of single-cell proteomics, enabling the most comprehensive understanding of cell phenotypes, signaling pathways and function. Helios™ dawns as the most advanced tool for cellular exploration with streamlined workflows and multimodal capabilities. It's system-level biology at single-cell resolution, on an accessible, expandable platform designed for breakthrough discovery.

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MORE THAN BEFORE

As our understanding of cellular heterogeneity's role in health and disease has grown, so has the need to precisely define an individual cell's phenotype, functional capabilities, signaling state and general health. Mass cytometry, or cytometry by time-of-flight (CyTOF®), enables greater comprehension to your research by letting you simultaneously measure more than 40 parameters for millions of cells.

PROVEN RESEARCH

Our customers' published research demonstrates transformative discovery in the life sciences.

Research areas:

Cancer research

Stem cell biology

Immunology

Vaccine research

Advancing knowledge in:

  • Basic science

  • Drug discovery

  • Translational research

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CHANNELS:

Helios expands the mass cytometry range to 135 channels ensuring comprehensive studies today, and flexibility to scale in the future as new
metal tags are developed.

PANELS:

Fluidigm offers metal-tagged antibodies as individual reagents and in preassembled kits targeting specific applications, which can be combined to build high-dimensional proteomic panels for profiling cellular systems.

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RESOLUTION:

Helios uses time-of-flight (TOF) technology to focus isotope tags into discrete, finely resolved bands.

THROUGHPUT:

Helios is barcoding enabled. This increases throughput while improving data quality, allowing you to analyse more experimental variables simultaneously.

BARCODING:

With the launch of Fluidigm's Cell-ID™ 20-Plex Pd Barcoding Kit (No. 201060), scientists can now vastly expand their throughput and get more robust data to reveal subtle phenotypes. The Cell-ID Kit enables barcoding of up to 20 samples, which are then stained and acquired as a single multiplexed sample (Figure 1). Perform your experiments with all the samples, time points and conditions you need to enable comprehensive cellular profiling.

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Maxpar Reagent Catalogue

The catalogue contains over 400 metal-conjugated antibodies to human and mouse targets covering a wide range of applications including phenotyping, cytokine expression, signaling responses, apoptosis and cell cycle, plus a variety of pre-validated panel kits. In addition, antibody labeling kits allow you to tag most IgG with your choice of 35 metals. Custom conjugation services are also available.

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ANALYSE: Cytobank

High-content Helios data becomes high-impact knowledge with the cloud-based Cytobank analysis platform. Cytobank provides an array of analysis tools, including dot plots, clustering and dimensionality reduction algorithms (SPADE and viSNE), and summary statistics tools like heat maps and dose-response curves. All are customised for efficiently extracting discoveries from mass cytometry data.

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