ProPure™ Endotoxin-Free Recombinant Proteins 

Endotoxins – lipopolysaccharides derived from Gram-negative bacteria – can trigger immune responses that compromise experimental accuracy, particularly in cell-based and in vivo studies. Sino Biological’s ProPure™ Endotoxin-Free Recombinant Proteins are produced under animal-free conditions using a proprietary purification workflow that delivers exceptionally low endotoxin levels (<0.1 EU/μg). Designed to meet the stringent demands of immunology, inflammation, and preclinical research, ProPure proteins provide confidence in experimental results by reducing background immune activation and ensuring reproducible data. 

Endotoxin contamination is a hidden but significant variable in biological research. Even trace amounts can lead to false-positive immune responses in assays or alter cytokine production in cell models. Traditional recombinant protein expression systems, particularly those using E. coli, often yield proteins contaminated with endotoxin residues, making additional purification essential before sensitive applications.

Sino Biological’s ProPure™ line was developed to address this critical challenge. Through an advanced combination of expression system optimisation, multi-step purification, and endotoxin removal, ProPure proteins achieve endotoxin levels below 0.1 EU/μg—surpassing industry standards. This makes them particularly well-suited for applications such as:

• In vivo animal studies requiring endotoxin-free reagents
• Cell stimulation and activation assays where innate immune pathways are measured
• CAR-T, vaccine, and antibody development research
• Inflammation and immunology studies sensitive to TLR activation

All ProPure proteins are animal-free, removing variability associated with serum-derived materials. The streamlined production process also retains native protein conformation and biological activity – critical for achieving consistent results across experiments.

Compared to standard recombinant proteins, ProPure reagents minimise background cytokine induction and non-specific immune activation, helping researchers distinguish true biological responses from endotoxin artefacts. By ensuring reproducibility and reliability, these proteins support high-quality data generation in translational and preclinical research environments.

With growing emphasis on data integrity and regulatory compliance, ProPure™ Endotoxin-Free Proteins represent a valuable upgrade for any lab working with immune cells, organoids, or animal models. Their consistent low-endotoxin profile helps meet the rigorous standards required for translational and preclinical workflows. 

When precision matters, ProPure™ Endotoxin-Free Recombinant Proteins provide unmatched purity and performance. By eliminating one of the most common sources of experimental variability, researchers can trust that their immune-related findings reflect biology – not contamination.