Common Challenges in RNA Extraction and How to Solve Them

RNA extraction is a crucial step in molecular biology, but it often comes with obstacles that can impact downstream applications such as RT-qPCR, sequencing, and microRNA analysis. Researchers in Australia and New Zealand frequently face challenges with low yield, degraded RNA, or inhibitor contamination. Fortunately, Norgen Biotek’s Total RNA Purification Kits, distributed by Millennium Science, offer reliable and comprehensive solutions.

Working with small or precious samples often results in low RNA recovery.

Solution: Norgen’s Total RNA Purification Kits are optimised to maximise yield across a wide range of sample types, including blood, plasma, tissues, and cultured cells. With just one kit, researchers can extract total RNA covering all sizes from large mRNA down to small RNA species such as microRNA ensuring nothing important is lost.

RNA is notoriously unstable and highly susceptible to degradation during purification.

Solution: Norgen’s patented silicon carbide (SiC) resin technology protects RNA integrity throughout the process. This unique chemistry binds and isolates all RNA species including fragile small RNAs without the need for harsh chemicals like phenol, chloroform, or alcohol precipitation. By operating under gentler conditions than traditional silica methods, the SiC resin preserves RNA integrity and delivers intact RNA ready for sensitive applications such as sequencing, RT-qPCR, and microRNA profiling.

Contaminants such as heme, salts, or proteins can inhibit enzymatic reactions and compromise results.

Solution: Norgen kits efficiently remove inhibitors during the purification process, providing RNA that is pure, stable, and immediately suitable for downstream workflows, from gene expression analysis to diagnostic assay development.

Take the uncertainty out of RNA extraction. Request a quote or contact Millennium Science today to learn more about Norgen Biotek’s complete range of RNA purification solutions.

As the exclusive distributor of Norgen Biotek products in Australia and New Zealand, Millennium Science, based in Melbourne, provides researchers with responsive technical support, and competitive pricing. By combining innovative technology with local expertise, Millennium Science ensures scientists have the tools they need to achieve reproducible, high-quality results.