Biotium

Best Seller!

• Safer alternatives to Ethidium Bromide (EtBr), SYBR® Safe, and others
• Ultra-sensitive nucleic acids gel stains: much more sensitive than EtBr and SYBR Safe

Efficient, easy-to-use kits for labeling antibodies and other proteins with Biotium’s superior CF® Dyes and other labels.

• FAST & SIMPLE ANTIBODY LABELING
  Easy-to-use with minimal hands-on time, Mix-n-Stain™ kits require no purification after labeling
• OPTIMAL LABELING OF NANOBODIES
  Mix-n-Stain™ kits specific for labeling nanobodies (single variable or VHH domains)
• PE, APC, PERCP, & TANDEM LABELING
  Antibody labeling for flow cytometry made easy
• MIX-N-STAIN™ HRP, AP, & GOX KITS
  Make your own enzyme-coupled antibodies
• SMALL LIGAND LABELING KITS
  Create fluorescent SNAP-tag®, CLIP-tag™, Halotag® & other ligands, no purification required
• CF® SE DYE PROTEIN LABELING KITS
  Larger scale kits, for optimized protein labeling & purification
• VIVOBRITE™ NEAR-IR LABELING KITS
  Kits for labeling antibodies with our superior near-IR CF® Dyes, for in vivo small animal imaging

Biotium offers a wide-selection of assay kits for cell viability and cell death for microplate reader, flow cytometry, or fluorescence microscopy

• Cell quantitation for microplate reader
  Absorbance-based cell quantitation assays
  Fluorescence-based quantitation assays
• Luminescence-based cell quantitation
  ATP-Glo™ Bioluminometric Cell Viability Assay
• Cell division tracking dyes for flow cytometry or cell tracing
  ViaFluor® SE Cell Proliferation Kits
• Mitochondrial membrane potential dyes
  MitoView™ Dyes
  JC-1 and Other Classic Mitochondrial Membrane Potential Dyes
• Fluorescent live & dead cell stains
  Viability/Cytotoxicity Assay Kit
  Dead Cell Nucleic Acid Stains
• Fixable dead cell stains
  Live-or-Dye™ Fixable Dead Cell Stains
  Sampler Kits Available
  
• Caspase assays & apoptosis inducers
  NucView® Caspase Substrates for Live Cells
  Caspase-3 DEVD-R110 Fluorometric & Colorimetric Assay Kit
  Caspase-3 DEVD-R110 Fluorometric HTS Assay
  Ac-DEVD-AMC
  Ac-DEVD-R110
  Ac-DEVD-CHO Caspase-3 Inhibitor
  Staurosporine
  Ionomycin, Calcium Salt
• Apoptotic/Necrotic staining kits & Annexin V
  Dual Apoptosis & Necrosis Assay Kits
• Tunel kits & stains for fixed cells & tissues
  TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3′ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA in fixed cells or tissue sections. Biotium offer TUNEL kits with dUTP conjugates of our bright and photostable CF® dyes with green, red, or far-red fluorescence.

Non-specific fluorescence background can have several causes, including sample autofluorescence, non-specific antibody binding, and electrostatic interactions of fluorescent dyes with proteins and blotting membranes. TrueBlack® reagents for IF staining and western blotting block background from multiple sources for optimal specificity.

• TRUEBLACK® LIPOFUSCIN AUTOFLUORESCENCE QUENCHERS
  Clears the way for immunofluorescence of human tissue.
• NEW: EVERBRITE TRUEBLACK® HARDSET MOUNTING MEDIUM
  The only mounting medium that quenches lipofuscin background and preserves fluorescent signal.
• TRUEBLACK® IF BACKGROUND SUPPRESSOR SYSTEM
  Blocks IF background from multiple sources.
• TRUEBLACK® WESTERN BLOT BLOCKING BUFFER KIT
  Superior blocking for fluorescent/near-IR westerns.

Biotium offer a selection of membrane-permeant and impermeant fluorescent indicator dyes for calcium and other ions, as well as calibration buffers, ionophores, and accessory reagents.

• Calcium indicators
• Calcium chelators
• Calcium accessory reagents
• Chloride & Zinc indicators
• pH-responsive dyes

Biotium offers a variety of stains for different organelles and cellular structures.

• Nucleus
• Lysosomes
• Cytoskeleton
• Cytoplasm
• Mitochondria
• Lipid droplets
• Exosomes & Extracellular vesicles (EVs)
• Membrane & Cell Surface
• Vesicle trafficking
• Neuronal stains

Learn more

Explore Cellular Stains Comparison Guides to browse targets, applications, fixability, and stains for different organisms.

Find a membrane stain that fits your workflow with Biotium’s Membrane and Cell Surface Stain Comparison Guide.

• GELRED®
  The most sensitive red fluorescent DNA gel stain, proven safer than EtBr
• GELGREEN®
  A safer and more sensitive alternative to SYBR® Green I green fluorescent gel stains
• PAGE GELRED®
  Similar to our classic GelRed® stain, but made to stain DNA in PAGE gels with high sensitivity
• DNAZURE® VISIBLE GEL STAIN
  A novel visible blue DNA gel stain, extremely sensitive and great for cutting out bands for cloning
• CLASSIC GEL STAINS
  Featuring high-quality and low cost forms of Oxazole Gold (SYBR® Gold) and Thiazole Green (SYBR® Green I)

Biotium offers substrates for many common enzymes, including luminescent substrates for Firefly and Renilla Luciferases, luciferin and coelenterazine;  fluorometric NucView®  caspase-3 substrates; various protease substrates;  and other enzyme substrates.

• EXCEPTIONALLY BRIGHT & SPECTRALLY UNIQUE CF® DYES
  Superior brightness & signal-to-noise over alternative fluorophores
• PRIMARY ANTIBODIES AND ANTIBODY LABELING KITS
  Rapid & convenient antibody labelling kits & over 1000 monoclonal antibodies
• LIVE-OR-DYE™ FIXABLE VIABILITY STAINING KITS
  Bright, fixable dead cell stains, available in 14 colours with options for spectral flow
• DEAD CELL SELECTIVE COUNTERSTAINS
  High purity & low cost forms of TOTO®-1, YOYO®-1, TOTO®-3, & other cell-impermeant DNA stains
• VIAFLUOR® CELL PROLIFERATION
  Non-toxic, fixable proliferation dyes designed as improvements to CFSE
• CELL CYCLE ANALYSIS
  Novel & classic stains for analysing DNA content/cell cycle by flow cytometry
• NUCVIEW® CASPASE-3 SUBSTRATES
  Monitor apoptosis with the worlds only live-cell, real-time caspase-3 substrates
• CELL VIABILITY ASSAYS
  Dyes for monitoring mitochondrial membrane potential & apoptosis/necrosis status
• BUFFERS, FIXATIVES, OTHER ACCESSORIES
  Featuring optimized fixation & permeabilisation kits & buffers

Biotium offers bioconjugates for a variety of applications, available with our bright & photostable CF® dyes and other labels.

• ANNEXIN V
  Apoptotic cell marker
• ALPHA-BUNGAROTOXIN
  AchR probe labels neuromuscular junctions
• CHOLERA TOXIN & TRANSFERRIN
  Plasma membrane probes & endocytic tracers
• CON A, WGA, AND PNA LECTINS
  Glycoprotein probes, cell surface stains
• DEXTRANS & BSA
  Fluid phase tracers
• NUCLEOTIDE CONJUGATES
  For probe synthesis, TUNEL assay, or receptor probes
• PHALLOIDIN
  F-Actin stain for cytoskeleton
• STREPTAVIDIN & BIOTIN
  Biotinylated probes, labeled streptavidin & biotin
• PRIMARY & SECONDARY ANTIBODIES
  Conjugated with our superior CF® dyes and other labels

• CF® DYE ANTIBODIES AND CONJUGATES
  Includes antibodies, bioconjugates, labelling kits, and CF® Dyes for super-resolution applications
• TYRAMIDE SIGNAL AMPLIFICATION
  21 bright and photostable fluorescent CF® Dye tyramides. Biotin & DNP conjugates also available
• ANTIFIX™ ANTIGEN RETRIEVAL BUFFER
  Universal antigen retrieval buffer for unmasking a wide variety of targets
• TRUEBLACK® BACKGROUND REDUCERS
  Buffers specially formulated for blocking background from lipofuscin autofluorescence, charged dyes, and other sources
• EVERBRITE™ MOUNTING MEDIUM
  Offers advanced protection from photobleaching. Available with DAPI or far-red NucSpot® counterstains. Hardset medium with TrueBlack® also available.
• BUFFERS AND OTHER ACCESSORIES
  Featuring permeabilization and fixation buffers, coverslip sealant, and pap pens

• Near-IR CF® Dye Annexin V Conjugates
  Annexin V conjugated to our exceptional near-IR CF® dyes. Lyophilized and preservative free.
• Near-IR Cytoplasmic Membrane Stains
  CellBrite™ Near-IR dyes and DiR are lipophilic cytoplasmic membrane dyes with fluorescence in the near-IR range.
• Vivobrite™ Rapid Antibody Labeling Kits for Small Animal Imaging
  Everything you need to label and purify antibodies for near-infrared small animal imaging. Featuring our exceptional NIR CF® dyes.

• FIREFLY & RENILLA ASSAY KITS
  Super sensitive flash-type or long half-life glow assays. Available in lyophilised format.
• ECONOMICAL DUAL REPORTER ASSAY
  Measure Firefly & Renilla in the same tube. Substrates provided separately for flexibility.
• LUMINESCENT CELL VIABILITY
  ATP-Glo flash-type viability assay. Detect as little as 0.01 pmol ATP or a single viable cell.

• EVAGREEN® DYE FOR QPCR AND OTHER APPLICATIONS
  Bright, sensitive and environmentally safe; ideal for qPCR and many other applications
• EVARUBY™ DYE FOR QPCR & HRM®
  A spectrally unique red qPCR dye that expands probe-based multiplexing
• FORGET-ME-NOT™ EVAGREEN® QPCR MASTER MIX
  2-color or 1-color tracking allows you to spot pipetting errors at a glance
• FORGET-ME-NOT™ UNIVERSAL PROBE QPCR MASTER MIX
  Suitable for all fluorescent probe-based PCR methods including TaqMan® or Molecular Beacons
• N-FLUX™ 5X DIGITAL PCR MASTER MIX
  High performance master mix for chip-based digital PCR (cdPCR) platforms.
• CHEETAH™ HOT-START TAQ POLYMERASE
  Chemically modified hot-start taq; no activity at RT and activates in only 2 minutes
• VERIFLUOR™ FAR-RED PASSIVE DYE
  Offers reliable and steady PCR normalization. Functions as ROX alternative and direct replacement for Mustang Purple™.
• EVAEZ™ FLUOROMETRIC POLYMERASE ACTIVITY ASSAY
  Easy, accurate, and non-radioactive nucleic acid polymerase activity assay
• PCR ACCESSORIES & RELATED PRODUCTS
  dNTPs, ROX reference dye, hot-start/warm-start enzyme modification kits, and more

Biotium have a growing collection of more than 2000 monoclonal antibodies validated in IHC and other applications, many verified for specificity in HuProt™ Protein Array. Available with bright and photostable CF® dyes, R-PE, APC, PerCP, HRP, AP, biotin, or purified. Affordable 100 uL trial sizes. Refine your selections using the criteria in the left sidebar, or search using our Antibody Finder.

• Cancer research
• Organelles
  Monoclonal antibodies against a variety of organelle markers, available conjugated to bright & photostable CF® dyes and other labels.
• Exosomes/EVs
  Validated antibodies against exosome targets, conjugated to bright & photostable CF® dyes and other labels.
• Microbiology
  Primary antibodies against microbiological targets, including bacterial, viral and fungal proteins. Available in a wide variety of dye colors and tags.
• Neuroscience
  Monoclonal antibodies available conjugated to our bright & photostable CF® dyes and other labels.
• Anti-Human Immunoglobulin
  Fluorescent secondary antibodies with specificity against human IgG and human IgA.
• Isotype Controls

Biotium offers our bright & photostable CF® Dyes, other dyes, and biotin with a full selection of reactive chemistries.

• SUCCINIMIDYL ESTERS
  Amine-reactive NHS esters & labeling kits
• MALEIMIDE & MTS
  Thiol-reactive dyes & thiol modifying reagents
• AMINOOXY & HYDRAZIDE
  For labelling carbonyl groups or carbohydrates
• DYES WITH AN AMINE GROUP
  Aldehyde-reactive dyes
• BIOORTHOGONAL LABELING
  Azides, alkynes, BCN, tetrazine, & TCO
• TYRAMIDES
  Tyramide dyes & TSA Signal Amplification Kits
• FREE DYES
  Dye free acids & polar tracers
• BIOTINYLATION REAGENTS
  Reactive biotins and other biotin compounds

Dyes and probes for detecting Nitric Oxide (NO) or reactive oxygen in cells.

• Nitric Oxide Synthase Inhibitors
• Nitric Oxide (NO) Detection
• Nitric Oxide (NO) Donors
• Reactive Oxygen Species Indicators

• SECONDARY ANTIBODIES, NOT CROSS-ADSORBED
  Suitable for single target staining, may cross-react with IgG from other species
• SECONDARY ANTIBODIES, HIGHLY CROSS-ADSORBED
  Minimal cross-reactivity with other target species, for multiplex staining
• ISOTYPE-SPECIFIC ANTI-MOUSE SECONDARY ANTIBODIES
  Useful for multiplexing with monoclonal antibodies of different isotypes
• STREPTAVIDIN, ANTI-BIOTIN & EPITOPE TAG ANTIBODIES
  Detect biotin, GFP, RFP, FITC, & a variety of epitope tags
• SINGLE LABEL SECONDARY ANTIBODIES FOR STORM
  Antibodies labelled with (d)-STORM-compatible CF® dyes with average degree of labelling (DOL) of one
• ANTI-HUMAN IGG
• ANTI-HUMAN IGA & IGM
• ANTI-HUMAN KAPPA LIGHT CHAIN
• ANTI-HUMAN LAMBDA LIGHT CHAIN

Viability PCR is a powerful technology for the sensitive and rapid detection of viable microorganisms. Unlike time-consuming culturing methods, qPCR is a fast and sensitive method of detection. However, normal qPCR does not distinguish between live and dead cells. With v-PCR using PMAxx™ or PMA, you get the speed, sensitivity and specificity of PCR, plus quantifiable viability. And because no culturing is required, you can even detect viable but not culturable (VBNC) bacteria. The v-PCR technology can be applied not only to bacteria but to other organisms like yeast, viruses, eukaryotes, and archaea.

• VIABILITY PCR DYES
  Dead cell-specific dyes covalently modify DNA, allowing selective PCR amplification from live cells
• PMA & PMAXX™ V-PCR DYES
  PMAxx™ is our newest v-PCR dye, with improved live/dead discrimination compared to our original PMA
• VIABILITY PCR STARTER KITS
  Easily get started with viability PCR, with any cell type
• PMA ENHANCER
  Improve PMA & PMAxx™ modification of dead gram-negative bacteria
• PHOTOACTIVATION DEVICE
  PMA-Lite™ LED photolysis device for sample tubes
• STRAIN-SPECIFIC V-PCR KITS
  Everything you need for v-PCR of common strains

• PROTEIN GEL STAINS
  Fast, easy & non-toxic One-Step Stains
• VERSABLOT™ PROTEIN PRESTAIN
  Super-sensitive and reversible protein band quantitation in gels or blots
• PROTEIN THERMAL STABILITY ASSAY
  GloMelt™ is highly sensitive & tolerant of detergent
• PROTEIN QUANTITATION
  AccuOrange™ highly sensitive fluorescence protein assay
• REAGENTS FOR WESTERN BLOTTING
  Conjugates and accessories for western blotting