PacBio

HiFi reads let you accurately detect all types of variants, from single nucleotide to structural variants, with high precision and recall and phase haplotypes, even in hard-to-sequence regions of the genome. PacBio HiFi reads is the only long read sequencing technology that provide accuracy of 99.9%, on par with short reads and Sanger sequencing. With HiFi reads, you no longer have to compromise long read lengths for high accuracy sequencing to address your toughest biological questions.

Game-changing sequencing by binding (SBB) chemistry on the Onso system surpasses traditional sequencing by synthesis (SBS) technology. With near-perfect accuracy boasting 90% of bases at ≥Q40+, 15× higher accuracy, low duplication, negligible index hopping, and the ability to conquer challenging regions, SBB is redefining the landscape of short-read sequencing for research and translational applications.

Kinnex is transforming the landscape of transcriptome research with isoform discovery and microbiome community profiling. Kinnex kits are based on the MAS-Seq method, which concatenates smaller amplicons into larger fragment libraries for throughput increase. Whether you are doing full-length RNA sequencing, single-cell RNA sequencing or 16S rRNA, Kinnex offers full-length isoform discovery and abundance information, cell type-specific isoform discovery, and species-level metagenomics community identification that are difficult to capture with short reads.

PureTarget uses the CRISPR-Cas9 system to generate targeted native DNA libraries. This amplification-free approach retains epigenetic signals and has no PCR artefacts or errors. With a streamlined workflow, multi-gene panels, and sample multiplexing, now you can screen the most challenging genomic regions at scale.

The PureTarget product line includes a panel of 20 repeat expansion loci relevant to neurodegenerative disease. Learn more about PureTarget-enabled comprehensive characterisation of dark genes like FMR1, C9orf72 and RFC1.

Innovative solutions for high molecular weight (HMW) DNA sample prep. 

Nanobind extraction kits are based on proprietary Nanobind disks that can be used for the extraction of DNA from diverse sample types, including blood, cell, and tissue samples. With this technology, researchers can extract longer fragments from high-quality DNA in less than two hours, with minimal sample input and without the use of harmful chemicals or mechanical homogenisation.

How does Nanobind work?

Nanobind kits contain specific reagents to process and lysate diverse sample types. Following lysis, the DNA binds to Nanobind disks which shield bound DNA from damage during extraction, resulting in HMW DNA. A final Short Read Eliminator (SRE) size selection step helps remove DNA fragments below 10 kb.

PacBio offers a portfolio of long- and short-read sequencing systems that help you make discoveries that can change the world. Whether it’s interrogating difficult genomic regions, detecting novel transcript isoforms, or uncovering rare variants in liquid biopsies, our systems let you see complete data.

Revio System | Long-read sequencing

The Revio system with SPRQ chemistry delivers 120 Gb per SMRT Cell, ideal for large-scale projects. Its fully automated workflow runs up to 4 SMRT Cells simultaneously, maximising efficiency and throughput.

Vega Benchtop System | Long-read sequencing

The Vega benchtop system delivers 60 Gb per SMRT Cell —ideal for labs that value speed and simplicity. Its user-friendly interface and single SMRT Cell configuration put you in full control for streamlined, immediate sequencing.

Onso System | Short-read sequencing

Powered by sequencing by binding (SBB) chemistry, the Onso system delivers ground-breaking short-read performance with 90% Q40 accuracy. This 15x accuracy boost increases sensitivity for rare variant detection, reduces sequencing needs, and lowers cost per sample.

PacBio HiFi sequencing delivers highly accurate, single-molecule long reads for analysing both DNA and RNA, allowing you to explore the full extent of human genetic variation with a more comprehensive view of the genome, transcriptome, and epigenome than ever before. The benefits of HiFi sequencing — and its numerous applications in human genomics — are ready to enable your next great discovery.

• Genetic testing – Illuminate complex regions of the genome that are crucial for disease identification.
• Clinical research – Get detailed information into complex genetic regions by detecting structural variants and repeat expansions to accelerate personalized medicine in clinical research.
• Population genetics – Utilise scalable long-read sequencing to explore genomic variation, driving precision medicine initiatives in diverse populations.
• Human research – Learn how highly accurate sequencing can help resolve complexities in human disease research.
• Biopharma – Explore how highly accurate sequencing can be used to support research and development of potential treatments for disease.

Characterise, assemble, and gain a better understanding of microbial communities and metagenomics.

• Public health and surveillance – Protect public health with comprehensive genomic surveillance solutions that deliver the complete information you need for accurate characterisation and tracking of all pathogens circulating in the community.
• Infectious disease and microbiology research – Advance human health with new insights into the viruses, bacteria, and protozoa that cause infectious disease. Reveal the biology of host-pathogen dynamics, drug resistance, and immune escape.
• Discovery and industrial microbiology – Address the global need for novel therapeutics, environmental remediation, and efficient chemical production by mining even the most difficult to sequence microbial genomes and complex microbial communities.
• Plant and animal microbiology – Explore the biology of microbial symbionts, pathogens, microbiomes and phytobiomes and the roles they play in crop yields, livestock health, and the preservation of wild animals and natural habitats.

In the face of a changing climate and growing populations, highly accurate long-read sequencing technology gives plant and animal scientists access to actionable genomic insights that other technologies cannot provide.

• Agrigenomics – Accelerate breeding programs and increase resilience to pests, disease, and climate challenges.
• Biodiversity – Explore the biodiversity of populations, species and ecosystems to build a more detailed view of the biome.
• Plant and animal biology – Address questions about evolution, development, gene expression, transcription regulation, and more.

In order to fulfill the promise of cancer precision medicine, a better understanding of cancer’s complex biology is needed. PacBio highly accurate sequencing allows you to uncover novel isoforms, fusions, and structural variants with exceptional accuracy.

• Long-read RNA sequencing for isoform and fusion discovery – Cancer exhibits widespread RNA dysregulation. The Iso-Seq method uncovers the full repertoire of alternative splicing and fusion events.
• Long-read whole genome sequencing (WGS) for somatic variant detection – Structural variants (genomic differences ≥50 base pairs) are one of the main drivers of cancer. Most structural variants are too large to reliably discover with current short-read DNA sequencing technology.
• Highly accurate short-read sequencing for rare variant detection – Using the newly developed short-read sequencing platform you can achieve unprecedented sensitivity for detecting rare variants

Whether you are conducting research on novel potential drug targets, biomarkers, or therapeutics such as gene therapies, PacBio offers a sequencing solution that can improve your chances of success and may reduce your time to market. Sequencing has become an integral part of the research and development process and helps to develop the critical understanding of determinants of health and disease on the molecular level.

HiFi sequencing allows you to overcome many limitations that short-read or less accurate long-read methods may pose and can play important roles in enhancing your research on

• Target or biomarker identification
• AAV gene therapies
• CRISPR-Cas9 gene editing
• Biologics development