Introducing 10x Genomics‘ Visium HD 3’, a novel assay for unbiased cross-species spatial gene expression profiling of fresh frozen tissue sections mounted on a standard glass slide. The Visium HD’s array has a gapless design that enables integration of unsupervised gene expression clustering data with microscope H&E images from the same tissue section, allowing precise profiling of finer anatomical features with high tissue coverage at single cell-scale resolution.

Furthermore, this novel reverse transcription-based assay generates cDNA products compatible with both short reads and long reads sequencing, extending its applications beyond gene expression analysis to enable immune profiling and the discovery of isoforms and novel transcripts on a spatial level.

Improve cell-type resolution with nuclei expansion–based cell segmentation available in Space Ranger version 4.0 software. Importantly, Space Ranger v4.0 now supports cell and nucleus segmentation with H&E images. So you can reprocess existing Visium HD data through the new Space Ranger pipeline to derive these nuclei and cell segmentation outputs alongside the square bins

1. Input a brightfield image of your H&E-stained tissue into the Space Ranger pipeline
2. The software performs nuclei detection and expansion
3. The Space Ranger pipeline outputs custom binning files viewable in Loupe Browser software

The new version can be found at: https://www.10xgenomics.com/support/software/space-ranger/latest

Some other notable features are:

• A new UMI-based registration algorithm for Visium HD and Visium HD 3′ data.
• You can now visualize and analyse Visium HD / Visium HD 3′ cell segmentation in Loupe Browser v9.

Learn more about the Visium HD 3’ assay in this introductory webinar, including a Q&A with product experts.

Secure funding with confidence using our comprehensive 10x Genomics grant application resources, designed to help you articulate the scientific value, feasibility, and innovation of your single‑cell and spatial biology projects. Millennium Science partners with 10x Genomics to bring you expertly curated resources that strengthen your research proposals and increase your chances of success.

10x Genomics technologies are widely used in peer‑reviewed research, enabling high‑resolution single‑cell and spatial profiling across diverse biological systems. With over a decade of innovation, extensive validation, and thousands of publications demonstrating impact, these platforms help you justify the scientific significance and methodological rigour of your proposals.

Enhance your applications with ready‑to‑use materials for key 10x Genomics platforms:

Our downloadable guides provide:

• Clear technology descriptions that strengthen methodological justification
• Workflow and sample preparation overviews aligned with best practice
• Performance metrics and validation data to support feasibility claims
• Data processing and analysis recommendations
• Sample and project size considerations for budgeting
• Up‑to‑date references and supporting publications

Need help framing your aims or justifying your technology choice for upcoming grant application? Book a project discussion with one of our specialists. Our team can help with experimental design, sample requirements, technology selection and data analysis planning, providing valuable input for competitive funding applications.