Most popular Abcam secondary antibodies by species:
Goat Anti-Rabbit IgG: AF®488 | AF®647 | AF®594 |HRP
Goat Anti-Mouse IgG: AF®488 | AF®647 |HRP
Donkey Anti-Rabbit IgG: AF®488 | AF®647
Donkey Anti-Mouse IgG: AF®488 | AF®647
Cross-adsorbed: Goat Anti-Mouse AF®488 | Goat Anti-Rabbit AF®647 | Donkey Anti-Rat AF®647
Save time with consistent, sensitive and specific immunoassay tools across 1,000 targets.
Matched antibody pairs are a capture antibody and a detection antibody, used in sandwich ELISAs to bind to the protein of interest. Just like any other antibody, these pairs need to be specific and sensitive to give the best results. Abcam uses recombinant antibodies in their matched antibody pairs, for unrivaled reproducibility and outstanding sensitivity. Develop your own immunoassay with pairs that can be broken down to individual capture and detector antibodies, paired with other proteins or components, and are available in carrier-free formats.
ELISA kits are kits containing all the reagents and tools you need to conduct an ELISA. High-quality matched antibody pairs as the base of Abcam's ELISA kits give you reproducible results over time. SimpleStep ELISA® kits are kits with recombinant matched antibody pairs, designed to reduce your experiment time.
Benefits of Abcam's SimpleStep ELISA® kits:
They also provide guides, webinars, and other resources to help you get the best results in your immunoassay experiment. Whether you're bringing your expertise up to date or getting started for the first time, learn everything from the basic principles of ELISA to how to analyse your data.
Quantify your protein of interest with one of Abcam's specific and sensitive ELISA kits covering over 1,000 targets.
Easily adapt or scale up your immunoassays with matched antibody pairs in carrier-free format (BSA, glycerol, and azide free).
Combine assays for many analytes in a single reaction volume with Abcam's customizable FirePlex multiplex assays.
Highly sensitive results in 90 minutes or less with just one wash step.
Clear and reproducible staining images require high quality reagents and accessories. Abcam offer over 800 kits, reagents and accessories for tissue and cell imaging, designed to help you obtain high quality staining results in your immunohistochemistry (IHC) and fixed and live cell imaging experiments.
The nuclear, cytoplasmic, membrane, and organelle antibodies you need.
Detecting and quantifying cellular processes such as cell death or metabolic reactions requires high quality tools. Abcam have over 750 cellular and biochemical assays, covering a wide range of research areas including Apoptosis, Cell signaling and Metabolism, to help you quickly obtain accurate and reproducible results.
Quantify reactive oxygen species (ROS) in one simple step with DCFDA.
Abcam have an extensive range of proteins and peptides including cytokines, growth factors, signaling proteins, viral and bacterial antigens, human immunoglobulins and more. Guaranteed high quality and purity, they are validated in popular applications and verified by you through reviews and high impact factor publications.
View Abcam's latest highest grade proteins specifically designed to provide consistency within your cell culture experiments.
Reduce experimental variables associated with animal or mammalian pathogens by using proteins produced in animal free conditions.
View Abcam's popular Immunoglobulin proteins including native and recombinant in multiple species of origin.
Browse Abcam's extensive range of high quality guaranteed cytokines, chemokines and growth factors.
View Abcam's range of fully validated biologically active proteins.
Explore the cytokine network and find key products to support your research with Abcam's interactive poster.
Abcam's protein research tools are designed to give you consistent and accurate results. Their portfolio includes antibody arrays and kits and reagents for western blot, protein electrophoresis , cellular fractionation and protein purification and quantification.
High performance resins for protein/antibody purification and immunoprecipitation.
For easy isolation of mitochondria from cell cultures and tissues.
Biochemical molecules can affect the specific function of a protein, leading to a change in the cell process. Gain access to a growing product line that includes a vast selection of innovative products and services to support drug discovery, life science, medical, and biomedical research in high purity and convenient sizes.
You will find high-quality, highly cited ligands, modulators of ligand and voltage-gated ion channels, cell signaling pathway tools, and enzyme inhibitors to support your research within the range. Small‐molecule enzyme inhibitors account for approximately 50 % of approved drugs and can also be powerful chemical tools for probing biological systems. Most of these inhibitors displace either substrate or cofactor, providing a clear target for ligand discovery.
Abcam's small molecules, agonists, antagonists, activators, and inhibitors are trusted products backed by superior customer service and the continuous Abcam commitment to improved quality. To help you attain better, faster results, Abcam strive to achieve the highest purity and minimize the presence of solvent to exceed the current market quality levels.
In addition, Abcam also offer multiple chemicals for permanent and temporary labeling of organelles, proteins, and other molecules, as well as gold standard labeling dyes, such as DAPI, Hoechst, and JC-1. Their dyes and stains include reagents for the covalent linkage of target proteins, fluorescent probes, reactive oxygen and nitrogen species (ROS/RNS) detection tools, and click-chemistry reagents.
Save time with water soluble, simple stock solutions. Simply add 1 mL of water and start your experiment.
Discover Abcam's exclusive Pitstop® compounds to selectively inhibit clathrin-mediated endocytosis.
Abcam's portfolio of cell lines and lysates includes CRISPR knockouts, iPSC-derived neurons, along with standard cell lysates from a range of species and immortalized cell lines, and both healthy and diseased tissue lysates. All their cell lines and lysates are validated in key applications and verified through abreviews by you.
Engineered and ready for you, find reliable, off-the-shelf, single-gene knock-outs in a number of immortalized cell backgrounds.
Discover Abcam's range of knock-out cell lysates and get quick and simple solutions for your validation studies.
Your edit, our expertise - engineered and ready for you.
Multiplex miRNA assays with FirePlex particle technology enables simultaneous profiling of 65 miRNAs directly from small amounts of biofluid or FFPE, without RNA purification or pre-amplification, with assay readout on a standard flow cytometer .
To learn more about the performance of FirePlex miRNA assays and their comparison with the other platforms, check out the tech notes below:
Benchmarking the FirePlex® miRNA Assay
MicroRNA profiling using the Multiplex Circulating miRNA assay
Design a custom particle panel to profile miRNAs of choice for your experiment
Download software and guides for the FirePlex Analysis Workbench.
Multiplex assays combine several target analytes in a single reaction volume to enable researchers to measure multiple biomarkers simultaneously. By measuring multiple analytes in the same reaction volume, multiplex assays allow you to harvest more insightful data to inform better decision-making, accelerate research and increase the pace of discovery.
Abcam offer a wide range of multiplex assays, such as antibody arrays and FirePlex ® assays, including their class-leading high-throughput FirePlex-384 and FirePlex-1536 formats.
Abcam's FirePlex multiplex assays are designed with multiplexing in mind, to help you overcome common challenges in biomarker analysis and accelerate discovery. FirePlex particle technology allows us to build multiplex assays with high plex, sensitivity, and high throughput capacity (in 384-well and 1536-well plate formats).
Compared to traditional multiplex assays, FirePlex assays provide a faster, automatable workflow, smaller sample requirements, and easy readout on both flow cytometers and high-content imagers, saving hands-on time and sample. With the high sensitivity and unrivaled reproducibility capabilities of recombinant antibodies, FirePlex multiplex assays power your progress with robust, consistent data.
Abcam offer FirePlex assays for multiplex proteins (FirePlex immunoassays) or miRNA (FirePlex miRNA assays) analysis, including:
Abcam's antibody arrays, which are similar to ELISA but with enhanced multiplexing capabilities, can analyze hundreds of different proteins simultaneously at significantly lower cost, time, and effort.
Quantitative and semi-quantitative arrays offer 40 and 96 analyte multiplexing respectively, for comparative analysis of cytokines, chemokines, growth factors, as well as many other biological proteins and pathways in human, mouse, rat, and other species.
Accelerate your path to clinical success with Abcam's high-throughput, automation-friendly format, to facilitate high-volume biomarker testing throughout drug discovery:
About Abcam identifies, develops, and provides high-quality biological reagents and tools which are essential in a wide range of fields and applications including drug discovery, diagnostics and basic research.
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