Maximum # of fragments assembled: 5
Cloning efficiency: 90% full-length clones
Insert size range: 500-32,000 bp
Total construct size range: 100,000 bp
Reaction time: 60 minutes
Codex DNA's Gibson Assembly® Ultra reagent kit provides the longest, high-fidelity builds available.
Maximum # of fragments assembled: 15
Cloning efficiency: 95% full-length clones
Insert size range: 100 - 100,000 bp
Total construct size range: 1,000,000 bp
Reaction time: 80 minutes
Gibson Assembly® RapidAMP™ takes it one step further and provides amplified, transfection-ready quantities of >10 μg of DNA in an endotoxin-free reaction.
Maximum # of fragments assembled: 4
Cloning efficiency: 95% full-length clones
Insert size range: 300 - 1,800 bp
Total construct size range: 10,000 bp
Reaction time: 80 minutes + 4 hours
The benchtop RapidAMP™ process begins with a linearised vector plus up to three linear DNA insert fragments, 300-1800 bp each. All are assembled via Gibson Assembly® technology to generate a circular cloning product.
The workflow produces clean, high-yield DNA material. A representative gel below shows clean bands post-restriction digest and before any DNA purification. After cloning and amplification on the bench, or with the BioXp™ 3200 system, the final DNA product can be digested to linear form and is then ready for downstream applications, negating the need for traditional E. coli transformation and plasmid purification. RapidAMP™ technology has been used successfully for transfection (lipofection and electroporation) and in vitro transcription.
Vmax™ X2 competent cells are engineered to retain the benefits of traditional bacterial protein expression systems — low cost, easy to grow, high transformation efficiency, and compatible with plasmids and antibiotics already in widespread use.
With an accelerated doubling time, Vmax™ X2 cells shorten protein expression workflows by one day and generate more soluble protein in less time than E. coli.
Additionally, Vmax™ X2 cells naturally have low immunogenicity with endotoxin levels 100 times lower than E. coli, making them an ideal host system for protein purification.
Strain type: T7 Expression Strain
Genotype: Single 50μl rxn per tube
Tube format: Single 50μl rxn per tube
Transformation efficiency: 10^7 CFU/μg DNA
Positive controls are useful as quality control measures for the verification and validation of both next-generation sequencing (NGS) and reverse transcriptase-polymerase chain reaction (RT-PCR) diagnostic assays.
Codex DNA offers synthetic SARS-CoV-2 RNA controls and supports collaborators with materials to develop their own.
The only next-generation bacterial expression system. Codex DNA offers a SARS-CoV-2 full-length synthetic genome (Wuhan-Hu-1 strain; GenBank number MN908947.3) cloned in a bacterial artificial chromosome that can be leveraged for the development of vaccines, therapeutics, and diagnostics. Genome modifications and editing are available.
Rapidly design and generate antibody libraries for the development of therapeutics using the BioXp™ system.
