The Essence of Metabolic Phenotyping - Accurate Metabolite Measurement
Accuracy and reproducibility of results are essential for advancing biomedical research. The Targeted Metabolomics Kits of Biocrates provide these benefits. They enable researchers to obtain quantitative information on up to hundreds of metabolites, with high accuracy, as well as reproducibility over time and between laboratories. The kits rely on thoroughly validated, quality controlled analytical methods which are optimized for their purpose, and the LC-MS instruments they are applied on. Comprehensive and reliable information about the metabolic phenotype of a study subject can be obtained in just a few minutes per sample, requiring very small sample volumes.
Product List
AbsoluteIDQ p180 Kit
Thanks to modern analytical instrumentation, the research discipline of metabolomics is constantly and considerably growing. Researchers may obtain large amounts of information about the metabolism of an organism from a single sample. However, no single analytical method can cover the whole metabolome. Methods that reliably and quickly provide meaningful results are required to harness the full potential of metabolomics.
The AbsoluteIDQ® p180 Kit is designed to address these challenges. It provides a simple and fast research tool for basic, clinical, and epidemiological researchers. The kit supports scientists in confidently obtaining detailed knowledge about the metabolic phenotype of their study subjects. Hundreds of published articles demonstrate the successful application of the kit, making it the most frequently chosen metabolomics approach of its kind. The AbsoluteIDQ® p180 Kit covers carefully selected metabolic pathways, using triple quadrupole mass spectrometry.
In total, up to 188 metabolites from 6 compound classes can be analyzed using a wide range of triple quadrupole mass spectrometry platforms:
- Hexoses
- Amino Acids
- Biogenic Amines
- Acylcarnitines
- Glycerophospholipids
- Sphingolipids
Features:
- High throughput: Analysis of up to up to 500 samples per week, making the kit suitable for use in large clinical and epidemiological cohorts.
- Reproducible: The kit has proven superior reproducibility in an international ring trial. (Siskos et al., Anal. Chem., 2017, DOI: 10.1021/acs.analchem.6b02930).
- Translatable: The kit has been validated for human plasma, and applied in a vast number of matrices from more than a dozen species. Hundreds of published articles make it easy to learn from findings from related research applications.
- Small sample volume: Just 10 µL of biofluid needed, allowing you to retain most of your precious samples for other analyses. Contact us for information about the applicability of other matrices.
Kit Information
Sample Preparation Guidelines
AbsoluteIDQ p400 HR Kit
Metabolomics is a powerful technology that can be universally applied in biomedical research. Standardised and reproducible quantification of a wide range of metabolites in your samples can be an important step towards a successful application of Metabolomics to your research project.
The AbsoluteIDQ® p400 HR Kit is a complete solution for broad lipid and metabolic profiling on high resolution, accurate mass (HRAM) Q ExactiveTM mass spectrometers. It provides quantification of a large range of analytes, with high inter-laboratory, inter-instrument and longitudinal reproducibility.
The kit covers up to 408 metabolites from 11 metabolite classes, which are known to be relevant in a multitude of pathophysiological processes:
Small Molecules:
- Amino Acids
- Biogenic Amines
- Hexoses
Polar Lipids:
- Phosphatidylcholines
- Lysophosphatidylcholines
- Sphingomyelins
- Ceramides
Neutral Lipids:
- Acylcarnitines
- Diglycerides
- Triglycerides
- Cholesteryl Esters
Highlights
- Quantification of up to 408 metabolites on Thermo ScientificTM Q ExactiveTM mass spectrometers: The AbsoluteIDQ® p400 HR Kit provides quantitative analysis of up to 365 lipids and 43 small molecules
- Ready-to-use: No method development required, method is established in your laboratory within one day.
- High throughput & minimal sample volume: Up to 250 samples per week; only 10 µL of human plasma required (other matrices tested).
- Simple, guided, automated workflow: Workflow manager MetIDQ® included for enhanced process safety; automated technical validation
- Superior data quality: The kit ensures batch-to-batch and inter-laboratory comparability.
Detailed kit information
Sample Preparation Guidelines
AbsoluteIDQ Stero17 Kit
Steroid hormones exert multiple biological functions. For example, they are involved in regulating glucose and mineral levels, as well as cell growth and proliferation. Steroid hormones are metabolites synthesised from cholesterol and primarily produced in the adrenal glands and gonads. As compared to other techniques, mass spectrometry offers the opportunity to quantify multiple steroid hormones in a single run with a higher accuracy.
The AbsoluteIDQ® Stero17 Kit provides a comprehensive solution for steroid hormone profiling. From a single serum sample, the kit enables the quantification of up to 17 steroid hormones from five steroid hormone classes:
- Progestagens
- Glucocorticoids
- Mineralocorticoids
- Androgens
- Estrogens
Highlights
- Comprehensive: Quantitative profiling of 17 steroid hormones, making the kit ideal for comprehensive analysis of steroid hormone metabolism in research endeavors.
- Reproducible: The kit has repeatedly proven to provide highly accurate results in ring trials by DGKL (German United Society for Clinical Chemistry and Laboratory Medicine).
- Standardized: The kit includes standardized reagents, as well as validated protocols for sample preparation and mass spectrometric analysis.
- High throughput: Up to 500 biofluid samples per LC-MS instrument per week (other matrices have successfully been tested).
Detailed Kit Information
Sample Preparation Guidelines
AbsoluteIDQ Steroid Reagents
MS-based steroid hormone analysis has become customizable: scientists are free to use their method of choice as well as to decide which of the 17 analytes they wish to target.
BIOCRATES AbsoluteIDQ® Steroid Reagents permit simultaneous reliable quantification of as many as 17 steroids, using LC-MS/MS. The reagents are designed for use in combination with "home-brewed" LC-MS methods.
AbsoluteIDQ® Steroid Calibrators
Are designed for quantitative and simultaneous determination of up to 17 different steroid hormones
AbsoluteIDQ® Steroid Quality Controls
Are designed for quantitative determination of up to 17 different steroid hormones.
AbsoluteIDQ® Steroid Internal Standards
Are a mixture of 15 labeled steroid hormones designed for internal calibration of LC-MS/MS-based steroid hormone quantification.
AbsoluteIDQ® Steroid Internal Standards
Contains 17 steroids without matrix or internal standards. The test mix serves to check the performance of your LC-MS/MS system.
Biocrates Bile Acids Kit
Bile acids are known to help absorb dietary fats and fat soluble vitamins. Beyond this core function, bile acids exert many additional biological functions via receptor signaling, including the nuclear receptor farnesoid X receptor (FXR). Since bile acids are modulated by bacteria in the intestines, bile acids are also of great interest in studies related to the role of gut microbiota in health and disease.
The Biocrates® Bile Acids Kit uses the sensitive and specific technology of mass spectrometry to provide quantitative information about up to 20 bile acids.
The compounds covered by the kit include all four types of bile acids:
- Primary bile acids: produced by the liver
- Primary conjugated bile acids: primary bile acids transformed by liver enzymes
- Secondary unconjugated bile acids: primary bile acids transformed by gut bacteria
- Secondary conjugated bile acids: secondary unconjugated bile acids transformed by gut bacteria and liver enzymes
Highlights
- Ready-to-use: The kit includes standardized reagents, as well as validated methods for sample preparation and mass spectrometric analysis.
- High throughput: One kit analyses up to 80 samples within 12 hours.
- Proven reproducibility: The kit has proven to provide excellent inter-laboratory comparability in an international ring trial (Pham et al., J Appl Lab Med., 2016, DOI: 10.1373/jalm.2016.020537).
- Small sample volume: Just 10-20 µL of serum or plasma needed (other matrices have successfully been tested).
Detailed Kit Information
Sample Preparation Guidelines
Thanks to modern analytical instrumentation, the research discipline of metabolomics is constantly and considerably growing. Researchers may obtain large amounts of information about the metabolism of an organism from a single sample. However, no single analytical method can cover the whole metabolome. Methods that reliably and quickly provide meaningful results are required to harness the full potential of metabolomics.
Metabolomics is a powerful technology that can be universally applied in biomedical research. Standardised and reproducible quantification of a wide range of metabolites in your samples can be an important step towards a successful application of Metabolomics to your research project.
Steroid hormones exert multiple biological functions. For example, they are involved in regulating glucose and mineral levels, as well as cell growth and proliferation. Steroid hormones are metabolites synthesised from cholesterol and primarily produced in the adrenal glands and gonads. As compared to other techniques, mass spectrometry offers the opportunity to quantify multiple steroid hormones in a single run with a higher accuracy.
MS-based steroid hormone analysis has become customizable: scientists are free to use their method of choice as well as to decide which of the 17 analytes they wish to target.
Are designed for quantitative and simultaneous determination of up to 17 different steroid hormones
Are designed for quantitative determination of up to 17 different steroid hormones.
Are a mixture of 15 labeled steroid hormones designed for internal calibration of LC-MS/MS-based steroid hormone quantification.
Contains 17 steroids without matrix or internal standards. The test mix serves to check the performance of your LC-MS/MS system.
Bile acids are known to help absorb dietary fats and fat soluble vitamins. Beyond this core function, bile acids exert many additional biological functions via receptor signaling, including the nuclear receptor farnesoid X receptor (FXR). Since bile acids are modulated by bacteria in the intestines, bile acids are also of great interest in studies related to the role of gut microbiota in health and disease.
