PacBio provides complete workflows and all the consumable components you need to perform Single Molecule, Real-Time (SMRT) Sequencing. Regardless of project size, the consumables promote customization while maintaining cost-effectiveness. Due to the flexibility of the PacBio sequencing platforms, these components support a variety of workflow steps and research goals. Long, accurate sequence reads are only a few steps away.
DNA (genomic DNA or amplicons) and cDNA camples are converted into a primer-annealed SMRTbell library format. May be performed manually at the benchtop or by an automated liquid-handling system.
Primer-annealed SMRTbell tamplates are bound to a DNA polymerase in preparation for sequencing. Polymerase-bound SMRTbell templates are annealed to magnetic beads for loading onto the PacBio systems.
SMRT Cells and DNA sequencing reagents required for on-instrument sequencing.
Muti-well plates, septa, and tips specifically designed and optimised for use with the sequel system.
DNA (gDNA or amplicons) and cDNA samples are converted into a primer-annealed SMRTbell library format. May be performed manually at the benchtop or by an automated liquid-handling system.
Primer-annealed SMRTbell templates are bound to a DNA polumerase in preparation for sequencing. Polymerase-bound SMRTbell templates are annealed to magnetic beads for loading onto the PacBio systems.
SMRT Cells and DNA sequencing reagents required for on-instrument sequencing.
Multi-well plates, septa, and tips specifically designed and optimised for use with the PacBio RS II System.
Target enrichment through a solution-based capture system enables enrichment of defined regions of interest up to several Mb in size. Roche NimbleGen's SeqCap EZ enrichment technology, combined with PacBio's SMRT Sequencing, has demonstrated the ability to use 6 kb fragments and is applicable to practically any type of SeqCap EZ panel. They have demonstrated efficient enrichment for the Major Histocompatiblity Complex and a comprehensive cancer gene panel. On average, 61% of the reads were on target and an average enrichment factor of 1300x was obtained. With PacBio long reads, heterozygous SNPs were used to phase the reads and generate haplotypes.
Features
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1000-fold enrichment.
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Full-gene capture - exonic and intronic regions.
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Phase variants to obtain haplotypes.
Direct PCR amplification of a specific region is a powerful technique for performing targeted sequencing. When combined with multiplexing capabilities, PCR amplification is an efficient approach to studying many samples. PacBio offers two barcoding strategies for multiplexing. In the first approach, barcodes are added to the end of the amplicon during PCR, and the standard SMRTbell adapter is subsequently ligated onto the barcoded amplicon.
Features
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Validated with amplicons up to 10kb in length.
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Formatted to allow multiplexing from 2 to 96 samples.
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Separate workflows for validated or changeable primer designs.
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Decreased template preparation cost up to 90-fold.
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Decreased time-to-result for template preparation up to 50%.
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Configured for automated liquid handlers.
