On this page
Protein Analysis
Protein Analysis & Purification
Active and purified proteins play crucial roles in nearly all biological processes, especially in the fields of functional and structural analysis, assay development, and drug candidate discovery. GenScript offers a broad spectrum of protein products, including protein expression vectors, protein isolation and purification resins and magnetic beads, Precast PAGE Gels, protein markers, eStain™ L1 Protein Staining System, ONE-HOUR Western Detection Kits, and a wide selection of Catalog Proteins, giving you a direct path to successful protein research projects.
Protein Purification Resins
Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. Among various protein purification technologies, affinity chromatography is absolutely the most important and powerful method since it can offer high selectivity, high resolution, and high capacity for target proteins. GenScript provides a variety of simple-to-use affinity purification resins for batch/gravity purification, allowing for efficient, convenient and reliable separation of proteins and antibodies from your crude sample for further applications.
Key Features
- Excellent affinity chromatography media for protein and antibody purification
- Fast and simple procedures for batch or gravity-flow operation
- High binding capacity with ultra low nonspecific binding
- Compatible with a variety of sample formats and buffer additives
- Boosted cost-effectiveness due to reusability for several times
Applications
- Antibody purification
- His and GST tagged protein purification
- Purification of streptavidin, avidin or their conjugates
- Purification of biotinylated protein, antibody, lectin etc
Top of page
Precast Gels
High Resolution & Robust Separation
- Large well volume – Up to 80 µl for diluted samples
- High resolution – Even, sharp bands, guaranteed lot-lot consistency
- Long shelf life – Up to 12 months at 2-8℃
- Cost effective – 30-50% price reduction compared to other major competitors
- Compatible cassette design – Fits all popular mini-gel tanks
GenScript's Bis-Tris precast gel series are high performance polyacrylamide gels that are designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability. They also run at neutral pH which minimizes protein modification compared to Tris-glycine gels. GenScript's gel series include-SurePAGE™ Gels which are the premium, higher resolution and reproducibility gels, and ExpressPlus, good quality and very cost-effective gels
Top of page
eStain™ L1 Protein Staining System
10-min Protein Gel Staining with ultra-sensitivity and simplicity
- Versatile: Gel staining with Coomasie Blue (offered by GenScript) or membrane staining with Ponceau S solution
- Fast: Stain and destain in less than 10 min
- Automatic: Reagents are pumped in/out automatically
- Sensitive: sharply stained protein bands and sensitivity ≥ 12.5 ng
- Consistent: Results are highly repeatable
- Efficient: Simultaneously stain/destain two mini gels or membranes
eStain is a highly efficient protein PAGE gel staining system, which uses Coomassie Brilliant Blue and a patented protein staining technology developed by Genscript. eStain staining system integrates the traditional three steps of fixing-staining-destaining into one and can stain/destain two protein PAGE gels simultaneously in 10 minutes or less. eStain works with all types of precast as well as homemade PAGE gels. Compared with the conventional staining methods, gels stained by eStain have crisp blue bands with minimum or no background. eStain also offers superior sensitivity compared to conventional methods and can detect as low as 12.5 ng of protein.
Top of page
Western Blotting Transfer System
eBlot™ L1 Fast Wet Transfer System for Mini Gels
eBlot™ L1 wet protein transfer system is a highly efficient western blotting transfer system that uses a novel technology (patent pending) developed by GenScript. eBlot™ L1 combines the high reproducibility and efficiency of the traditional wet transfer with the speed and convenience of other fast transfer systems. The device allows efficient blot transfer of small, medium and large molecular weight proteins within 9-17 minutes.
Benefits of eBlot™ L1
- Comprehensive: Outstanding performance for small, medium and large proteins
- Convenience: Simultaneously transfer two mini gels
- Compatible: Highly compatible with different types of precast or homemade gels
- Simple: Easy to assemble, one button operation and customizable programs available
The eBlot™ L1 system is for wet electroblotting of proteins from mini gels onto PVDF or nitrocellulose membranes. Enjoy a top-quality and rapid protein transfer eBlot™ L1 brings you!
Top of page
eZwest Lite
eZwest™ Lite is an automated western blotting device providing an easy and simple solution in your western blotting workflow. After proteins are transferred onto the membrane, load the membrane into our eZwest™ Lite device for automated blocking, incubation, and washing cycles. The user only needs to prepare the required reagents, run the customized program, and eZwest™ Lite will take care of the rest. Once the program is finished, the user can collect the results and immediately proceed to detection and analysis steps. eZwest™ Lite is easy to use and has minimal footprint.
Advantages
- Consistent Results: Fully automated operation ensuring consistency every time
- Antibody Saving: Automatically recover primary antibody for re-use
- Programmable: Easily customization program for protocol need
- Completely Hands-off: Load your reagents and Go
Simplify & Save Hours of Labor in Your Western Blot Workflow
Top of page
GenScript Magnetic Beads
Magnetic Beads for protein and antibody purification
To enable fast and efficient purification of antibody and epitope-tagged proteins, GenScript provides super paramagnetic MagBeads. GenScript MagBeads are coated with recombinant protein ligands that enable highly specific affinity binding to antibodies and epitope-tagged proteins. The magnetic protein purification method allows for protein purification directly from cell lysates, thus eliminating the need for centrifugation steps and simplifying protein purification. The high binding capacity, specificity of binding, and the superfast magnetic response makes GenScript MagBeads ideal for applications requiring fast turnaround time and large number of samples.
Why choose magnetic beads?
The advantage of using magnetic beads is that it eliminate steps like centrifugation or filtration, which are involved in the traditional methods of protein purification. This can significantly save your time and labor.
Key Features
- Quick, easy and convenient magnetic separation procedures
- High binding capacity
- Reproducible results due to highly consistent bead size
- Extremely gentle sample handling minimizing protein degradation
Applications
- Antibody purification
- Purification of tagged proteins e.g. 6x His, GST, biotin etc.
- High throughput protein and antibody purification
Top of page
Protein Standards
GenScript offers a wide selection of Protein molecular weight standards for SDS-PAGE and Western blot, including prestained, unstained, peptide, Western-viewable, and other standards. The Pre-stained Protein Standards consist of colored bands for easy identification and allow you to monitor the progress of an electrophoretic run. The Unstained Protein Standards provide bands that are unmodified by the presence of dye, for accurate molecular weight estimation of SDS-PAGE. Available in convenient package sizes, all standards have undergone stringent QC to assure consistent performance to provide the sharper bands in the broader molelular weight range available.
Key Features
- Comprehensive Selection: a variety of standards available for SDS-PAGE and Western blotting.
- Broad Molecular Weight Range: low, middle and broad MW range standards suitable for all practical needs.
- Easy Identification: clean, sharp protein bands for accurate MW estimation and assessment of transfer efficiency.
Top of page
CRISPR Products
Complexing a CRISPR nuclease with the guide RNA, commonly referred to as the ribonucleoprotein (RNP) complex, has been shown to be the leading CRISPR gene editing mechanism. RNP complex provides scientists with enhanced gene editing performance, while reducing common challenges observed with other delivery systems, including viral- and plasmid-based. GenCRISPR™ nuclease offerings provide scientists with RNA and DNA editing solutions with proven performance including Cas9, eSpCas9, Cas9-EGFP, D10A Nickase, Cas12a and Cas13a.
Top of page
GenCRISPR Ultra Nucleases
The GenCRISPR Ultra product line is a comprehensive collection of Cas-based nucleases with enhanced gene editing performance. GenScript's Ultra product line supports progression of gene-editing research through two quality-controlled product tiers: research use only (RUO) and GMP compliant. The Ultra RUO products provide cost-effective solutions for basic research applications. With stringent quality controls, ISO9001, ISO13485 and GMP manufacturing guidelines, the GMP products are ideal solutions for pre-clinical and clinical applications.
Benefits
Nuclease Grades
Top of page
CRISPR systems are powerful gene editing tools with proven success across single and multi-cellular organisms. GenScript's GenCRISPRTM portfolio provides scientists with comprehensive solutions for every step of their gene editing workflow.
GenCRISPR™ Kits
The ready-to-use GenCRISPR™ kits simplify Cas-mediated gene-editing workflows. From identifying functional gRNAs before transformation to evaluating the efficiency of gene editing experiments, the GenCRISPR kits support gene editing research every step of the workflow.
GenCRISPR™ Antibodies
GenScript offers Cas9 and Cas12a (CpF1)-specific antibodies to detect enzyme expression.
Top of page
Antibodies
Reliable and well-selected antibodies are among the most effective tools available to researchers when it comes to identifying and locating specific proteins. Increasingly, antibodies have been employed to directly diagnose and treat diseases. GenScript provides a broad range of high-quality Primary and Secondary antibodies such as Anti-Epitope tag, Immune Checkpoint, Cas 9 and more. These products can be applied to all areas of biological research and drug discovery. All GenScript's antibodies are thoroughly tested to ensure the highest level of specificity, sensitivity, and reproducibility.
MonoRab™ Rabbit Monoclonal Antibodies
Distinct advantages of rabbit monoclonal antibodies (mAbs) have led to their increasing applications in all areas of life sciences, from basic research to diagnostics and therapeutics. GenScript's MonoRab™ technology generates high quality rabbit monoclonal antibodies. Our proprietary hetero-hybridoma methodology includes an optimized fusion partner to improve overall fusion efficiency while utilizing the rabbit's natural ability to generate high specificity, high affinity monoclonal antibody products against a wide range of difficult targets such as small molecules, Ab drugs, and even antigens non-immunogenic in mice.
Why choose?
Top of page
Epitope Tag Antibody Family
An epitope is an antigenic determinant, a biological structure or sequence to which an antibody binds. Recombinant DNA technology allows scientific researchers to construct fusion proteins which express the gene of interest and have an epitope tag. The use of epitope tags eliminates the additional step of using a different antibody for each newly purified protein. The most commonly used epitope tags include His (6-Histidine), HA (Hemagglutinin), cMyc, GST, and Flag (DYKDDDDK) tag. GenScript offers highly specific antibodies to all of these epitope tags. Genscript's tagged antibodies are manufactured under extensive quality control to ensure optimal sensitivity and consistent high performance.GenScript THE™ elite antibodies, including Tag & Cell Marker Antibodies, Loading Control Antibodies and Assay Antibodies, have the best known performance in the industry. Now MonoRab™ Rabbit Monoclonal series of anti-tag antibodies are also available!
Top of page
Primary Antibodies
Primary antibodies recognize and bind to unique epitopes with high affinity and specificity across a broad spectrum of biomolecules. They exist as high specificity monoclonal antibodies and/or as polyclonal antibodies. These antibodies are used for detecting specific biomolecules or measuring changes. GenScript offers over 1000 highly specific monoclonal and polyclonal primary antibodies, covering major areas of life science research, such as H1N1 Antibodies, Neuroscience Antibodies for Alzheimer's Parkinson's and Huntington's disease, Cytokine and Growth Factor Antibodies, Control Antibodies including isotype and loading control antibodies, Cell Marker Antibodies, and more. These primary antibodies are validated for multiple applications, including Sandwich ELISA and western blot. GenScript also offers more than 200 cytokines, chemokines and growth factors, for your cell signaling and stem cell research demands with key features such as low endotoxin levels (<1.0 EU/μg) and high purity ( >95% ).
Top of page
Immune Checkpoint Antibodies
Immune checkpoint-blocking antibodies that activate the immune system to fight cancer are becoming an important method of cancer treatment. CTLA-4 was the first immune checkpoint target and ipilimumab, an anti CTLA-4 antibody drug, was approved in 2011 for clinical use. The PD-1 receptor and its ligand PD-L1 have shown great promise as therapeutic targets in a variety of malignancies. The combined- target immunotherapy may have a higher level of effect in the future. To facilitate this hot immunotherapy research field, GenScript provides immune checkpoint antibodies and immune checkpoint proteins for many kinds of applications. Products for more targets are coming in succession.
Top of page
MonoRab™ Anti-Camelid VHH Antibody Products
Rabbit anti-camelid VHH monoclonal antibody series generated by Genscript's proprietary rabbit-mouse hetero-hybridoma technology MonoRab provide perfect secondary antibody tools for researchers to identify target-specific sdAbs.
Top of page
Anti-idiotype Antibody Products
Top of page
Secondary Antibodies
A primary antibody's Fc domain is constant within the same animal species, therefore, a given secondary antibody can be used with any primary antibody of the same isotype and target species. This reduces the cost by labeling only one type of secondary antibody. In addition, secondary antibodies offer increased sensitivity through the signal amplification. At GenScript, secondary antibodies are purified from the serum of several hosts, including goat, donkey, mouse, rabbit, chicken and sheep. Secondary antibodies are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. The conjugated secondary antibodies can be used for various immunology analysis and protein assays. GenScript also offers over 1000 highly specific monoclonal and polyclonal primary antibodies. Click here to look for primary antibodies for your protein of interest.
Top of page
Phospho-Specific Antibodies
Phosphorylation/dephosphorylation is one of the major signaling mechanisms used to modulate the functional properties of proteins involved in gene expression, cell adhesion, cell proliferation, cell differentiation, and the cell cycle. The determination of the state of phosphorylation is highly important for studying the function of a given protein. Phospho-specific antibodies, which can discriminate between the phosphorylated and non-phosphorylated forms, have emerged as demonstrated assets in the study of signal transduction pathways. These novel tools enable qualitative and quantitative detection of phosphorylated proteins without the risks associated with using radioactivity. GenScript's phospho-specific antibodies are affinity-purified and do not cross react with non-phosphorylated forms of proteins. Phospho-specific antibodies detect only the phosphorylated forms of proteins in a complex protein mixture within cells. They recognize the phosphorylated amino acids in the context of the surrounding amino acid sequence. Using a combination of pan-antibody (that recognizes both phosphorylated and non-phosphorylated forms of the protein) and phospho-specific antibody, it is possible to assess the degree of phosphorylation for any given protein. GenScript offers phospho-specific/pan antibody pairs that are suitable for many tasks, including Western blot analysis, ELISA, and immunocytochemical assays. Genscript also provide blocking peptides for most of their phospho-specific and pan antibodies. These peptides can be used to confirm that the observed signal is due to the antibody's interaction with target antigen, as opposed to non-specific binding.
Top of page
Cytokines
Cytokines, a category of immunoactive proteins, act as humoral regulators which modulate the functional activities of individual cells and tissues at nano to picomolar concentrations under normal or pathological conditions. The cytokine family consists mainly of small, water-soluble proteins and glycoproteins with a molecular mass between 8 and 30 kDa. These proteins are secreted by immune cells and include interleukins, interferons and certain growth factors. GenScript offers a comprehensive catalog of recombinant cytokines with excellent lot-to-lot consistency, superior activity and significantly low endotoxin levels. GenScript's goal is to provide scientists quick and easy access to research tools that advance innovation and discovery.
Top of page
Recombinant Proteins
Purified recombinant proteins are vital tools for signal transduction analysis, assay development and drug discovery. GenScript offer a wide variety of recombinant proteins to provide researchers a direct path to scientific innovation and success. With GenScript's expertise and support, you have a knowledgeable committed partner to help advance your research and reach your goals.
Top of page
Enzymes & Inhibitors
Enzymes which serve a variety of functions inside living organisms are widely used in academic research and industrial production. Almost all processes in a biological cell require the presence of enzymes to occur at significant rates. Through years of experience, GenScript has developed many widely-used enzyme products to fit the needs of rapidly advancing research fields.
Top of page
Chemokines
Chemokines are a family of small cytokines, or proteins secreted by cells, with a molecular mass between 8 and 10 kDa. Members of the chemokine family are divided into four groups depending on the location of their first two cysteine residues. The major role of chemokines is to act as a chemoattractant to guide the migration of cells. GenScript offers a comprehensive catalog of chemokine proteins with excellent lot-to-lot consistency, superior activity and significantly low endotoxin levels. Genscript's goal is to provide scientists quick and easy access to research tools that advance innovation and discovery.
Top of page
Hormones
Hormones are produced by specialized glands and can function at sites distant from where they are released. They act as chemical messengers that transport signals from one cell to another.
Top of page
Growth Factors
Growth factors are known for their ability to enhance cell proliferation, cell growth and cellular differentiation by regulating tissue morphogenesis, angiogenesis and neurite outgrowth. Growth factors typically act as signaling molecules between cells which play an important role in regulating a variety of cellular processes. Their activities are mediated through binding to transmembrane receptors. The regulation of some growth factors and their receptors are also involved in tumor formation.
Top of page
Researchers have been developing diagnostic tools, potential vaccines and drugs to fight viruses. The transmission of a newly identified coronavirus SARS-CoV-2 (formerly 2019-nCoV) is very strong. GenScript now offers comprehensive portfolio of off the shelf products for SARS-CoV-2 research . All the proteins for COVID-19 research serve as excellent antigens in ELISA and Western Blot and in the rapid detection of relative virus seroconverters with optimal specificity.
Top of page
Neurotrophins
Neurotrophins are a family of proteins that induce the survival, development, and function of neurons. They belong to a class of growth factors, secreted proteins that are capable of signaling particular cells to survive, differentiate, or grow. Growth factors such as neurotrophins that promote the survival of neurons are known as neurotrophic factors. Neurotrophic factors are secreted by target tissue and act by preventing the associated neuron from initiating programmed cell death - thus allowing the neurons to survive. Neurotrophins also induce differentiation of progenitor cells, to form neurons.
Top of page
Human Hematopoietic Stem Cell Expansion Cytokine Bundle
Human Hematopoietic Stem Cell Expansion Cytokine Bundle contains the key components required for ex vivo Human Hematopoietic Stem Cell Expansion.
Key Features
- The manufacture process of cytokines is carried out according to GLP guidelines.
- Exceptionally low endotoxin level, less than 0.2 EU/µg – less than most other suppliers' standards (1 EU/µg).
- High purity determined by SDS-PAGE and HPLC analysis.
- High biological activity and functionality.
- Excellent lot-to-lot consistency.
- Economical package to prevent loss of materials and reduce experiment cost due to possible contamination.
- Competitive prices compared with other suppliers'.
Top of page
Immune Checkpoint Proteins
The clinical study of immune checkpoint proteins achieved great success, making immunotherapy a promising method to treat cancer. Immune checkpoints are involved in a series of immunomodulatory pathways that can either co-stimulate or co-inhibit the immune response. Co-stimulatory immune checkpoints enhance the body's immune response against pathogens. Co-inhibitory immune-checkpoints can negatively regulate T cell's activity to protect against pro-inflammatory reactions. Targets, such as PD-1, PD-L1, B7, CD28, TIM and CD226, are very hot in cancer treatment. GenScript immune checkpoint recombinant proteins passed a variety of activity testing and are featured with high purity and batch to batch stability. They are used as immunogens for the production of GenScript immune checkpoint antibodies.
Application
- Modulate immune responses
- Identify binding partners
- Discover novel drugs
- Antibody production
Top of page
Molecular Biology Reagents
GenScript's high-quality molecular biology reagents provide laboratories with consistent, robust and accurate results. The comprehensive offerings cover a broad range of PCR reagents, cloning kits and vectors.
PCR Reagents
GenScript's advanced technology has led to the invention of high-quality PCR reagents , including the recombinant form of regular Taq DNA Polymerases , high-stability Taq DNA Polymerases , dNTPs, Master Mixes, and more. They simply provide you with instant access to high throughput PCR automation, offering cost-effective solution and satisfactory results.
Applications
- PCR
- 3'A-tailing of blunt ends
|
- Primer extension
- DNA sequencing
|
Top of page
Green PCR Reagents
GenScript's advanced high-stability technology has led to the invention of the new generation of PCR reagents—Green PCR Reagents. The Green series of reagents, from the proprietary Taq, dNTP, to PCR mixtures , are stable for one month if stored at room temperature, or for over six months if stored at 4°C. The high-stability feature comes with increased half-life of active enzymes at high temperatures, which results in more amplification cycles and higher yield. With no freeze-thaw cycles, green PCR reagents provide you an instant access to high-throughput PCR automation. In addition, GenScript's Green PCR Reagents can help preserve energy associated with storage and shipping.
Key Features
-
S table at room temperature
-
Enhanced yields with longer enzyme half-life
-
Stop refrigerating and save energy
Top of page
GenBuilder™ DNA Assembly
GenScript's GenBuilder™ DNA Assembly technology takes recombination-based cloning to the next level. Pushing the bounds of innovation, the GenBuilder™ technology allows for seamless fusion of one to twelve gene fragments with the highest efficiency. Whether using fragments longer than 5 kb, unpurified PCR products, single stranded DNA inserts, or have only fifteen minutes to complete the cloning reaction- the GenBuilder™ DNA Assembly technology helps you build your constructs quickly and accurately.
Top of page
Cloning Vector
GenScript offers four unique expression vectors with enhanced promoters for efficient expression, selection, and analysis of recombinant proteins
Top of page
Stable Cell Lines
Stable cell lines are important in vitro cellular tools. Over-expressing cell lines are needed to screen for hits, while functional cell lines are needed for "lead" identification. Since 2009, GenScript has successfully generated more than 200 stable cell lines for research in this field. The Stable Cell Line Catalog Products are generated using antibiotic selection system and limited dilution technology to ensure single clone products. The target genes of expression are tag-free to allow for label-free investigation.
Top of page
ACE2 Stable Cell Lines for SARS-CoV-2 Investigation
The outbreak of coronavirus disease 2019 (COVID19) has posed a global threat to public health. A novel coronavirus, SARS-coronavirus 2 (SARS-CoV-2), has been identified as the etiologic agent of this disease. Spike protein of SARS-CoV-2 (previously named 2019-nCoV) is composed of a S1 domain and S2 domain. The S1 domain contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. It is believed that SARS-CoV-2 Spike Protein (RBD) has potential value for the diagnosis of the virus. Cell entry of coronavirus depends on the spike (S) protein binding to a cellular receptor and priming by a cellular protease. Recent studies demonstrate that angiotensin-converting enzyme 2 (ACE2) serves as the cell surface receptor to bind S protein in SARS-CoV-2 and facilitates entry of these coronaviruses into the cell. GenScript have developed single clone stable cell lines with high expression of the key regulators of COVID19, SARS-CoV-2 Spike protein or human ACE2 (hACE2) as powerful cell line tools for drug discovery to treat COVID19.
Key features
Both HEK293/ACE2 and CHO-K1/ACE2 stable cell lines are generated with the features below:
- Single clone cell line
- Validated for stable hACE2 expression and S protein RBD binding
- Easy to use in binding assays or as immunogens
Applications
- Neutralization assays to screen antibodies or other molecules that can block pseudovirus or live virus infection
- Affinity analysis of antibody or other molecules to Spike protein
- In vitro cell models for investigating SARS-CoV-2 infection mechanism
Top of page
Immune Checkpoint Stable Cell Lines
Inhibitors of immune-checkpoint proteins, such as PD1, PD-L1, VISTA, Tim3 and Lag3, have indicated broad and diverse opportunities to increase antitumor immunity by enhancing T cell activity with the potential to produce durable clinical responses. To meet these needs, GenScript have developed stable cell line products expressing the most popular immune checkpoints to accelerate your immunotherapy discovery and clinical translation. All these stable cells are developed for screening high affinity antibodies against immune checkpoint proteins.
Key features
- High protein expression at the cell surface validated by FACS
- Stable cell lines for increased reliability and reproducibility (over 15 passages)
- Ready to use hot targets, saving 3-5 months
Top of page
Fc Receptor Stable Cell Lines
Fc receptors (FcRs) are membrane proteins that bind with antibody Fc regions and help in regulating the body's immune response. A variety of Fc receptors (Figure 1) selectively bind the Fc region of specific antibodies (Table 1) . When these antibodies are attached to disease-causing pathogens or infected cells, the Fc receptors can mediate their destruction by antibody-dependent cellular phagocytosis (ADCP) or antibody-dependent cell-mediated cytotoxicity (ADCC, Figure 2 ). The Fc region can also interact with complement proteins to induce complement-dependent cytotoxicity (CDC). The interaction of Fc receptors with complement proteins results in a wide range of modulated immune responses. Due to the central role of Fc receptors in regulating antibody function, binding kinetics of new antibodies may be tested using Fc receptor-expressing cell lines when developing antibody-based drugs and therapies.
Fc gamma receptors (FcγRs) (Figure 1) bind Immunoglobulin G antibodies (IgGs), and can enhance or inhibit IgG-based immune responses [1-5]. In the field of tumor biology and development of antibody therapies for cancer, the ADCC function of FcγRs has gained prominence. ADCC is initiated when FcγRs expressed on the surface of natural killer (NK) or other types of effector cells such as macrophages recognize and bind with the Fc domain of antibodies which are attached to the surface of tumor cells. The interaction with FcγRs (most often FcγRIIIA) with these bound antibodies releases perforins and granzymes from the effector cells, resulting in the lysis of the tumor cells (Figure 2). FcγRs make good targets for antibody-based therapies for cancers as they can assist in the destruction of diseased cells by ADCC [6]. Cell lines expressing FcγRs are often used to determine effectiveness of antibody drugs developed to treat various types of cancers and autoimmune diseases.
Some FcγRs have several polymorphic variants due to point mutations. The FcγR variant expressed by an individual can affect their chance of developing certain diseases such as SARS and ulcerative colitis, as well as the disease intensity (Table 1) [7-26]. FcγR variants can also determine a patient's response to antibody-based drugs and therapies. Therefore, cell lines expressing FcγR polymorphisms are useful for developing personalized medicine.
Another type of Fc receptor, the neonatal Fc receptor (FcRn) is indicated in increasing the stability of antibodies and serum proteins such as albumins [27]. FcRn binds IgGs and albumins in a pH-dependent mechanism and mediates their transcytosis across polarized membranes [5]. FcRn is present in a variety of cells, including placental cells which transport IgGs from the mother to the developing fetus [24]. Cell lines expressing FcRn are useful screening tools to study transcytosis and in vitro clearance of antibody-based drugs.
Product Highlights
- Single-clone cell lines for stable and consistent receptor expression on host cell surface.
- Polymorphic versions of popular FcγRs available for targeted drug development.
- Use of human receptors expressed in CHO-K1 host cell line for in vitro drug testing.
Applications
- Determination of binding affinity of antibody-based drug candidates.
- Determination of the half-life of antibody-based drug candidates.
- Determination of the drug candidate's mechanism of action in vitro.
- Research and development of personalized medicine.
Top of page
Fc Receptor Stable Cell Lines
GenScript offers a wide selection of GPCR stable cell lines. Proprietary codon optimization and vector design technologies enable GenScript to overcome low functional protein expression at the cell surface. All of the cell lines have been optimized for S/B ratio in calcium flux assays, FRET assays, or chemiluminescent assays.
Key features
- Gα15 expressing cells can be used directly for FLIPR analysis
- Stable cell lines for increased reliability and reproducibility
- Flexible functional readouts are available
Top of page
CytoSinct Magnetic Cell Separation
Efficient and gentle cell separation based on surface markers is essential for researching specific cell populations, and for developing life-saving cell and gene therapies.
GenScript has renowned expertise in developing magnetic bead products for bioseparation of various types of biomolecules. GenScript's novel CytoSinct™ Cell Separation Technology uses their perfected nanoparticle empowered immunomagnetic separation technology to isolate specific cell populations of interest, combining the specificity and sensitivity of antibody-based purification with the gentleness of nanoparticles and the ease and speed of magnetic separation.
The CytoSinct™ Cell Separation Manual Kit is composed of antigen specific paramagnetic Nanobeads, Columns, and magnetic Stands. The CytoSinct™ Nanobeads are nanometer-sized, biodegradable, easy-to-use, and enable highly efficient cell isolation. CytoSinct™ Columns amplify the magnetic field and enable efficient separation with minimal labeling using the small beads. The separated cells are compatible with most downstream applications including cell culture, activation, expansion, flow cytometry analysis and translational research. Armed with the flexibility and specificity of the CytoSinct™ Cell Separation technology, you can use a variety of starting materials. Start with PBMCs or leukapheresis products. Enrich your target cells in your sample or deplete them. For every experimental application, the CytoSinct™ Cell Separation technology can provide you with the separation results you require. Even more, CytoSinct™ Cell Separation Kit is compatible with other column-based cell separation platforms. Try this great tool to advance your research by simplifying your cell separation procedure.
Top of page
