InvivoGen provides life scientists with innovative research reagents. As experts in innate immunity and cell culture, they offer a large selection of products.

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Cell Culture Reagents

InvivoGen provides a wide range of high-quality reagents for tissue and cell culture applications, including mycoplasma detection, prevention and elimination, plus various antimicrobial agents, selective antibiotics, reporter detection kits, the transfection reagent LyoVec™, the transfection enhancer NATE™, and an endotoxin detection kit. These products are functionally validated and undergo strict quality control.

Practical Guide to understanding culture contamination - Download

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NEW MycoStrip - Mycoplasma detection strips

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PlasmoTest - Mycoplasma detection cells

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Cell Transfection

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Selective Antibiotics

InvivoGen is a leader in the production of selection antibiotics. InvivoGen's state-of-the-art facilities allow us to produce large quantities of high quality, endotoxin-free antibiotics with purity levels exceeding 95%.

Ready-to-use Cell Culture Tested Solutions - No weighing needed - InvivoGen's antibiotics are available in solution filtered to sterility for customer convenience and validated for cell culture usage.

An Excellent Choice of Antibiotics for Selection in Both Mammalian cells and E. coli - Matches up to the antibiotic resistance genes carried by InvivoGen plasmids, built for selection in both mammalian and bacteria cells.

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PRR ligands

InvivoGen provides an extensive set of tools to study the innate immune system, including a comprehensive collection of pathogen-associated molecular patterns (PAMPs).

PAMPS are recognized by pattern recognition receptors (PRRs). Five families of PRRs have been shown to initiate pro-inflammatory signaling pathways:

  1. Toll-like receptors (TLRs)
  2. NOD-like receptors (NLRs)
  3. RIG-I-like receptors (RLRs)
  4. C-type lectin receptors (CLRs)
  5. Cytosolic dsDNA sensors (CDSs)

Also some NLRs are involved in the formation of pro-inflammatory complexes called inflammasomes. InvivoGen also provide labelled ligands to activate or study these receptors, and offer diverse NF-κB and NFAT activators.

TLR & NLRs Pathway Poster CLR-RLR-CDS Pathway Poster Inflammasome Activation Poster

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Conjugatable PRR Ligands

Conjugatable PRR ligands are synthetic PAMPs that have been chemically modified to allow their attachment to a peptide/protein with a chemical linker. Their assembly generates a bioconjugate. Examples of promising therapeutic bioconjugates include immune-stimulating antibody conjugates (ISACs) and antigen-adjuvant conjugates (AACs). InvivoGen offers conjugatable ligands for PRRs that are expressed by dendritic cells and other antigen-presenting cells. The targeting of TLR7 or STING is of particular interest as it triggers the production of pro-inflammatory cytokines potentiating immune responses in cancer or pathogen-driven disease models.

To facilitate your research on ISACs and vaccination, InvivoGen has developed two classes of conjugatable ligands allowing:

•  Simple thioether conjugation: ready-to-use ligands featuring a thio-reactive maleimide, bypassing the need for linker design and ligand modification.

•  Flexible conjugation: ligands featuring an azido group, offering a flexible choice among commercially available linkers for click chemistry conjugation.

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Vaccine Adjuvants/VacciGrade™ products

InvivoGen provides the largest choice of vaccine adjuvants.

They are either already approved for use in human vaccination or still under investigation. They include particulate adjuvants, such as alum and emulsions, and molecules known to activate pattern recognition receptors (PRR), referred to as PRR agonists. Adjuvants are essential for enhancing and directing the adaptive immune response to vaccine antigens. They not only potentiate the immunogenicity and protection efficacy of vaccines, but they also help to avoid an exacerbated TH2-polarized response.

Adjuvants are known to exert their effect through different mechanisms. These include:

  • Alum & Emulsions – generate depots that trap antigens at the injection site for a sustained stimulation of the immune system through an increased recruitment and activation of antigen presenting cells (APCs).
  • PRR agonists – effectively activate a local innate immune response, predominantly targeting the APCs, and consequently influencing the necessary adaptive immune responses. Members of nearly all of the PRR families are potential targets for adjuvants.

InvivoGen adjuvants are VacciGrade™, a specific grade for preclinical studies. They are prepared under strict aseptic conditions, guaranteed sterile, and are thoroughly tested for the presence of endotoxins. InvivoGen also offers the standard antigen ovalbumin

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Cell Lines

InvivoGen provides an expanding collection of human and mouse cell lines in which specific genes are over-expressed or knocked out.

InvivoGen's cell lines are reliable tools for:

  • studying specific gene function(s)
  • investigating cell signaling pathways and signal transduction
  • screening agonists/antagonists of pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs), C-type lectin receptors (CLRs), cytosolic DNA sensors (CDSs), and aryl hydrocarbon receptor (AhR)
  • measuring cytokine production
  • validating antibody function

Most of InvivoGen's cell lines are engineered to stably express an inducible secreted reporter gene. We offer two reporter systems relying either on the secreted embryonic alkaline phosphatase (SEAP) or the Lucia luciferase. The activity of these two reporter proteins is readily measurable in the cell culture supernatant using InvivoGen's detection reagents. Their cell lines are extensively tested for viability, biological activity and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, thus minimizing the need for optimization or troubleshooting by the end-user.

Reporter Cells Product Flyer:

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Antibodies & ELISAs

InvivoGen provides:

  • collection of monoclonal (mAbs) and polyclonal (pAbs) antibodies that target pattern recognition receptors (e. g. anti-TLRs, anti-NLRs) and cytokines. They can be used for neutralization, flow cytometry and/or Western blot applications.
  • Well-known monoclonal antibodies (e. g. anti-TNF, anti-CD20, anti-PD1) with the most common human and murine isotypes.
  • Plasmids for antibody generation that facilitate isotype switching and efficient purification of antibodies from biological samples.
  • Different purification supports including Peptide MProtein L, and Protein G.
  • Bioluminescent ELISA kits (LumiKine™ Xpress kits) designed to quantify the levels of cytokines in cell culture supernatant, serum and plasma samples.

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InvivoGen offers a collection of inhibitors suited for the study of innate immunity signaling pathways, including modulators of various actors implicated in pattern-recognition receptor (PRR) and inflammasome signaling, as well as autophagy pathways. Additionally, we provide a set of small molecule inhibitors acting at different stages of the SARS-CoV-2 infection to support COVID-19 research, and prodrugs acting as DNA synthesis inhibitors to achieve cell selection using suicide genes. InvivoGen's collection of inhibitors is for research only use.

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Cloning & Expression Vectors

InvivoGen provides a broad range of cloning and expression vectors for different applications:

  • pFUSE-CLIg and pFUSE-CHIg plasmids for antibody generation and isotype switching
  • pBROAD and pWHERE are optimized vectors for rodent transgenesis
  • expression plasmids, such as pVITRO and pVIVO for mammalian gene expression in vitro and in vivo, pSELECT-Tag for the expression of tagged genes, pDUO for the simultaneous expression of two genes
  • for DNA vaccination, pBOOST can enhance immunogenicity or induce a Th1/Th2 biased response, while pVAC facilitates the induction of neutralizing antibodies.

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Genes & Promoters

InvivoGen provides an expanding collection of human and murine genes cloned in an expression plasmid.

All the genes are provided as open reading frames (ORFs) from the ATG to the Stop codon and are fully sequenced. They are flanked by unique restriction sites to facilitate their subcloning into another vector. A wide selection of cellular promoters of human and rodent origins is available in the expression plasmid pDRIVE. These promoters are either native and consist of a single fragment from the 5' region of a given gene, or composite and results from the assembly of different promoter elements. Furthermore, the promoters direct ubiquitous or specific expression.

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