Announcing the Global Launch of DLS: New Direct Labeling Chemistry for Genome Mapping and Structural Variation Analysis

launch-of-dls.jpgWe are pleased to announce the global commercial launch of new chemistry for sequence motif labeling called Direct Label and Stain (DLS). DLS is a non-destructive labeling chemistry that improves every aspect of Bionano genome mapping. When used in the Bionano workflow, DLS enables unprecedented sensitivity and resolution for structural variant discoveries and the most accurate and contiguous genome assemblies.

DLS kits launch as a companion to our existing NLRS (nick, label, repair and stain) labeling kits. Unlike NLSR, which introduces sequence-specific nicks and incorporates labeled nucleotides as part of the DNA repair step, DLS directly applies labels to the DNA using sequence-specific motifs without damaging the DNA. By removing the destructive steps in the workflow, the systematic molecule breaks seen using NLRS are eliminated. The DLS protocol streamlines the sample prep process. It involves fewer steps, uses fewer enzymes and a single sequence motif map is usually sufficient to obtain the same or better results seen with the NLRS chemistry, effectively doubling the throughput of the Saphyr system.

bionano-logo.jpgThe DLS chemistry has been evaluated and hardened by Saphyr users in an extensive early access program, which revealed the robustness and significant additional value of the new approach. For example, genome maps generated using DLS are 50-fold longer on average than when using the NLRS chemistry. Chromosome arms and full chromosomes are often assembled in single maps, reducing the need for other chromosome-scale scaffolding methods. Sequence assemblies scaffolded with DLS maps reach the highest contiguity and accuracy reported to date. DLS also improves sensitivity for all structural variant calls, yielding robust detection of heterozygous insertions and deletions as small as 500 bp, which is well below the previous limit of 1kb. DLS also improves sensitivity to larger balanced and unbalanced structural variants, which had been impacted by the systematic strand breaks associated with high-density nickase-based labeling.

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DLS Kits are Available for Order Now!


DLS technical requirements

DLS is compatible with DNA isolated by any Bionano Prep protocol. Bionano Access™ v1.2 and Bionano Solve v3.2 are optimized to work with DLS, with typical human de novo assemblies taking less than 30 hours on a single Saphyr or Bionano Compute. DLS is not compatible with Irys® systems. Contact us for an upgrade offer if you are interested in upgrading from Irys to Saphyr.

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