On this page:
Applications
Genomics
NanoCellect's microfluidic cell sorter, the WOLF, allow the cell's integrity to be maintained for numerous sequencing and multi-omics techniques.
- 10x Genomics RNA-seq library prep
- Single-Cell sorting on PCR plate
- Whole-genome sequencing methods
Antibody Discovery
The WOLF cell sorter provides a simple, gently cell sorting solution for many workflows such as:
- Antibody Discovery from Single Bovine B-Cells
- Isolate Antibody-Secreting CHO Cells
- Recombinant protein production in CHO Cells
Cell Line Development
The WOLF's ability to protect cell viability after sorting makes it an integral part of any cell line development workflow such as:
- Recombinant protein production in CHO Cells
- Sorting stem cell cultures
- Antibody discovery
Gene Editing
Microfluidic cell sorting with the WOLF, along with single cell deposition using the N1 single cell dispenser, eliminates the challenges that pluripotent stem cells face by sorting at gentle pressures without decompression shock and shear stress, and allow for efficient single cell deposition for high monoclonal outgrowth.
- CRISPR and disease modelling
- Protection of Karyotype
- Generation of Genome-Edited iPSCs
Top of page
WOLF G2 Cell Sorter
Gentle Benchtop Microfluidic Cell Sorting
-
Compact and at your bench
- Significantly expanded the capabilities of gentle benchtop microfluidic cell sorting with two lasers and up to nine colours,
- Simple workflows for either bulk sorting or single-cell dispensing
- Single-cell sorting can be completed in 96- or 384-well plates
- Ideal for use in many different research fields and application areas like single-cell genomics, cell line development, gene editing, antibody discovery, immunology, infectious disease, basic research, and more.
Top of page
WOLF Cell Sorter
Simple benchtop cell sorter
The WOLF was designed and created by a team of scientists and engineers who wanted to solve a classic challenge in biological research: how to sort cells of high quality effectively and easily.
Live cells can be damaged by conventional cell sorters, resulting in a low-quality cell population. At <2 psi, the WOLF's microfluidic channel is gentler than any conventional cell sorter or flow cytometer, allowing for higher populations of healthy, sorted cells with improved cell viability.
There's no need to be a flow cytometry expert in order to use the WOLF. The software interface is intuitive, user-friendly and simple. There is no fluidics cart, and the optics are fixed – resulting in less complexity, less clean-up, and more time for experiments.
The WOLF allows for maximum flexibility, accessibility and convenience as a benchtop cell sorter in individual labs. At 2 cubic feet, the WOLF has a small footprint and easily fits inside a biosafety cabinet.
The WOLF doesn't need a fluidics cart and uses a disposable cartridges so maintenance is simple and economical. Save money on important experiments by using an affordable and approachable cell sorter.
The WOLF utilizes a sterile and disposable cartridge, which makes sorting biohazardous samples safe by not producing any aerosols. Cross-contamination is never an issue, as every cartridge is disposable and allows for a clean sample run, every time.
Top of page
N1 Single-Cell Dispenser Accessory
A module for the WOLF Cell Sorter to sort and dispense into 96-well or 384-well plates.
The N1 single-cell dispenser is ideal for a multitude of applications including single cell cloning and works seamlessly with the microfluidic-based WOLF cell sorter for easy single cell isolation, single cell analysis, and cell line development. The gentle low-pressure sorting mechanism and single cell manipulation preserve cell viability through the sorting process and provide higher outgrowth of healthy clones. Reduced stress via gentle microfluidic cell sorting (low throughput) process also avoids potential adverse gene expression or karyotype changes experienced with a traditional cell sorter.
The WOLF provides 5 parameters for analyzing and sorting cells. When combined with the N1 Single-Cell Dispenser accessory, it allows higher rates in single cell detection in wells compared to cell printers or limited dilution methods. Deposition above 90-95% are routinely observed.
Top of page
Microfluidic Cartridges
Better than a fluidic cart, sterile microfluidic cartridges ensure low-pressure cell sorting applications for sensitive cells.
With disposable cartridges, there will be no more sample-to-sample contamination
WOLF Calibration Beads
The WOLF Cell sorter requires to use calibration beads to complete the initialization of the microfluidic sorting cartridge. NanoCellect provides calibration beads in convenient dropper bottle. Just drop 1 mL
Top of page
Better than a fluidic cart, sterile microfluidic cartridges ensure low-pressure cell sorting applications for sensitive cells.
The WOLF Cell sorter requires to use calibration beads to complete the initialization of the microfluidic sorting cartridge. NanoCellect provides calibration beads in convenient dropper bottle. Just drop 1 mL
