10x Genomics - Monthly Newsletter (August)

10x_logo_vertical_full-color_digital.pngWelcome to our monthly 10x Genomics update!


Contents:




Events

Virtual Conference Series

10x Genomics and Millennium Science are proud to support the OzSingleCell Webinar Series.

Episode 3: Adding Spatial Context to Single-Cell Sequencing
Date: Tuesday 1st September, 2020
Time: 8:30am AEST

Register Now


To see all events in the Webinar series, or to access recordings of past episodes click to view the OzSingleCell website below.

Visit Oz Single Cell Website



Virtual Symposium

The University of Melbourne Webinar: Single Cell Genomics in Biomedical Science.

A mix of international, national, and local speakers will discuss the latest discoveries, the practicalities and the challenges of applying single-cell genomics approaches in the biomedical sciences.
Hosted by School of Biomedical Sciences Research Committee, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne.

Date: Tuesday 8th September, 2020. Half day event
Time: 9:30am AEST

Register Now




New Product

Single Cell Multiome - ATAC and Gene Expression

Simultaneously profile gene expression and open chromatin from the same cell with Chromium Single Cell Multiome ATAC + Gene Expression. Multiply your power of discovery to characterize cell types and states, and uncover gene regulatory programs! Check the resources here:




Other Product Updates

Immune Profiling v2 Update

With the latest improvements, Single Cell Immune Profiling v2 vastly increases sensitivity, enabling detection of rare cell populations and phenotypes. Comprehensive immunophenotyping with this scale and resolution has never been more accessible.

Check out the Product Page



Product Discontinuation Notification

The final order date for Single Cell CNV reagent kits is Dec 31, 2020. Here is a product information sheet. Please note that we no longer sell Single Cell CNV Chips in the APAC region.




Resources

New Immunology Infographics




iPSC Sample Prep for 10x with NanoCellect

Gently Sort iPSCs upstream of 10x Genomics applications.

Induced Pluripotent Stem Cells (iPSCs) have made a dramatic impact in biological research where they are used to study tissue and organ development, drug discovery, and a wide range of diseases. Single-cell RNA sequencing, has become a valuable tool to study the diversity and heterogeneity of cells contributing to a phenotype. Since iPSCs are fragile cells, mechanical stress can obscure or skew the scRNA-seq results by activating genes related to cell stress or differentiation.

This application note demonstrates that using a cell sorter upstream of your 10x Genomics applications results in improved library complexity.




Frequently Asked Questions

paul.jpg Hello and welcome to the 10x Newsletter.
My name is Paul Gooding and I'm the Field Application Scientist with Millennium Science for their Genomics portfolios in Australia and New Zealand.
I thought it would be fun to host an FAQ section where I could post the most common questions that I get asked about 10x Genomics solutions and technologies, and the most up to date responses. There is also a link to send in your own question, I will do my best to answer it in the following newsletter


What is the best way to count cells?

Accurate cell counting is critical for success with single cell experiments. The following cell counting methods are recommended:

  • Countess II Automated Cell Counter (Thermo Fisher), cell size range 7-60 µm, limited in measuring viability of very small cells
  • Hemocytometer, any cell size range
  • Scepter Handheld Automated Cell Counter (Millipore), cell size range 3-18 µm and 8-25 µm, limited dynamic range

The aim is to generate a single cell suspension between 700 - 1200 cells/µl. The cell suspension should be counted at least 3-4 times and the average of all measurements should be taken. The standard deviation between all counts should not be >25%.

A flowchart outlining the recommended sample preparation procedure can be found here

There is also a related Technical Note here

Have a question for Paul? Submit your question here




Over 1,200 Publications with 10x Technology!

It was only recently that 10x Genomics announced that they hit 1,000+ publications, but they are now over 1,200! 

10x Genomics have a publication search tool on their website, where you can filter by market segment, species, tissue type, journal, and 10x product. Click the button below to explore what they can do with 10x technology.


Search the Publication Database


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