PacBio - Monthly Newsletter (September)

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Welcome to the PacBio News and Events Update!


Contents:




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Sequence all life with confidence.
With PacBio HiFi reads, you no longer need to compromise between long read lengths and high accuracy sequencing.


Enter to win PacBio highly accurate long-read sequencing.

Simply tell us in 300 words or less how HiFi reads will accelerate your science for a chance to win up to 4 SMRT Cells 8M, sequencing on the new Sequel II System and bioinformatics support from the joint AGRF-UQ PacBio Service team

The submission window closes October 12, 2020.


Submit Proposal


Please email us if you should have any questions on this SMRT Grant Program.
We look forward to learning how HiFi sequencing will benefit your research.

Warm Regards,
The PacBio APAC & Millennium Science Teams


Thank you to our sponsors; AGRF and UQ for supporting the PacBio ANZ HiFi SMRT Grant!

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PacBio Virtual Library Prep Workshop

Date: Thursday 13th August, 2020
Time: 12:00pm AEST


Are you planning to do PacBio sequencing?
Would you like to be able to make your own libraries?

Join us for this fun half day workshop to learn how easy it is to make your own PacBio libraries that can be sequenced at a PacBio service provider.

Learn what equipment you might need for different library types and how this might be easier than you realised.


Register Now


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Building Better Genomic Resources


Webinar Event: PacBio - Beyond a Single Reference Genome: The Advantages of Sequencing Multiple Individuals

Date: Thursday 17th September, 2020
Time: 3:00am AEST

Hear how scientists are using PacBio highly accurate long reads (HiFi reads) to develop pangenome collections and to study population genetics of plant and animal species to power their research. Learn about the advantages of sequencing multiple individuals to gain comprehensive views of genetic variation and understand the speed, cost, and accuracy benefits of using HiFi reads.

Speakers:
Kristin Mars, Sequencing Application Sales Specialist, PacBio
Matthias H. Weissensteiner, Ph.D. Postdoctoral Researcher, Penn State University
Kevin Fengler, Computational Genomics Lead, Data Science and Informatics, Corteva Agriscience.


Register Now



Virtual Conference Event: T2T/HPRC Towards a Complete Reference of Human Genome Diversity

Date: Tuesday 22nd - Wednesday 23rd September, 2020

The Telomere-to-Telomere (T2T) consortium is an open, community-based effort to generate the first complete assembly of a human genome.

This is event is hosted by the T2T/Human Pangenome Reference Consortium to discuss the latest progress on joint consortia effort in terms of human WGS. PacBio HiFi will feature in many of the presentations of this important event.

Register now to attend and get the recordings after the event.


Register Now




Application Spotlight: Rare Disease

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PacBio HiFi sequencing provides comprehensive detection of all variants in a genome. Increased variant detection improves power to link genetics to phenotypes of interests for novel discovery of genes and causative variants, including for resolving genetic component of rare disease

New Answers in Rare Disease with Long-Read Sequencing provides a great overview of benefits of PacBio sequencing for solving Rare and Mendelian Disease. Plus check-out an infographic.

More resources on Rare disease:




Plant Science News

Marker Assisted breeding case study:

  • A Rosaceae Family-Level Approach To Identify Loci Influencing Soluble Solids Content in Blackberry for DNA-Informed Breeding
  • Read about how researchers studying blackberry have used PacBio HiFi for identifying SNP markers for marker assisted breeding program in a plant that has scarce genome information.
  • "...the long reads generated by PacBio sequencing enabled identification of individual haplotypes within each of the sequenced samples. Considering the variable ploidy and the complex genetics found in cultivated blackberry, the haplotypes present at a locus can be difficult to identify and define with short-read sequencing. To reduce consensus sequence complexity and increase primer design success, identification and preference of individual haplotypes that contained the target allele was important for diagnostic marker development."

A TAL Tale: PacBio Sequencing Helps Unravel Mechanisms of Plant Infection

  • Assembling the highly variable, repetitive TAL effector gene regions was a long-standing obstacle for plant pathologists. The key to finally unraveling the tangled assemblies has been found with PacBio long-read sequencing.  Read the blog about this here



Target Capture: HLS-CATCH

sagehls_918.pngWhile targeted capture has been used in genomic studies for years, it has traditionally been possible only for short stretches of DNA. Capturing longer genomic regions or entire genes has been a major scientific need.

The Sage Science team launched the HLS-CATCH method to address this need. By pairing the SageHLS instrument for automated extraction or purification of extremely long DNA fragments with CATCH, a CRISPR-based technique for snipping out regions of interest with Cas9 guide RNAs, the HLS-CATCH method allows scientists to capture large, complex regions for cost-effective analysis.



Subscribe to the PacBio Monthly Newsletter by Millennium Science here



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