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All Sequel Systems support the full range of SMRT Sequencing applications for generating genomes, transcriptomes, and epigenomes.
For humans, plants, animals and microbes including de novo sequencing and variant detection
Study relevant genome targets across any regions of interest
In-depth analysis of cDNA sequences across the entire transcriptome or targeted genes
Learn more about Single cell RNA Sequencing with HIFI Reads
Understand variants among bacterial, viral and cancer cell populations
Detect DNA modifications in your samples while you sequence on the PacBio platform
The PacBio Sequel II and IIe Systems provide all scientists with access to high throughput, cost effective, highly accurate long-read sequencing.
Watch this short video to learn how the Sequel II and IIe Systems can help you be confident in your discoveries made possible with comprehensive views of genomes, transcriptomes, and epigenomes.
The Sequel System Family of Instruments
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Sequel IIe
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Sequel II
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Sequel
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Supported SMRT Cell
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SMRT Cell 8M |
SMRT Cell 8M |
SMRT Cell 1M |
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Number of HiFi Reads >99%* Accuracy
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Up to 4,000,000 |
Up to 4,000,000 |
Up to 500,000 |
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Sequencing Run Time per SMRT Cell
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Up to 30 hrs |
Up to 30 hrs |
Up to 20 hrs |
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Recommended Chemistry
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2.0 |
2.0 |
3.0 |
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Instrument Control Software
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v10.1 |
v10.1 |
v8.0 |
SMRT Link
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v10.1 |
v10.1 |
v10.1 |
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Performance Data
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Sequel IIe
System Release
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Sequel II
System Release
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Sequel
System Release
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Features of all PacBio Sequel Systems
Setting a New Standard in Long-Read Sequencing
The Sequel II System has been recognized for its ability to generate longer reads with greater accuracy and throughput, at a significantly lower cost.
Get to know the Sequel II and IIe Systems and their cost-effective sequencing applications with our Interactive Brochure.
Software
The Sequel Systems software package includes SMRT Link, which enables a single point of access to a suite of PacBio software tools, Sequel Instrument Control Software, and remote instrument monitoring.
Workflow
The Sequel Systems offer a simple workflow with rapid time to result.
We have introduced novel technologies at every stage of the sequencing process — from preparation to data analysis — to ensure an efficient workflow that delivers exceptional, precise results. Template preparation to primary base-call analysis takes less than a day, with typically fewer than four hours of hands-on time.
The award-winning PacBio Sequel Systems are powered by Single Molecule, Real-Time (SMRT) Sequencing technology and deliver highly accurate long reads.
Learn how to go from DNA to discovery with SMRT Sequencing in this short video.
Original Publication: Eid, J., et al. (2009) Real-time DNA sequencing from single polymerase molecules. Science, 323(5910), 133–138.
- Long Reads - With reads tens of kilobases in length you can readily assemble complete genomes and sequence full-length transcripts.
- High Accuracy - Sequencing free of systematic error achieves >99.999% consensus accuracy. Explore the benefits of highly accurate long-read sequencing.
- Uniform Coverage - No bias based on GC content means you can sequence through regions inaccessible to other technologies.
- Single-Molecule Resolution - Capturing sequence data from native DNA or RNA molecules enables highly accurate long reads with >99.9% single-molecule accuracy.
- Epigenetics - With no PCR amplification step, base modifications are directly detected during sequencing.
HiFi Reads for Highly Accurate Long-Read Sequencing
With HiFi Sequencing you get the benefits of short reads and traditional long reads in one easy-to-use technology.
Watch this short video to learn how HiFi Sequencing is empowering scientists to strive for new breakthroughs.
Advanced scientific discoveries require sequencing data that is both accurate and complete. Single Molecule, Real-Time (SMRT) Sequencing technology has evolved to a different type of long read, known as highly accurate long reads, or HiFi reads.
How Does HiFi Sequencing Compare with Other Technologies?
HiFi reads let you accurately detect all types of variants, from single nucleotide to structural variants, with high precision and recall and phase haplotypes, even in hard-to-sequence regions of the genome. Explore the resources below for more information on how HiFi reads perform relative to other technologies.
Original Publication: Wenger, A. M., et al. (2019) Accurate Circular Consensus Long-read Sequencing Improves Variant Detection and Assembly of a Human Genome. Nature Biotechnology, 37, 1155–1162.
Pacbio provide bundled reagent kits by applications to easily kick-start your long-read sequencing projects with the Sequel II System.
Quickly create libraries for Single Molecule, Real-Time (SMRT) Sequencing with our template preparation kits starting from gDNA, cDNA, or amplicons. Our single-tube, addition-only strategy minimizes hands on-time to generate libraries in as little as 4 hours.
SMRTbell library and barcoding consumables for optional multiplexing on the PacBio Sequel Systems.
Sequence multiple samples on the same SMRT Cell to increase throughput and enhance the efficiency of your workflows. Multiplexing is supported with three barcoding options providing flexibility to incorporate unique sample identifiers during target amplification or library preparation.
Learn more about Multiplexing, including information on barcode sequences compatible with SMRT Sequencing
DNA polymerase binding kits contain sequencing polymerase and reagents to prepare SMRTbell libraries for long-read sequencing on the PacBio Sequel Systems.
SMRT Cells, Sequencing Kits, and accessories support automated SMRT Sequencing on the PacBio Sequel Systems. Each system has specific SMRT Cells and Sequencing Kits to analyse a wide spectrum of different sample types.
ANZ Certified Service Providers (CSP)
The PacBio Certified Service Provider Program is a global network of validated sequencing service labs that provides access to Single Molecule, Real-Time (SMRT) Sequencing.
AGRF-UQ PacBio Service
Phone: +61-3-9321-3700
Email: PacBio-UQ@agrf.org.au
Instrument(s): Sequel II System
PacBio: Lunch & Learn Series - Season 2 (Spring 2021)
PacBio: Lunch & Learn Series - Season 1 (Mar 2021)
PacBio Doherty Webinar Series
PacBio Library Prep Workshop - Held on 24/9/20
With HiFi Sequencing you get the benefits of short reads and traditional long reads in one easy-to-use technology.
