Recombinant Proteins

Recombinant proteins are proteins encoded by a gene - recombinant DNA - that has previously been cloned in a system that supports the gene expression and translation of messenger RNA.

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Human Cell-expressed Proteins

Recombinant proteins are proteins encoded by a gene - recombinant DNA - that has previously been cloned in a system that supports the gene expression and translation of messenger RNA.

Proteins coexpressed in bacteria will not possess post-translational modifications, e.g., phosphorylation or glycosylation. For activity, many proteins require glycosylation and processing only available in eukaryotic systems. Human stem cell technology and CAR-T cell therapy also require culture media whose components must be animal component and xenobiotic free.

Humankine recombinant proteins are created in HEK293 cells using animal-free components. Our human expression system ensures that proteins have native conformation and post-translational modifications to optimize biological activity. No expression tags, xeno-free…just high quality proteins.

All Humankine recombinant proteins are:

  1. Animal-free and tag-free
  2. Made from human HEK293 cells
  3. Native post-translational modifications and folding
  4. Can be used for culturing human stem cells for regenerative medicine

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Cytokines & Growth Factors

Cytokines are small secreted proteins. Cytokines are a category of signaling molecules that mediate and regulate immunity, inflammation, and hematopoiesis. They exist in broad families that are structurally related but exhibit diverse functions (e.g., in the TNF/TNF receptor superfamily, lymphokines are also known as interleukins (ILs)).

Growth factors promote cellular differentiation and proliferation. They act on target tissues and regulate processes including tissue morphogenesis, angiogenesis, cell differentiation, and neurite outgrowth. They are essential growth-regulatory molecules for cell culture maintenance. Non-transformed cells show an absolute requirement for growth factors for maintenance in cell culture and for most cell types, more than one type of growth factor is required. Under cell culture conditions, growth factors are the most rapidly depleted media components and thus become a rate-limiting factor for further proliferation. Commonly used growth factors in tissue engineering include fibroblast growth factors (FGFs), vascular epithelial growth factors (VEGFs), and transforming growth factors-β (TGF-βs). The loss or decreased level of growth factors is common in neoplastically transformed cells and may lead to an uncontrolled growth of cancer cells.

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E.coli-expressed Fusion Proteins

Fusion proteins are useful tools for many laboratory techniques, such as protein purification or blocking experiments. Proteintech offers a wide range of high-quality fusion proteins. Our customer service provides fusion protein and peptide libraries, that can be used as a standard and a control in cell assay and immunoassay (Please note our fusion proteins are not tested for the activity and are not suitable for in vivo research).

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