Single Cell Transcriptomics

Gene Expression


single-cell-solution-with-monitor.pngGo Beyond Traditional Gene Expression Analysis

The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for cell characterization and gene expression profiling of hundreds to tens of thousands of cells. Our latest improvements allow you to detect even more unique transcripts per cell and with the addition of Feature Barcoding technology, you can get a more complete molecular readout cell by cell—identify cell-specific CRISPR-mediated perturbations or simultaneously measure gene and cell surface protein expression in the same cell, with virtually unlimited possibilities for the additional feature types.

Go beyond traditional gene expression analysis to characterize cell populations, cell types, cell states, and more on a cell-by-cell basis. From assessing tumor heterogeneity and stem cell composition, to dissecting neuronal populations—the technological advancements provided by the Chromium Single Cell Gene Expression Solution, along with turnkey software tools, allow the creation of high complexity libraries from single cells to maximize insight from any sample type.


  • Identify and characterise rare cell types.
  • Analyse and understand cellular heterogeneity and how this contributes to your biological system.
  • Perform cellular phenotyping with single cell RNA-seq to identify novel targets, biomarkers, and cell types and states without the need for pre-selected targets.
  • Evaluate mRNA and cell surface protein expression profiles within the same cell.
  • Perform high throughput and high resolution functional genetic screens in tens of thousands of cells simultaneously.
  • Assess comprehensive gene expression phenotypes for individual CRISPR perturbations.



Benefits:

  • Partition 500 - 80,000+ cells in under 9 minutes
  • Superior sensitivity and cell capture rate up to 65%
  • Simple workflow
  • Cell size flexibility, no lower limits
  • Low doublet rates of under 0.9% in 1,000 cells



Workflow:

single-cell-workflow.png



Gene Expression Profiling

Gene Expression CRISPR Screening

Gene and Cell Surface Protein




Immune Profiling

single-cell-vdj-solution-with-monitor.pngHuman or Mouse Immune Cell and Repertoire Profiling at Single Cell Resolution

The Chromium Single Cell Immune Profiling Solution is a comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells in human or mouse on a cell-by-cell basis. With the addition of Feature Barcoding technology, you can also detect and analyze additional cellular readouts, such as cell surface markers and antigen specificity, to enhance immune cell phenotyping and study dynamic interactions between lymphocytes and target cells.

With our streamlined workflows, you can go from sample to library prep, immune sequencing, and software analysis, revealing insights into T and B cell diversity, V(D)J recombination, and immune cell profiling. From immunology and immuno-oncology research, to infectious disease research and beyond – these solutions will accelerate the understanding of the adaptive immune system.


  • Reveal clonality, diversity, antigen specificity, and cellular context
  • Assemble and annotate full-length V(D)J gene sequences
  • Pair α and β chain TCR sequences from individual T cells
  • Pair heavy and light chain immunoglobulin (Ig) sequences from individual B cells with full isotype resolution
  • Simultaneously measure TCR, B cell Ig, cell surface protein expression, and 5' gene expression in the same cells
  • Link full-length, paired TCR α and β chain sequences with TCR-pMHC specificity
  • Simultaneously measure cell surface protein expression with gene expression



Benefits:

  • Rapidly partition 100 - 80,000+ cells
  • Superior sensitivity
  • Simple workflow
  • Cell size flexibility, no lower limits
  • Highest cell capture rates of up to 65%
  • Low doublet rates of under 0.9% in 1,000 cells



Workflow:

single-cell-vdj-workflow.png



Immune Profiling

Immune Profiling and Cell Surface Protein

Immune Profiling and Antigen Specificity



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