Systems

Product List




BluePippin

bluepippin-products.jpg DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field

The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination.


Benefits

  • Saves time and effort compared to manual gel purification.
  • Sample-to-sample reproducibility streamlines research.
  • Higher sample quality improves downstream analysis.

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PippinHT

pippin-ht-with-computer-2250.png High Throughput Size Selection for Next-Gen Sequencing

PippinHT incorporates the company's highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing large-scale sequencing data.


Benefits

  • Sample-to-sample reproducibility streamlines research.
  • Higher sample quality improves downstream analysis.
  • 24 sample capacity meets high throughput sequencing demands.

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Pippin Prep

pippin-prep-hero.png DNA Size Selection for Next-Gen Sequencing

The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination.


Benefits

  • Saves time and effort compared to manual gel purification.
  • Sample-to-sample reproducibility streamlines research.
  • Higher sample quality improves downstream analysis.

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Pippin Pulse

pippin-pulse-hero.png Pulsed-field Power for Gel Boxes

Sage Science is pleased to introduce the new Pippin Pulse electrophoresis power supply for analytical gels. They've taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond. The Pippin Pulse features a simple software interface to provide users full flexibility to develop their own field-inversion protocols. The operating range is 25-175V, and up to 200 mA. The Pippin Pulse includes the power supply, cables, software (for PC) for programming, and user manual.

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SageELF

elf_casing_hero.png Whole Sample Fractionation of DNA or Protein

The SageELF is an electrophoresis system that separates DNA or protein samples by size, and then fractionates the whole sample, or section of sample, into 12 fractions. The system is equipped with pulsed-field electrophoresis for resolving large DNA.

Fractionation ranges are estimated and adjusted in software, and fractions are collected in buffer. One sample is fractionated on a single precast agarose cassette, and one or two cassettes may be processed at one time.


Benefits

  • Reduces the complexity of RNA (cDNA) or protein samples.
  • Allows construction of DNA libraries with multiple insert sizes for improved assembly.
  • Allows sequencing of valuable DNA and retrieving sample for later study.

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SageHLS HMW Library System

hls-open-final-500px-e1486946320337.png Extract. React. Select.

The SageHLS system provides a new approach to DNA extraction. Users load cells suspensions on Sage Science gel cassettes to perform lysis under electrophoretic conditions. This removes degraded and solubilized proteins but leaves intact DNA behind, bound in agarose. A cleavase is then used to carfully fragment the DNA into electrophoretically-mobile sizes. Automated DNA size selection collects the DNA in 6 size bins.



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