Transfection

Dharmacon transfection reagents are designed specifically for small RNA transfection (DharmaFECT), co-transfection of plasmid and small RNA (DharmaFECT Duo), plasmid transfection (DharmaFECT kb) and self delivering siRNA for difficult to transfect cell types (Accell siRNA).

DharmaFECT 1 Transfection Reagent

The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments. DharmaFECT 1 has been validated in over 35 cell types.

DharmaFECT 2 Transfection Reagent

One of four siRNA/microRNA specific formulations, DharmaFECT 2 is a chemically distinct alternative to one-size-fits-all transfection reagents to achieve high-efficiency silencing in more cell types.

DharmaFECT 3 Transfection Reagent

One of four siRNA/microRNA specific formulations, DharmaFECT 3 is a chemically distinct alternative to one-size-fits-all transfection reagents to achieve high-efficiency silencing in more cell types.

DharmaFECT 4 Transfection Reagent

DharmaFECT 4 Transfection Reagent is chemically distinct from the other DharmaFECT formulations, providing an alternative when DharmaFECT 1 does not achieve optimal results, and is ideal to include in transfection optimization experiments prior to RNAi screening or other high-value experiments.

DharmaFECT Set of 4 Transfection Reagents

An aliquot of each of the four DharmaFECT formulations for siRNA/microRNA transfection optimization studies. Useful for determination of the best reagent for your cells and particular experimental conditions.

DharmaFECT Duo Transfection Reagent

Optimized for co-transfection, DharmaFECT Duo transfection reagent provides highly effective delivery of siRNA, microRNA or crRNA reagents with plasmid. Useful for experiments utilizing reporter genes, RNAi rescue, or Edit-R Cas9 plasmid.

DharmaFECT kb Transfection Reagent

A unique transfection reagent from our DharmaFECT product line for introduction of DNA into cultured cells with high efficiency and low cytotoxicity.

Accell siRNA

Specially modified for delivery into difficult-to-transfect cells without a transfection reagent, Accell enables non-viral RNAi in neuronal, immune, and stem cells.

Products

Accell siRNA Controls

Effectively optimize Accell siRNA delivery conditions and control for variables in ongoing experiments with fluorescent or unlabeled positive and negative controls. Accell Control Kits combine species-specific validated controls for assessment of Accell technology in your difficult-to-transfect cells.

Cherry-pick RNAi Libraries

Fast and easy online configuration and ordering of plated siRNA & microRNA reagents targeting your genes of interest.

Custom siRNA Synthesis

Use the online design tools and extensive synthesis options to create a custom siRNA specific for your application. Numerous combinations of modifications, sizes, and purification options are available for convenient online ordering.

Products

Midscale and ORM RNA & DNA Synthesis

Midscale/OEM service for synthesis of RNA and DNA at larger quantities.

Order Custom siRNA

Order both modified or unmodified siRNAs. Patented modification patterns (ON-TARGET, ON-TARGETplus, siSTABLE, or Accell) and additional custom modifications are available.

Customer SMARTpool design & synthesis

To complement our pre-designed siRNA collections and support a broad range of RNAi experiments, our siRNA design experts can provide custom SMARTpool reagents targeting genes outside of our genomewide offerings

siDESGIN Center

Design siRNAs targeting genes in non-standard species, particular splice variants or homologous regions across gene families or species.

Human siRNA libraries

Human siRNA libraries are available in SMARTpool or individual siRNA format for ON-TARGETplus, siGENOME and Accell technologies. Learn more about each product line to make the right choice for your experimental needs. GE Dharmacon support more strategies for successful screening and more screening expertise than any other provider. Find the predefined gene family, including Druggable genes or Whole Genome, that's right for your discovery efforts.

Mouse siRNA Libraries

An arrayed collection of siRNA reagents for RNAi screening, targeting mouse kinases. Accell siRNA permits RNAi screening in difficult-to-transfect cells with no transfection reagent, virus, or electroporation. A wide selection of predefined siRNA libraries, including the most up-to-date Whole Mouse genome collection available.

Highlights

Accell siRNA enters cells without the need for transfection regents, virus (or viral vectors), or instruments.
Proven performance in neuronal, immunological, primary, and other difficult-to-transfect cell types.
Extended-duration knockdown with optimised continuous application.
Available as SMARTpool siRNA reagents or a Set of 4 siRNAs in 96-well plates.

Millennium Science

Enabling next-generation research

DharmaFECT 1 Transfection Reagent

DharmaFECT 2 Transfection Reagent

DharmaFECT 3 Transfection Reagent

DharmaFECT 4 Transfection Reagent

DharmaFECT Set of 4 Transfection Reagents

DharmaFECT Duo Transfection Reagent

DharmaFECT kb Transfection Reagent

Accell siRNA

Products

Accell siRNA Controls

Cherry-pick RNAi Libraries

Custom siRNA Synthesis

Products

Midscale and ORM RNA & DNA Synthesis

Order Custom siRNA

Customer SMARTpool design & synthesis

siDESGIN Center

Human siRNA libraries

Mouse siRNA Libraries

Highlights

Brands

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