Deeper Spatial Discovery with Visium HD 3′
Visium HD 3' is here! Explore more species, pre-order today
Introducing 10x Genomics‘ Visium HD 3’, a novel assay for unbiased cross-species spatial gene expression profiling of fresh frozen tissue sections mounted on a standard glass slide. The Visium HD’s array has a gapless design that enables integration of unsupervised gene expression clustering data with microscope H&E images from the same tissue section, allowing precise profiling of finer anatomical features with high tissue coverage at single cell-scale resolution.
Furthermore, this novel reverse transcription-based assay generates cDNA products compatible with both short reads and long reads sequencing, extending its applications beyond gene expression analysis to enable immune profiling and the discovery of isoforms and novel transcripts on a spatial level.
Watch Visium HD 3′ announcement at AGBT 2025.
Key Features
Visium HD 3’ assay, which delivers whole transcriptome spatial analysis with a 3’ poly(A) capture-based chemistry. This assay provides:
- Flexibility to explore the spatial biology of fresh frozen samples from any species
- De novo spatial discovery power, including isoforms, TCRs/BCRs, and SNVs
- True single cell resolution with new cell segmentation software
- Streamlined slide handling and high spatial fidelity powered by the Visium CytAssist instrument
Resources
Press Release: 10x Genomics Expands Visium Platform with Launch of Visium HD 3’ Gene Expression, Unlocking Deeper Spatial Recovery
Grant application resources for Visium products
AACR Annual Meeting (Chicago) Visium HD 3′ poster: Visium HD 3’ enables unbiased whole transcriptome spatial profiling of Tumor microenvironment in fresh frozen cancer tissues at single cell scale resolution.
Introductory webinar
Join this upcoming 10x Genomics webinar Thursday June 4th at 1:00PM AEST to explore the capabilities of the new Visium HD 3’ assay, which delivers:
- A species-agnostic option for whole transcriptome spatial analysis (see new data in chicken, zebrafish, rat, pig, and more!)
- Single cell–scale resolution powered by Visium HD slide architecture and our new cell segmentation software
- A streamlined, histology-friendly workflow with the Visium CytAssist instrument
Registration: https://10xgen.com/1Nd
