In the Lab with Dr Sabrina Sofia Burgener
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Live Cell Imaging of Inflammasome Signalling
In this webinar, Dr Burgener shares how Agilent technologies, including the Cytation 5 Cell Imaging Multimode Reader, have advanced her inflammasome research by enabling high-throughput live-cell imaging over time – providing dynamic insights into inflammasome activation and signalling outputs.
What you’ll learn:
- The role of the NLRP3 inflammasome in immune defence and sterile inflammation
- Key mechanisms: CASP1 activation, cytokine release, gasdermin D cleavage, and pyroptosis
- Limitations of single-endpoint assays in inflammasome research
- How live-cell imaging enables temporal analysis of ASC speck formation, cytokine secretion, and cell death
- Newly established, standardised assays for tracking inflammasome activation in real-time