GelRed is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed is far more sensitive than EB without requiring a destaining step. GelRed and EB have virtually the same spectra, so you can directly replace EB with GelRed without changing your existing imaging system. GelRed can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning. A series of safety tests have confirmed that GelRed is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, working strength GelRed can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. GelRed 3X in water (catalog no. 41001) is ready-to-use for post-electrophoresis gel staining, and is supplied in a 4L Cubitainer. Biotium also offers GelRed 10,000X in water (catalog no. 41003) and GelRed 10,000X in DMSO (catalog no. 41002). GelRed in water is a newer, safer formulation and our recommended format. We continue to offer GelRed in DMSO for established users who do not wish to alter their protocols. Also see GelGreen Nucleic Acid Gel Stain, a safer replacement for SYBR gel stains, which is compatible with visible blue light excitation. FEATURES
- Safer than EB: Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
- Easy disposal: Passed environmental safety tests for direct disposal down the drain or in regular trash
- Ultra-sensitive: Much more sensitive than EtBr and SYBR Safe
- Extremely stable: Available in water, stable at room temperature for long-term storage and microwavable
- Simple to use: Very simple procedures for precast or post-electrophoresis gel staining
- Compatible with a standard UV transilluminator : Replaces EtBr with no optical setting change
- Compatible with downstream applications : Gel purification, restriction digest, sequencing and cloning
