10x Genomics Products

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Chromium Single Cell

Visium Spatial

Instruments

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Fixed RNA Profiling

Fixed RNA Profiling provides single cell whole transcriptome gene expression and multiplexing capabilities to profile hundreds to a million cells. Fixation at the point of sample collection preserves fragile biology and greatly streamlines workflows to provide critical insights on more samples.

  • This product enables the comprehensive profiling of the transcriptome (human or mouse) from single cells using a probe-based approach.
  • The addition of Feature Barcode technology enables simultaneous profiling of cell surface protein expression for multidimensional insights into complex biology.
  • This assay also enables sample multiplexing to increase your throughput and workflow efficiency.

Product Sheet: Single cell profiling on more samples with no time constraints

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Single Cell Gene Expression

Profile thousands of genes at the single cell level for unbiased characterization of cell types and cell states

  • Combine gene expression analysis with detection of hundreds of cell surface proteins at high resolution for ultra-high parameter multiomic cytometry
  • Use with Targeted Gene Expression panels to focus on the genes and biomarkers most relevant to your research
  • Simultaneously assess CRISPR perturbation phenotypes and gene expression from the same cell to accelerate your functional genomics studies
  • Leverage dual index libraries for reliable sequencing data quality
  • Combine with 3' CellPlex to multiplex up to 12 samples and recover up to 17,500 singlets* per channel, enabling larger-scale single cell studies
  • Singlets are single cells or nuclei captured after multiplet removal.
  • Level up your insights by combining single cell gene expression data with spatial gene expression

Product Sheet: Explore cellular diversity, cell by cell

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Single Cell Immune Profiling

Profile thousands of genes at the single cell level by barcoding mRNA at the 5' end, for unbiased characterisation of cell types and cell states

  • Simultaneously profile immune repertoire (BCR/TCR) and gene expression from the same cell to enable correlation of clonotype with the corresponding cell subtype
  • Obtain paired, full-length receptor sequences from T cells and/or B cells with complete isotype resolution, providing functional data for antibody/TCR discovery
  • Combine gene expression analysis with detection of hundreds of cell surface proteins at high resolution for ultra-high parameter multiomic cytometry
  • Combine with Targeted Gene Expression Panels to focus on the genes that matter most and accelerate your studies into actionable insights
  • Utilise product partners for Feature Barcode technology (oligo-conjugated antibodies, oligo-conjugated MHCs) to easily perform multiomic profiling
  • Leverage dual index libraries for superior sequencing data quality

Product Sheet: Multiomic profiling of the immune system at single cell resolution

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Single Cell Multiome ATAC + Gene Expression

The first commercial single cell multiomics product for simultaneous profiling of gene expression and chromatin landscape at single cell resolution.

Capture multiple measurements simultaneously at the single cell level for deeper characterisation of cell types and states

  • Integrate gene expression with epigenomic landscape through direct measurements from the same cell, eliminating the need for computational inference across datasets
  • Link open chromatin regions and target genes to discover new gene regulatory interactions
  • Obtain multiple measurement types from the same cell to maximize insights from limited samples
  • Take advantage of a simple and robust workflow for straightforward experimental setup
  • Apply easy-to-use data analysis and visualisation software for powerful data interpretation

Product Sheet: Simultaneous profiling of the transcriptome and epigenome from the same cell

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Single Cell ATAC

Chromium Single Cell ATAC v2 is now shipping!

Chromium Single Cell Assay for Transposase Accessible Chromatin (ATAC) is a powerful method to understand gene regulation and characterise cell types and states, all at the single cell level. Building upon v1.1 chemistry, the v2 assay provides increased sensitivity and improved signal-to-noise ratio, enhancing your ability to detect rare peaks.

  • 50% higher sensitivity, depending on sample type
  • Increased usable reads, thereby improving signal-to-noise ratio
  • Deeper characterisation of complex samples, including enhanced ability to detect rare peaks
  • Compatibility with the new Chromium Nuclei Isolation Kit, an easy-to-use kit optimised for 10x Genomics single cell assays, which streamlines sample prep for a wide range of frozen sample types.

Product Sheet: Profiling chromatin accessibility at single cell resolution

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Nuclei Isolation

Prep for success: 

10x Genomics' new Chromium Nuclei Isolation Kit removes the complexities of nuclei isolation with an easy-to-use solution specifically designed for use with 10x Genomics single cell assays for gene expression and chromatin accessibility.

  • Save precious samples and remove the guesswork from nuclei prep with a unified, optimised workflow
  • Easily and efficiently prepare frozen samples with an all-in-one kit for multiple tissue types
  • Streamline your workflow and process up to 8 samples in just 1 hour

What sample types are compatible with the Chromium Nuclei Isolation Kit

Product sheet: Streamline nuclei isolation for single cell assays

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Single Cell CRISPR Screening

10x Genomics 5' CRISPR is now available for order and shipment!

5' CRISPR Product Sheet | CRISPR Infographic | User Guide & Protocols

  • Evaluate entire gene networks, key disease pathways, or specific gene functions with multiomic, single cell profiling of up to thousands of CRISPR-driven gene edits
  • Perform large-scale pathway screens to access more information per target, enabling more confident decision making for drug candidates
  • Validate hits from genome-wide association studies (GWAS) and obtain comprehensive functional information from CRISPR edits to elucidate gene function

What is the difference between the 10x Genomics Single Cell 5' and 3' CRISPR Screening assays, and which should I use?

Our 5' CRISPR screening assay is compatible with most pre-existing Cas9 guide RNA vectors. If you have an existing CRISPR library or are starting a single cell CRISPR screen for the first time, we recommend using our Single Cell 5' v2 assay which is compatible with most guide libraries with little to no optimization required.

Our 3' CRISPR screening assay requires that a specific Capture Sequence be inserted into the vector at the appropriate location for capture of the guide. If you want to compare your single cell CRISPR experiment to data from previous experiments using 3' single cell gene expression with Feature Barcode technology, we recommend continuing to use our Single Cell 3' v3.1 assay to minimize batch effects.

A clear path to gene function with single cell CRISPR screening

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Spatial Gene Expression (Visium)

Visualise gene expression within the tissue context.  Available for FFPE and Fresh Frozen samples.

  • Integrate easily with your current laboratory methods and tools for tissue analysis, letting you start your Spatial Gene Expression experiments immediately
  • Avoid the need for dissociating your samples before conducting gene expression studies to retain spatial context
  • Broaden your reach beyond predefined regions of interest to analyse the entire tissue section at once so you don't miss out on unanticipated results
  • Combine with immunofluorescence staining and imaging to gain multiomic characterisation in the spatial context
  • Combine with Targeted Gene Expression Panels to focus on the genes that matter most and accelerate your studies into actionable insights
  • Experience unparalleled insights—full tissue coverage, high resolution, increased sensitivity, and greater depth
  • Visium Cytassist instrument - coming soon!

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Visium Spatial Proteogenomics

  • Combine whole transcriptome spatial analysis with immunofluorescence protein detection in the same tissue section.
  • Gain a deeper, more holistic understanding of tissue organisation with colocalized protein detection and gene expression analysis.

  • Go back in time—Apply new biomarkers to archived FFPE samples and uncover hidden biology from the past
  • Multiomic analysis—Characterise heterogeneity more completely with spatial multomic analysis of protein and mRNA to a single tissue section
  • Powerful discovery—Envision the spatial organization of newly discovered cell types, states, and biomarkers with protein detection and whole transcriptome analysis.

Gain a new perspective on tissue complexity with simultaneous gene and protein spatial profiling

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Instruments

Whether you're just starting out with single cell, expanding your capabilities, or seeking an automated way to partition individual cells and generate barcoded, sequencing-ready libraries, you're covered. 10x Genomics' proven Next GEM technology provides a trusted path to advance your understanding of cell diversity and function.

Chromium X Series

The most flexible way to single cell.

Expand your single cell capabilities with the most advanced hardware and highest throughput flexibility, enabling multiomic analysis of hundreds to millions of cells.

Chromium Controller

Perform multiomic analysis of hundreds to tens of thousands of single cells with this compact system.

Assay compatibility:

  • Single Cell Gene Expression LT
  • Single Cell Gene Expression
  • Single Cell Immune Profiling
  • Single Cell ATAC
  • Single Cell Multiome ATAC + Gene Expression

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Chromium Connect

Combine single cell partitioning, barcoding, and/or library construction with standardised, automated workflows to maximize your productivity.

  • Obtain consistent and reproducible single cell gene expression libraries
  • Automate your single cell library preparation workflow with maximum walk-away convenience
  • Single cell suspensions to final sequencing-ready libraries
  • Parallel processing between 1 to 8 samples per run

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Visium Cytassist 

Seamlessly transition between standard histology workflows and analyte capture onto Visium slides for in-depth expression analysis.

With Visium CytAssist you can:

  • Study FFPE specimens archived in blocks or on slides
  • Expand ongoing studies of fresh frozen samples
  • Preview tissue section integrity, morphology, and staining patterns
  • Use your slides, annotate your images, choose the best sections
  • Select from powerful spatial expression tools for whole transcriptome, targeted, proteomic, or multiomic studies
  • Quickly and easily capture sample analytes for spatial library creation with Visium Spatial Solutions

Product Sheet: Bridging histology and genomics

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Xenium In Situ

Build spatial transcriptome maps with Xenium In Situ

The Xenium workflow starts with sectioning tissues onto a microscope slide. The sections are then treated to access the RNA for labeling with circularizable DNA probes. Ligation of the probes then generates a circular DNA probe which is enzymatically amplified and bound with fluorescent oligos, creating bright, easy to image signal that has a high signal-to-noise ratio. The slide is then placed in the Xenium In Situ Analyzer where the sample then undergoes successive rounds of fluorescent probe hybridization, imaging, and removal. An optical signature specific to each gene is generated, enabling identification of the target gene. Finally, a spatial map of the transcripts is built across the entire tissue section.

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