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PippinHT DNA Size Selection System

High Throughput Size Selection for Next-Gen Sequencing

Up to 24 samples/run, Select 100bp-1.5kb, Pass up to 40kb

PippinHT incorporates the company's highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin.

The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing large-scale sequencing data.

  • Benefits of the PippinHT System
  • Sample-to-sample reproducibility streamlines research
  • Higher sample quality improves downstream analysis
  • 24 sample capacity meets high throughput sequencing demands

Key Downstream Technologies: Whole genome sequencing & miRNA library isolation

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BluePippin DNA Size Selection System

DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field (100bp – 50kb)

Size-Select DNA Fragments up to 50kb

Collect HMW DNA with High-Pass Filtering

The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA.  For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user.

Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination.

Benefits of the BluePippin System:

  • Saves time and effort compared to manual gel purification
  • Sample-to-sample reproducibility streamlines research
  • Higher sample quality improves downstream analysis

Key Downstream Technologies: Mate-pair sequencing, Single molecule sequencing, Bar-coded linked reads

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Pippin Prep DNA Size Selection System

DNA Size Selection for Next-Gen Sequencing (100bp – 1.5kb)

Short Read NGS Library Construction – For Inserts from 100bp to 1.5kb

The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination. Benefits of the Pippin Prep System:

  • Saves time and effort compared to manual gel purification
  • Sample-to-sample reproducibility streamlines research
  • Higher sample quality improves downstream analysis

Key Downstream Technologies: Paired-end sequencing, emPCR, RNA-seq, ChIP-seq

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Sage Science's DNA Size Selection Technology

Pippin's disposable gel cassettes are precast with agarose. DNA sizing is determined by the detection of markers in a dedicated lane or internally in the sample lane and using those to select and collect the desired fragments based on user-input values.

Each sample lane is physically separate to eliminate the possibility of sample cross-contamination and features a branched configuration with three electrodes. DNA is separated along a gel column until the programmed fragment range reaches the branch point. The instrument then switches the active electrode to divert DNA into a membrane-bound buffer chamber. When the size range has been collected, the active electrode is switched back to the separation channel. The desired sample can be removed with a standard pipette.

DNA sizing is available with the Pippin Prep (for fragments from 100 bp to 1.5 Kb) and with the BluePippin (a pulsed-field version for fragments from 100 bp to 50 Kb).

Watch the video below to learn about DNA size selection with Pippin:

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SageELF Whole Sample Fractionation System

Whole Sample Fractionation of DNA or Protein

The fully automated SageELF (for Electrophoretic Lateral Fractionator) can be used to select DNA or proteins. It features a cassette system designed to fractionate a whole protein or DNA sample simultaneously into 12 contiguous size fractions.

 

For proteomics users, SageELF delivers 12 size fractions in SDS buffer ready for analysis or identification on a mass spectrometer.

For scientists working with DNA, the generation of 12 contiguous fractions maximizes the utility of precious samples and can be used to build multiple libraries of different insert sizes for both short-read sequencers and long-range technologies that can assess structural information.

Benefits of the SageELF System:                                       

  • Reduces the complexity of RNA (cDNA) or protein samples
  • Allows construction of DNA libraries with multiple insert sizes for improved assembly
  • Allows sequencing of valuable DNA and retrieving sample for later study

Key Downstream Technologies: Proteomics, Mass Spec, Mate-pair sequencing, Splice variant detection

 

Check out Sage Science's tutorial videos for tips on using the Sage Science instruments.

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DNA Gel Casettes

Pre-Cast Agarose Gel Cassettes for DNA Size Selection

Gel cassettes kit are available in several configurations to provide a full range of options. Size select DNA in ranges between 100bp up to 1.5kb.  Also, High-Pass protocols are available for collecting all larger DNA above a fragment size.

  • 3% agarose, 100-250bp
  • 2% agarose, 100-600bp
  • 1.5% agarose, 300bp-1.5kb
  • 0.75% agarose, high-pass size selection

View the BluePippin cassette reference chart  to explore the DNA size selection options and run times.

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Protein Gel Cassettes

Pre-Cast SDS-Agarose Gel Cassettes for Protein Fractionation

Gel cassettes for protein fractionation are available in two agarose concentrations to provide a full range of options for proteomic studies.

Gel cassette types and target ranges:

  • 5% agarose, 10 – 100 kDa
    3% agarose, 50 – 500 kDa

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Pippin Pulse Electrophoresis Power Supply

Pulsed-field Power for Gel Boxes

Sage Science is pleased to introduce the new Pippin Pulse electrophoresis power supply for analytical gels. They’ve taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond. The Pippin Pulse features a simple software interface to provide users full flexibility to develop their own field-inversion protocols. The operating range is 25-175V , and up to 200 mA . The Pippin Pulse includes the power supply, cables, software (for PC) for programming, and user manual.

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TELL-Seq Bar Code Linked-Read Library Kit

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HLS2 HMW Library System

Extract.  React. Select.

Extract up to 1MB DNA directly from cells

The HLS2 system provides a new approach to DNA extraction. Users load cells suspensions or cell nuclei on Sage Science gel cassettes to perform lysis under electrophoretic conditions. This removes degraded and solubilized proteins but leaves intact DNA behind, bound in agarose. A cleavase is then used to carefully fragment the DNA into electrophoretically-mobile sizes. Automated DNA size selection collects the DNA in 6 size bins. Learn more about the technology.

Targeted Purification with CRISPR/Cas9:  CATCH*
CRISPR/Cas9 can be used to design targeted purification of large genomic regions or genes.  This in vitro application with Cas9, termed CATCH, can enhance study of structural variation and provide valuable phasing information.

*Cas9-Assisted Targeting of CHromosome Segments
Jiang et. al., 2015, Nature Communications, doi:10.1038/ncomm9101

Key Downstream Technologies: optical mapping, long read sequencing, bar-coded linked reads

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