PippinHT

See how ANU researchers are using thePippinHT for their DNA size selections

High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing | Sage Science

Authors: Ashley Jones, Cynthia Torkel, David Stanley, Jamila Nasim, Justin Borevitz, Benjamin Schwessinger

The authors provide a protocol for preparing high molecular weight DNA sequencing libraries with a particular focus on difficult plant and fungal tissues, and also can be used with animals and microbes. The libraries can be used for PacBio or Oxford Nanopore sequencing, includes clean up and size selection recommendations, in a manner that is economical and scalable.

Author Affiliations: Australian National University, Canberra, Australian Capital Territory, Australia

PLOS ONE
DOI: 10.1371/journal.pone.0253830

About the PippinHT

High Throughput Size Selection for Next-Gen Sequencing

Size Select Up To 24 Samples In One Run

PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing large-scale sequencing data.

Benefits of the PippinHT System		Key Downstream Technologies
Sample-to-sample reproducibility streamlines research Higher sample quality improves downstream analysis 24 sample capacity meets high throughput sequencing demands Cassettes can be partially used and then used for the next run		whole genome sequencing miRNA library isolation